Analysis on mouse model of Mycoplasma pneumoniae pneumonia, and its application to treatment.

• Project/Area Number: 21591298
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Infectious disease medicine
• Research Institution: Kyorin University
• Principal Investigator: GOTO Hajime 杏林大学, 医学部, 教授 (80134617)
• Co-Investigator(Kenkyū-buntansha): WADA Hiroo 杏林大学, 医学部, 講師 (50407053)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 感染症治療学 / 感染症 / シグナル伝達 / フイニプラズマ肺炎 / デキサメサゾン / クラリスロマイシン / マイコプラズマ肺炎 / マイコプラズマ

Research Abstract

We generate a mouse model for Mycoplasma pneumoniae pneumonia, using M. pneumoniae extract(Mp extract). Cell culture experiments demonstrated that Mp extract activated intracellular signals, associated with p38, PI-3K and NF-kB. Mouse model histologically yielded the severe human M. pneumoniae pneumonia, as both share lymph-plasma cytic peri-bronchovascular infiltration. Our results on the effect of either dexamethasone, clarithromycin or both further demonstrated that Mycoplasma pneumoniae infection caused both NF-kB-dependent and NF-kB independent reactions.

Research Products

• [Journal Article] マイコプラズマ感染症の診断:新しいLAMP法による診断について (2012)
  • Author(s): 和田裕雄、大西宏明、後藤元
  • Journal Title: Laboratory and Clinical Practice Volume: 29 Pages: 91-98
• [Journal Article] 和田裕雄、大西宏明、後藤元「マイコプラズマ感染症の診断:新しいLAMP法による診断について」 (2012)
  • Author(s): 和田裕雄、後藤元, ら
  • Journal Title: Laboratory and Clinical Practice Volume: 29 Pages: 91-98
• [Journal Article] Inflammation provoked by Mycoplasma pneumoniae extract : implications for combination treatment with clarithromycin and dexamethasone (2011)
  • Author(s): Hirao S, Wada H, Nakagaki K, Saraya T, Kurai D, Mikura S, Yasutake T, Higaki M, Yokoyama T, Ishii H, Nakata K, Akashi T, Kamiya S and Goto H
  • Journal Title: FEMS Immunology & Medical Microbiology Volume: 62 Pages: 182-189
• [Journal Article] Identification of a mechanism for lung inflammation caused by Mycoplasma pneumoniae using a novel mouse model (2011)
  • Author(s): Saraya T, Nakata K, Nakagaki K, Motoi N, Iihara K, Fujioka Y, Oka T, Kurai D, Wada H, Ishii H, Taguchi H, Kamiya S and Goto H
  • Journal Title: Results in Immunology Volume: 1 Pages: 76-87
• [Journal Article] Inflammation provoked by Mycoplasma pneumoniae extract : implications for combination treatment with clarithromycin and dexamethasone" 2011 ; 62 : 182-189 (2011)
  • Author(s): Hirao S, Wada H, Goto H, et al
  • Journal Title: FEMS Immunology & Medical Microbiology Volume: 62 Issue: 2 Pages: 182-189
  • DOI: 10.1111/j.1574-695x.2011.00799.x
  • Peer Reviewed
• [Journal Article] Identification of a mechanism for r lung inflammation caused by Mycoplasma pneumoniae using a novel mouse model (2011)
  • Author(s): Saraya T, Wada H, Goto H
  • Journal Title: Results in Immunology Volume: 1 Issue: 1 Pages: 76-87
  • DOI: 10.1016/j.rinim.2011.11.001
  • Peer Reviewed
• [Journal Article] Multicenter surveilcance of adult atypical pneumonia in Japan : its clinical features, and efficacy and safety of clarithromycin (2011)
  • Author(s): Goto H
  • Journal Title: Journal of Infection and Chemotherapy Volume: 17 Pages: 97-104
  • Peer Reviewed
• [Journal Article] Nationwide surveillance of bacterial respiratory pathogens conducted by the Japanese society of chemotherapy in 2007 : general view of the pathogens' antibacterial susceptibility (2009)
  • Author(s): Niki Y, Goto H, et al.
  • Journal Title: Journal of Infection and Chemotherapy 15 Pages: 156-167
  • Peer Reviewed
• [Journal Article] The Japanese Respiratory Society guidelines for the management of hospital acquired pneumonia in adults 2008. (2009)
  • Author(s): Kohno S, Goto H, et al.
  • Journal Title: Respirology 14
  • Peer Reviewed
