| Project/Area Number |
21591255
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Hematology
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| Research Institution | Research Institute, International Medical Center of Japan |
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Principal Investigator |
YUO Akira 独立行政法人国立国際医療研究センター, 研究所・疾患制御研究部, 部長 (90221663)
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| Co-Investigator(Renkei-kenkyūsha) |
SAEKI Kumiko 独立行政法人国立国際医療研究センター, 研究所・疾患制御研究部, 室長 (80322717)
NISHIO Miwako 独立行政法人国立国際医療研究センター, 研究所・疾患制御研究部, 研究員 (30623318)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 再生医学 / 発生・分化 / ヒト万能細胞 / 造血幹細胞 / 分化誘導 |
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| Research Abstract |
We tried to induce hematopoietic cells from human pluripotent stem cells(ES cells and iPS cells) using non-feeder culture system. Our differentiation methods consist of two steps with initial sphere formation followed by secondary adherent culture. We succeeded in efficient production of neutrophils by low cell density culture, serum-free culture for hematopoietic induction, multilineage hematopoiesis with concomitant erythroid induction, production of virus-free safe human iPS cells, and hematopoietic induction from human iPS cells derived from hematopoietic progenitor cells.
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