Molecular characteristic of Community-Acquired methicillin-resistant Staphylococcus aureus isolates by using New Genetic Diagnosis

• Project/Area Number: 21591294
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Infectious disease medicine
• Research Institution: Nagasaki University
• Principal Investigator: YANAGIHARA Katsunori 長崎大学, 大学院・医歯薬学総合研究科, 准教授 (40315239)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2011: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: MRSA / 分子疫学解析 / 薬剤耐性菌 / 病原因子 / 遺伝子診断

Research Abstract

This study is a nationwide survey of all clinical methicillin-resistant Staphylococcus aureus(MRSA) isolates, including community-acquired MRSA(CA-MRSA), in Japan. Only 4(2. 3%) of 171 SCCmec type IV strains were Panton-Valentine leukocidin(lukS/ F-PV)-positive. Thus, this result indicates a unique feature of SCCmec type IV strains in Japan. The information in this study not only is important in terms of local public health but will also contribute to an understanding of epidemic clones of CA-MRSA

Research Products

• [Journal Article] Antimicrobial susceptibility and molecular characteristic of 857 methicillin-resistant Staphylococcus aureus isolates from 16 medical centers in Japan(2008-2009): Nationwide survey of community-acquired and nosocomial MRSA (2012)
  • Author(s): Yanagihara K, Araki N, Watanabe S, Kinebuchi T, Kaku M, Maesakii S, Yamaguchi K, Matsumoto, Mikamo H, Takesue Y, Fujita J, Kadota J, Iwatsuki K, Hino H, Kaneko T, Asagoe K, Ikeda M, T Yasuoka A, Kohno S
  • Journal Title: Diagn Microbiol Infect Dis Volume: 72 Pages: 253-257
• [Journal Article] Antimicrobial susceptibility and molecular characteristic of 857 methicillin-resistant Staphylococcus aureus isolates from 16 medical centers in Japan (2008-2009) : Nationwide survey of community-acquired and nosocomial MRSA (2012)
  • Author(s): Yanagihara K, et.al
  • Journal Title: Diagn Microbiol Infect Dis Volume: 72 Pages: 253-257
  • Peer Reviewed
• [Journal Article] Clinical characteristics of bacteremia caused by methicillin-resistant Staphylococcus aureus in a tertiary hospital (2011)
  • Author(s): Yamada K, Yanagihara K, Hara Y, Araki N, Harada Y, Morinaga Y, Matsuda J, Izumikawa K, Seki M, Kakeya H, Yamamoto Y, Kohno S, Kamihira S
  • Journal Title: Tohoku J Exp Med Volume: 224 Pages: 61-67
• [Journal Article] High dose tobramycin inhibits lipopolysaccharide-induced MUC5AC production in human lung epithelial cells (2011)
  • Author(s): Nakamura S, Yanagihara K, Araki N, Morinaga Y, Yamada, K, Izumikawa K, Seki M, Kakeya H, Yamamoto Y, Kamihira S, Kohno
  • Journal Title: European Journal of Pharmacology Volume: 659 Pages: 67-71
  • NAID: 120006985129
• [Journal Article] Clinical characteristics of bacteremia caused by methicillin-resistant Staphylococcus aureus in a tertiary hospital (2011)
  • Author(s): Yamada K, Yanagihara K, et.al
  • Journal Title: Tohoku J Exp Med Volume: 242 Pages: 61-67
  • Peer Reviewed
• [Journal Article] High dose tobramycin inhibits lipopolysaccharide-induced MUC5AC production in human lung epithelial cells. European Journal of Pharmacology (2011)
  • Author(s): Nakamura S, Yanagihara K, et.al
  • Journal Title: European Journal of Pharmacology Volume: 659 Pages: 67-71
  • Peer Reviewed
• [Journal Article] Genetic diagnosis of community-acquired MRSA : A multiplex real-time PCR method for staphylococcal cassette chromosome mec typing and detecting toxin genes (2010)
  • Author(s): Motoshima M, Yanagihara K, Morinaga Y, Matsuda J, Sugahara K, Yamada Y, Kohno S, Kamihira S
  • Journal Title: Tohoku J Exp Med Volume: 220 Pages: 165-170
  • NAID: 10027083430
• [Journal Article] Evaluation of pathogen detection from clinical samples by real-time polymerase chain reaction using a sepsis pathogen DNA detection Kit (2010)
  • Author(s): Yanagihara K, Kitagawa Y, Tomonaga M, Tsukasaki K, Kohno S, Seki M, Sugimoto H, Shimazu T, Tasaki O, Matsushima A, Ikeda Y, Okamoto S, Aikawa N, Hori S, Obara H, Ishizaka A, Hasegawa N, Takeda J, Kamihira S, Sugahara K, Asari S, Murata M, Kobayashi Y, Gin
  • Journal Title: Critical Care Volume: 14
• [Journal Article] Evaluation of Pathogen Detection from Clinical Samples by Real-Time PCR using a Sepsis Pathogen, DNA Detection Kit. (2010)
  • Author(s): Yanagihara K, et al
  • Journal Title: Critical Care. Volume: 14(4)
  • Peer Reviewed
• [Journal Article] Azithromycin inhibits nontypeable Haemophilus infuenzae-induced MUC5AC expression and secretion via inhibition of activator protein -1 in human airway epithelial cells (2010)
  • Author(s): Araki N, Yanagihara, et al
  • Journal Title: European Journal of Pharmacology Volume: 644 Pages: 209-214
  • Peer Reviewed
• [Journal Article] Genetic Diagnosis of Community-Acquired MRSA : A Multiplex Real-Time PCR Method for Staphylococcal Cassette Chromosome mec Typing and Detecting Toxin Genes (2010)
  • Author(s): Motoshima M, et al.
  • Journal Title: Tohoku J Exp Med 220 Pages: 165-70
  • Peer Reviewed
• [Journal Article] Efficacy of linezolid against PVL-positive MRSA in a mouse model of hematogenous pulmonary infection (2009)
  • Author(s): Yanagihara K, Kihara R, Araki N, Morinaga Y, Seki M, Izumikawa K, Kakeya H, Yamamoto Y, Yamada Y, Kamihira S, Tsukamoto K, Kohno S
  • Journal Title: Int J Antimicrob Agents Volume: 34 Pages: 477-481
• [Journal Article] Efficacy of linezolid against PVL-positive MRSA in a mouse model of hematogenous pulmonary infection (2009)
  • Author(s): Yanagihara K, et.al
  • Journal Title: Int J Antimicrob Agents 34 Pages: 477-81
  • Peer Reviewed