• [Journal Article] Antimicrobial susceptibility of pathogens isolated from more than 10,000 patients with infectious respiratory diseases : a25-year longitudinal stday. (2009)
  • Author(s): Goto H, et al.
  • Journal Title: Journal of Infection and Chemotherapy 15 Pages: 347-360
  • Peer Reviewed
• [Journal Article] Immunological analysis and pathological examination of gnotobiotic mice monoassociated with Mycoplasma pneumoniae (2009)
  • Author(s): Sekine H, Goto H, et al.
  • Journal Title: Journal of Medical Biology 58 Pages: 697-705
  • Peer Reviewed
• [Journal Article] Mycobacterium kyorinense sp.Nov related to Mycobacterium celatum, isolated from human clinical specimens (2009)
  • Author(s): Okazaki M, Wada H, Goto H, et al.
  • Journal Title: International Journal of Systematic and Evolutionary Microbiology 59 Pages: 1336-1341
  • Peer Reviewed
• [Journal Article] Isolation of Mycobacterium kyorinense in a patient with respiratory failure (2009)
  • Author(s): Wada H, Goto H, et al.
  • Journal Title: Ann Intern Med 150 Pages: 568-570
  • Peer Reviewed
• [Journal Article] A protein deacetylase SIRT1 is a negative regulator of metalloproteinase-9 (2009)
  • Author(s): Nakamaru Y, Wada H, Goto H, et al.
  • Journal Title: The FASEB Journal 23 Pages: 2810-2819
  • Peer Reviewed
• [Presentation] クラリスロマイシンとデキサメサゾンの共投与がマイコプラズマ肺炎モデルへ及ぼす影響の検討 (2010)
  • Author(s): 平尾晋、和田裕雄、檜垣学、安武哲生、三倉真一郎、皿谷健、倉井大輔、中垣和英、明石敏、神谷茂、後藤元
  • Organizer: 第48回日本呼吸器学会総会
  • Place of Presentation: 京都
  • Year and Date: 2010-04-25
• [Presentation] マイコプラズマ抽出液の刺激によるRAW264.7細胞のIL-6およびTNF-α産生炎症惹起作用の検討 (2010)
  • Author(s): 和田裕雄、平尾晋、三倉真一郎、安武哲生、檜垣学、倉井大輔、皿谷健、石井晴之、明石敏、中垣和英、神谷茂、後藤元
  • Organizer: 第48回日本呼吸器学会総会
  • Place of Presentation: 京都
  • Year and Date: 2010-04-25
• [Presentation] マイコプラズマ肺炎マウスモデルにおけるIL-23の関与について (2010)
  • Author(s): 倉井大輔, 和田裕雄, 皿谷健, 中垣和英, 神谷茂, 藤岡保範, 後藤元
  • Organizer: 第48回日本呼吸器学会総会
  • Place of Presentation: 京都
  • Year and Date: 2010-04-25
• [Presentation] Mycoplasma pneumoniae extract induced IL-6 and TNF-a production by RAW264.7 cells (2009)
  • Author(s): Wada H, Goto H, et al.
  • Organizer: European Respiratory Society Congress
  • Place of Presentation: ウィーン
  • Year and Date: 2009-09-21
• [Presentation] Co-treatment with clarithromycin and dexamethasone on murine Mycoplasma pneumoniae pneumonia model (2009)
  • Author(s): Hirao S, Wada H, Goto H, et al.
  • Organizer: European Respiratory Society Congress
  • Place of Presentation: ウィーン
  • Year and Date: 2009-09-21
• [Presentation] Co-treatment with clarithromycin and dexamethasone on murine Mycoplasma pneumoniae pneumonia model (2009)
  • Author(s): Hirao S, Wada H, Mikura S, Saraya T, Kurai D, Akashi T, Nakagaki K, Kamiy S, Goto H
  • Organizer: ERS 2009
  • Place of Presentation: Vienna
  • Year and Date: 2009-09-14
• [Presentation] Mycoplasma pneumoniae extract induced IL-6 and TNF-alpha production by RAW264.7 cells (2009)
  • Author(s): Wada H, Hirao S, Mikura S, Saraya T, Kurai D, Akashi T, Nakgaki K, Kamiya S, Goto H
  • Organizer: ERS 2009
  • Place of Presentation: Vienna
  • Year and Date: 2009-09-14
• [Presentation] 呼吸器感染症分離菌の感受性推移 (2009)
  • Author(s): 後藤元
  • Organizer: 第83回日本感染症学会総会・会長講演
  • Place of Presentation: 東京
  • Year and Date: 2009-04-24
• [Presentation] マイコプラズマ菌体抽出物による炎症反応に対するクラリスロマイシンの効果について (2009)
  • Author(s): 平尾晋、和田裕雄、皿谷健、三倉真一郎、倉井大輔、石井晴之、中垣和英、明石敏、後藤元
  • Organizer: 第83回日本感染症学会総会
  • Place of Presentation: 東京
  • Year and Date: 2009-04-23
• [Remarks]
  • URL: http://www.kyorin-u.ac.jp/univ/graduate/medicine/education/departments/respirmed/
• [Remarks]
  • URL: http://www.kyorin-u.ac.jp/univ/graduate/medicine/education/departments/respir-med/
• [Remarks]
  • URL: http://www.kyorin-u.ac.jp/univ/graduate/medicine/education/departments/respir-med/
• [Remarks]
  • URL: http://www.kyorin-u.ac.jp/univ/graduate/medicine/departments/respir_med.php