| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2011: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2010: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2009: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Research Abstract |
To consider whether 3'UTR CS1 region prescribing stability of T-cell receptor zeta chain (TCR zeta) mRNA is associated with TCR zeta chain expression control, We made arm which is lack of CS1 and built a targeting vector. We introduced this targeting vector into an embryonic stem cell successively and made a recombinant embryonic stem cell. Furthermore, we injected it by the injection method and performed the manufacturing of chimera mouse. We crossbred this chimera mouse and wild-type mouse, and an F1 mouse was obtained. However, we tried the mating with the Cre mouse, but did not work well to remove pgk-neo cassette sandwiched in loxP incorporated in the genome of the F1 mouse.
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