The function of LARG in the nucleus

• Project/Area Number: 21591348
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Pediatrics
• Research Institution: The University of Tokyo
• Principal Investigator: SUZUKI Nobuchika 東京大学, 先端科学技術研究センター, 特任助教 (90247007)
• Co-Investigator(Kenkyū-buntansha): KOZASA Tohru 東京大学, 先端科学技術センター, 特任教授 (70202059) ; KAWAMURA Takeshi 東京大学, 先端科学技術センター, 特任助教 (70306835)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 小児血液学 / 白血病細胞 / 分化 / RH-RhoGEF / G蛋白質 / ショットガンプロテオミクス / リン酸化

Research Abstract

The signal through p115RhoGEF in the nucleus, collaborating with the canonical Gα13~p115RhoGEF～RhoA pathway at the plasma membrane, may regulate the differentiation of HL60 cells.

Research Products

• [Journal Article] Signalling mechanisms of RhoGTPase regulation by the heterotrimeric G proteins G12 and G13. (2011)
  • Author(s): KOZASA, T., HAJICEK, N., CHOW, C. R. & SUZUKI, N.
  • Journal Title: J Biochem Volume: 150 Pages: 357-69
  • NAID: 10030587857
• [Journal Article] A method of generating antibodies against exogenously administered self-antigen by manipulating CD4+CD25+ regulatory T cells. (2011)
  • Author(s): IWANARI, H., NAKADA-NAKURA, Y., KUSANO-ARAI, O., SUZUKI, N., KODAMA, T., SAKIHAMA, T. & HAMAKUBO, T.
  • Journal Title: J Immunol Methods Volume: 369 Pages: 108-14
• [Journal Article] Signalling mechanisms of RhoGTPase regulation by the heterotrimeric G proteins G12 and G13 (2011)
  • Author(s): Kozasa T, Hajicek N, Chow CR, Suzuki N
  • Journal Title: J Biochem Volume: 150 Issue: 4 Pages: 357-369
  • DOI: 10.1093/jb/mvr105
  • NAID: 10030587857
  • Peer Reviewed
• [Journal Article] A method of generating antibodies against exogenously administered self-antigen by manipulating CD4+CD25+ regulatory T cells (2011)
  • Author(s): Iwanari H
  • Journal Title: J Immunol Method Volume: Jun30 ; 369(1-2) Issue: 1-2 Pages: 108-14
  • DOI: 10.1016/j.jim.2011.04.011
  • Peer Reviewed
• [Journal Article] Regulation and physiological functions of G12/13-mediated signaling pathways. (2009)
  • Author(s): SUZUKI, N., HAJICEK, N. & KOZASA, T.
  • Journal Title: Neurosignals Volume: 17 Pages: 55-70
• [Journal Article] Activation of leukemia-associated RhoGEF by Galpha13 with significant conformational rearrangements in the interface. (2009)
  • Author(s): SUZUKI, N., TSUMOTO, K., HAJICEK, N., DAIGO, K., TOKITA, R., MINAMI, S., KODAMA, T., HAMAKUBO, T. & KOZASA, T.
  • Journal Title: J Biol Chem Volume: 284 Pages: 5000-9
• [Presentation] The role of p115RhoGEF during differentiation of human myeloid HL60 leukemia cells (2012)
  • Organizer: Gordon Research conference, Phosphorylation & G-Protein Mediated Signaling Networks
  • Place of Presentation: University of New England
• [Presentation] The role of the RH-RhoGEF signaling pathway in differentiation of human myeloid HL60 leukemia cells (2011)
  • Organizer: 日本生化学会
  • Place of Presentation: 京都
  • Year and Date: 2011-09-24
• [Presentation] The role of the G13～RH-RhoGEF signal pathway in differentiation of human myeloid HL60 leukemia cells (2010)
  • Author(s): 鈴木信周
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-10
• [Presentation] The role of the G13～RH-RhoGEF signal pathway in differentiation of human myeloid HL60 leukemia cells (2010)
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸ポートアイランド
• [Presentation] Biochemical analysis of Gaq-Y261L mutant using a new Gai/q chimera, Gai/qL (2009)
  • Author(s): Takeharu Kawano
  • Organizer: Gordon Research Conference
  • Place of Presentation: New England Univ, Port land, US
  • Year and Date: 2009-06-08
• [Remarks]
  • URL: http://www.lsbm.org/
• [Remarks]
  • URL: http://www.lsbm.org/
• [Remarks]
  • URL: http://www.lsbm.org/
• [Remarks]
  • URL: http://www.rcast.u-tokyo.ac.jp/ja/