Modulation of mRNA splicing for the genetic diseases

• Project/Area Number: 21591365
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Pediatrics
• Research Institution: Kawasaki Medical School
• Principal Investigator: KURIBAYASHI Futoshi 川崎医科大学, 医学部, 教授 (60251443)
• Co-Investigator(Kenkyū-buntansha): YAMAUCHI Akira 川崎医科大学, 医学部, 准教授 (80372431)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 小児免疫 / アレルギー / 膠原病学 / 活性酸素 / 慢性肉芽腫症 / 好中球

Research Abstract

The gaol of this research is an analysis of transcription, post-transcription and the modulation of mRNA. This researches clarified transcriptional regulation of the phagocyte NADPH oxidase(J. Immunol. 2011, BBRC 2010) and signal transduction by oxidative stress for the cells(BBRC 2011). This reaearch further developed the method to detect superoxide quantitatively(Tropical Medicine and Health. 2011).

Research Products

• [Journal Article] Review : The phagocyte NADPH oxidase and bacterial infections (2012)
  • Author(s): Akira YAMAUCHI, Akitoyo ICHINOSE, Chikage KAWAI, and Futoshi KURIBAYASHI
  • Journal Title: Kawasaki Medical Journal
  • Peer Reviewed
• [Journal Article] The phagocyte NADPH oxidase and bacterial infections (Accepted for publication on the 17th of January in 2012) (2012)
  • Author(s): Akira Yamauchi
  • Journal Title: Kawasaki Medical Journal Volume: (In press)
  • Peer Reviewed
• [Journal Article] Possible involvement of Helios in controlling the immature B cell functions via transcriptional regulation of protein kinase Cs Results in Immunology (2011)
  • Author(s): Hidehiko Kikuchi, Masami Nakayama, Yasunari Takami, Futoshi Kuribayashi, Tatsuo Nakayama
  • Journal Title: Results in Immunology Volume: 1 Issue: 1 Pages: 88-94
  • DOI: 10.1016/j.rinim.2011.11.002
  • Peer Reviewed
• [Journal Article] A highly sensitive chemiluminescence assay for superoxide detection and chronic granulomatous disease diagnosis (2011)
  • Author(s): Tsuyoshi Yamazaki, Chikage Kawai, Akira Yamauchi, and Futoshi Kuribayashi
  • Journal Title: Tropical Medicine and Health Volume: 39 Issue: 2 Pages: 41-45
  • DOI: 10.2149/tmh.2011-08
  • NAID: 10029327277
  • ISSN: 1348-8945, 1349-4147
  • Peer Reviewed
• [Journal Article] GCN5 regulates the activation of PI3K/Akt survival pathway in B cells exposed to oxidative stress via controlling gene expressions of Syk and Btk (2011)
  • Author(s): Hidehiko Kikuchi, Futoshi Kuribayashi, Yasunari Takami, Shinobu Imajoh-Ohmi, and Tatsuo Nakayama
  • Journal Title: Biochem Biophys Res Commun Volume: 405 Issue: 4 Pages: 657-661
  • DOI: 10.1016/j.bbrc.2011.01.088
  • Peer Reviewed
• [Journal Article] GCN5 regulates the superoxide generating system in leukocytes via controlling gp91-phox gene expression (2011)
  • Author(s): Hidehiko Kikuchi, Futoshi Kuribayashi, Naomi Kiwaki, Yasunari Takami, and Tatsuo Nakayama
  • Journal Title: J. Immunol Volume: 186 Issue: 5 Pages: 3015-3022
  • DOI: 10.4049/jimmunol.1000364
  • Peer Reviewed
• [Journal Article] Adult-onset hereditary pulmonary alveolar proteinosis caused by a single-base deletion in CSF2RB (2011)
  • Author(s): Tanaka, T., et al
  • Journal Title: J Med Genet Volume: 48 Issue: 3 Pages: 205-209
  • DOI: 10.1136/jmg.2010.082586
  • Peer Reviewed
