Medical verification of Hansen disease in the past 70 years : genetic analysis of Leprosy, Tuberculosis and Hepatitis C virus
| Project/Area Number |
21591303
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Infectious disease medicine
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| Research Institution | Fujita Health University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
TSUTSUMI Yutaka 藤田保健衛生大学, 医学部, 教授 (80138643)
MAKINO Masanao 藤田保健衛生大学, 医学部, 客員教授 (00116097)
KAMAHORA Toshio 鳥取大学, 医学部, 准教授 (30144520)
SHIOGAMA Kazuya 藤田保健衛生大学, 医学部, 助教 (10387699)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | ハンセン病 / C型肝炎 / 結核 / 感染症学 |
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| Research Abstract |
The review of the the pathological data of a total of 996 patients autopsied in Oku-Komyo-En(1940-1999) found that the dramatic increase in the incidence of cirrhosis of the liver and HCC(1960-1999). In patients with cirrhosis of the liver, total RNA was examined by nested RT-PCR. The HCV genotypes were 85.7% for 1b(30/35), and 14.3% for 2a(5/35) in patients with leprosy. Tissues of 30 patients with leprosy(HCV genotype 1b) were amplified in 12 of the samples(NS5B region). Recombinant clones were then sequenced and the phylogenetic analysis of NS5 sequences was carried out. Our data suggest that at least three strains of HCV existed in this sanatorium. This observation strongly suggests the horizontal transmission of HCV in the past 70 years in this leprosy sanatorium.
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Report
(4 results)
Research Products
(8 results)
