Elucidation of mechanisms of aggravation in Influenza virus encephalopathy using with tissue culture system
Project/Area Number |
21591330
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Pediatrics
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Research Institution | Fukushima Medical University |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
KAWASAKI Yukihiko 福島県立医科大学, 医学部, 准教授 (00305369)
HASHIMOTO Koichi 福島県立医科大学, 医学部, 准教授 (50322342)
|
Project Period (FY) |
2009 – 2011
|
Project Status |
Completed (Fiscal Year 2011)
|
Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
Keywords | 小児感染症学 / インフルエンザ脳症 / アポトーシス / 血管内皮細胞 / サイトカイン / 細胞透過性 / タイトジャンクション / ヒト臍帯静脈血管内皮細胞 / TNF-α / 経上皮内皮電気抵抗値 / ウェスタンプロット / RNA / 消炎鎮痛剤 / MTT法 / 細胞毒性 / インフルエンザ / 脳症 |
Research Abstract |
We examined induction of apoptosis, cytotoxicity, and change of tight junction due to TNF-α and non-steroidal anti-inflammatory drugs in model of Influenza virus encephalopathy using with human umbilical vein vascular endothelial cell(HUVEC). We evaluated influence of TNF-α to tight junction of HUVEC by western blot for Claudin-5 and Occludin, and by measurement of trans-endothelial epithelium endodermis electric resistance(TER) for alteration of cell permeability.
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Report
(4 results)
Research Products
(22 results)
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[Journal Article] Rhinovirus load and disease severity in children with lower respiratory tract infections2012
Author(s)
Takeyama A, Hashimoto K, Sato M, Sato T, Kanno S, Takano K, Ito M, Katayose M, Nishimura H, Kawasaki Y, Hosoya M
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Journal Title
J Med Virol
Volume: 84巻
Pages: 1135-42
Related Report
Peer Reviewed
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[Journal Article] Survey of subacute sclerosing panencephalitis in Japan.2012
Author(s)
Abe Y, Hashimoto K, Iinuma K, Ohtsuka Y, Ichiyama T, Kusuhara K, Nomura K, Mizuguchi M, Aiba H, Suzuki Y, Mizusawa H, Hosoya M.
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Journal Title
Related Report
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