Activation of intestinal mucosal immunization via probiotic strain with the mechanism of Cd1d and NKT cell

• Project/Area Number: 21591301
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Infectious disease medicine
• Research Institution: Tokyo Medical University
• Principal Investigator: MATSUMOTO Tetsuya 東京医科大学, 医学部, 教授 (10256688)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 腸管免疫 / 腸内細菌 / bacterial translocation / 免疫不全 / CD1d / NKT細胞 / 菌血症 / プロバイオティクス / germ-free / 緑膿菌

Research Abstract

We studied the host mechanisms involved in controlling intestinal colonization with commensal bacteria. Under specific pathogen. free or germ-free conditions, intragastric administration of Pseudomonas aeruginosa, E. coli, Staphylococcus aureus, or Lactobacillus gasseri resulted in increased colonization of the small intestine and bacterial translocation in mice lacking Cd1d, compared with wild type mice. In contrast, activation of Cd1d-restricted T cells(NKT cells) withα-galactosylceramide caused diminished intestinal colonization with the same bacterial strains. In vitro data suggest that NKT cells were shown to induce the release of lysozyme from intestinal crypts. These data support a role for Cd1d in regulating intestinal colonization through mechanisms that include the control of Paneth cell function.

Research Products

• [Journal Article] Inhibitory effects of lactoferrin on biofilm formation in clinical isolates of Pseudomonas aeruginosa (2012)
  • Author(s): Kamiya H, Ehara T, Matsumoto T
  • Journal Title: J Infect Chemother Volume: 18 Issue: 1 Pages: 47-52
  • DOI: 10.1007/s10156-011-0287-1
  • NAID: 10030226366
  • Peer Reviewed
• [Journal Article] Measuring antimicrobial susceptibility of Pseudomonas aeruginosa using Poloxamer 407 gel (2011)
  • Author(s): Yamada H, Koike N, Ehara T, Matsumoto T
  • Journal Title: J Infect Chemother Volume: 17 Issue: 2 Pages: 195-199
  • DOI: 10.1007/s10156-010-0109-x
  • NAID: 10028171380
  • Peer Reviewed
• [Journal Article] Oral administration of heat-killed Lactobacillus plantarum strain b240 protects mice against Salmonella enterica serovar Typhimurium.Bioscience (2010)
  • Author(s): Ishikawa H
  • Journal Title: Biotechnology, and Biochemistry Volume: 74 Pages: 1338-1342
  • Peer Reviewed
• [Journal Article] Involvement of endotoxin in the mortality of mice with gut-derived sepsis due to Methicillin Resistant Staphylococcus aureus (2010)
  • Author(s): Uramatsu M
  • Journal Title: Microbiology & Immunology Volume: 54 Pages: 330-337
  • Peer Reviewed
• [Journal Article] Cd1d-dependent regulation of bacterial colonization in the intestine of mice (2009)
  • Author(s): Nieuwenhuis EE, Matsumoto T, Lindenbergh D, Willemsen R, Kaser A, Simons-Oosterhuis Y, Brugman S, Yamaguchi K, Ishikawa H, Aiba Y, Koga Y, Samsom JN, Oshima K, Kikuchi M, Escher JC, Hattori M, Onderdonk AB, Blumberg RS
  • Journal Title: J Clin Invest Volume: 119(5) Issue: 5 Pages: 1241-50
  • DOI: 10.1172/jci36509
• [Journal Article] CD1d-dependent regulation of bacterial colonization in the intestine. Journal of Clinical Investigation (2009)
  • Author(s): Nieuwenhuis EES, Matsumoto T
  • Journal Title: J Clin Invest 119 Pages: 1241-1250
  • Peer Reviewed
• [Journal Article] Interleukin-1 deficiency in combination with macrophage depletion increases susceptibility to Pseudomonas aeruginosa bacteremia (2009)
  • Author(s): Horino T
  • Journal Title: Microbiol Immunol 53 Pages: 502-511
  • Peer Reviewed
• [Journal Article] Characteristics of a novel Pseudomonas aeruginosa bacterio phage, PAJU2, which is genetically related to bacteriophage D3 (2009)
  • Author(s): Uchiyama J
  • Journal Title: Virus Res 139 Pages: 131-134
  • Peer Reviewed
• [Presentation] 多剤耐性菌をめぐる最近の話題 (2012)
  • Author(s): 松本哲哉
  • Organizer: 第86回日本感染症学会総会学術講演会(イブニングセミナー)
  • Year and Date: 2012-04-25
• [Presentation] MRSAを中心とするグラム陽性球菌感染症 (2012)
  • Author(s): 松本哲哉
  • Organizer: 第109回日本内科学会講演会(シンポジウム)
  • Place of Presentation: 京都
  • Year and Date: 2012-04-15
• [Presentation] ムチン分解能とトランスロケーション能の有無に関するビフィズス菌の安全性評価 (2011)
  • Author(s): 武藤正達
  • Organizer: 第15回腸内細菌学会
  • Place of Presentation: 東京
  • Year and Date: 2011-06-16
• [Presentation] Antimicrobial resistance surveillance in Japan (2010)
  • Author(s): Matsumoto T
  • Organizer: 12th Western Pacific Congress on Chemotherapy and Infectious Diseases
  • Place of Presentation: シンガポール
  • Year and Date: 2010-12-05
• [Presentation] ホスト因子を考慮した治療法(シンポジウム) (2010)
  • Author(s): 松本哲哉
  • Organizer: 第58回日本化学療法学会総会
  • Place of Presentation: 長崎
  • Year and Date: 2010-06-03
• [Presentation] 難治性感染症とその要因-免疫不全、バイオフィルムとの関連- (2010)
  • Author(s): 松本哲哉
  • Organizer: 第54回日本リウマチ学会総会・学術集会
  • Place of Presentation: 神戸
  • Year and Date: 2010-04-23
• [Presentation] Drug-resistant respiratory pathogens in Japan (2009)
  • Author(s): Matsumoto T
  • Organizer: The 6th Annual Meeting Showa International Symposium for Life Sciences
  • Place of Presentation: 昭和大学(東京)
  • Year and Date: 2009-09-12
• [Book] Current Topics of Infectious Diseases in Japan and Asia (2010)
  • Author(s): Matsumoto T
  • Total Pages: 132
  • Publisher: Springer
• [Book] 院内感染対策(医療関連感染対策) (2009)
  • Author(s): 松本哲哉
  • Total Pages: 531
  • Publisher: 東京都福祉保健局
• [Remarks]
  • URL: http://www.tokyo-med.ac.jp/microbiology/
• [Remarks]
  • URL: http://www.tokyo-med.ac.jp/microbiology/
• [Remarks]
  • URL: http://www.tokyo-med.ac.jp/microbiology/
• [Remarks]
  • URL: http://www.tokyo-med.ac.jp/microbiology/publication2.html