Mechanism of systemic lupus erythematosus accompanied with the aberrant TCR zeta mRNA 3'UTR
| Project/Area Number |
21591268
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
膠原病・アレルギー・感染症内科学
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| Research Institution | Tokyo Dental College |
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Principal Investigator |
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| Project Period (FY) |
2009-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2011: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2010: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2009: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | TCR zeta / SLE / knockout mouse / 3'UTR / 全身性エリテマトーデス / TCR / シンデカン / EXTL2 / ノックアウトマウス / TCRζ鎖 / 3' UTR / RNA結合タンパク |
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| Research Abstract |
To consider whether 3'UTR CS1 region prescribing stability of T-cell receptor zeta chain (TCR zeta) mRNA is associated with TCR zeta chain expression control, We made arm which is lack of CS1 and built a targeting vector. We introduced this targeting vector into an embryonic stem cell successively and made a recombinant embryonic stem cell. Furthermore, we injected it by the injection method and performed the manufacturing of chimera mouse. We crossbred this chimera mouse and wild-type mouse, and an F1 mouse was obtained. However, we tried the mating with the Cre mouse, but did not work well to remove pgk-neo cassette sandwiched in loxP incorporated in the genome of the F1 mouse.
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Report
(6 results)
Research Products
(30 results)
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[Journal Article] Regulatory mechanisms for the production of BAFF and IL-6 are impaired in monocytes of patients of primary Sjögren's syndrome2011
Author(s)
Yoshimoto K, Tanaka M, Kojima M, Setoyama Y, Kameda H, Suzuki K, Tsuzaka K, Ogawa Y, Tsubota K, Abe T, Takeuchi T
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Journal Title
Arthritis Res Ther
Volume: 13(5)(Epub)
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