• [Journal Article] GCN5 regulates the superoxide generating system in leukocytes via controlling gp91-phox gene expression (2011)
  • Author(s): Hidehiko Kikuchi
  • Journal Title: J.Immunol. Volume: 186 Pages: 3015-3022
  • Peer Reviewed
• [Journal Article] Adult-onset hereditary pulmonary alveolar proteinosis caused by a single-base deletion in CSF2RB (2011)
  • Author(s): Takeshi Tanaka
  • Journal Title: J.Med.Genet. Volume: 48 Pages: 205-209
  • Peer Reviewed
• [Journal Article] Curcumin dramatically enhances retinoic acid-induced superoxide generating activity via accumulation of p47-phox and p67-phox proteins in U937 cells. (2010)
  • Author(s): Hidehiko Kikuchi, Futoshi Kuribayashi, Naomi Kiwaki and Tatsuo Nakayama
  • Journal Title: Biochem Biophys Res Commun Volume: 395 Issue: 1 Pages: 61-65
  • DOI: 10.1016/j.bbrc.2010.03.136
  • Peer Reviewed
• [Journal Article] Curcumin dramatically enhances retinoic acid-induced superoxide generating activity via accumulation of p47-phox and p67-phox proteins in U937 cells (2010)
  • Author(s): Hidehiko Kikuchi, Futoshi Kuribayashi, Naomi Kiwaki, Tatsuo Nakayama
  • Journal Title: Biochem.Biophys.Res.Commun. 395 Pages: 61-65
  • Peer Reviewed
• [Presentation] Robust directionality of neutrophil chemotaxis to fMLP is due to holding up the morphology (2012)
  • Author(s): Akira Yamauchi
  • Organizer: 日本細菌学会
  • Place of Presentation: 長崎
  • Year and Date: 2012-03-29
• [Presentation] Robust directionality of neutrophil chemotaxis to fMLP is due to holding up the morphology (2012)
  • Author(s): Akira Yamauchi
  • Organizer: 第85回日本細菌学会
  • Place of Presentation: 長崎市
  • Year and Date: 2012-03-29
• [Presentation] 好中球は走化性因子によって遊走パターンが異なる (2012)
  • Author(s): 山内明
  • Organizer: 分子予防環境医学研究会
  • Place of Presentation: 倉敷
  • Year and Date: 2012-01-27
• [Presentation] 好中球は走化性因子によって遊走パターンが異なる (2012)
  • Author(s): 山内明
  • Organizer: 第11回分子予防環境医学研究会
  • Place of Presentation: 倉敷市
  • Year and Date: 2012-01-27
• [Presentation] MOLECULAR CLONING AND CHARACTERIZATION OF A STEROID RECEPTOR-BINDING PROTEIN (2010)
  • Author(s): Mitsunori Ikeda
  • Organizer: SRB-RGS cDNA日本生化学会・日本分子生物学会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-10
• [Presentation] MOLECULAR CLONING AND CHARACTERIZATION OF A STEROID RECEPTOR-BINDING PROTEIN, SRB-RGS cDNA (2010)
  • Author(s): Mitsunori Ikeda
  • Organizer: 日本生化学会・日本分子生物学会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-07
• [Presentation] 好中球の遊走パターンと遊走中の形態解析 (2010)
  • Author(s): 山内明
  • Organizer: 細胞機能可視化研究会研究集会
  • Place of Presentation: 東京
  • Year and Date: 2010-09-21
• [Presentation] TAXIScan法によるヒトリンパ球の遊走解析 (2010)
  • Author(s): 山内明
  • Organizer: 日本免疫毒性学会学術大会
  • Place of Presentation: つくば市
  • Year and Date: 2010-09-10
• [Presentation] 活性酸素を生成する食細胞NADPHオキシダーゼの発現解析 (2010)
  • Author(s): 山内明
  • Organizer: 日本生体防御学会(シンポジウム)
  • Place of Presentation: 仙台
  • Year and Date: 2010-07-24
• [Presentation] 活性酸素を生成する食細胞NADPHオキシダーゼの発現解析 (2010)
  • Author(s): 栗林太
  • Organizer: 第21回日本生体防御学会(シンポジウム)
  • Place of Presentation: 仙台
  • Year and Date: 2010-07-23
• [Presentation] 新規細胞動態解析装置TAXIScan技術の開発と応用 (2010)
  • Author(s): 山内明
  • Organizer: 新規細胞動態解析装置TAXIScan技術の開発と応用
  • Place of Presentation: 岡山市
  • Year and Date: 2010-06-15
• [Presentation] Lucigenin-enhanced CL depends on adhered granulocytes (2009)
  • Author(s): 栗林太
  • Organizer: 日本分子生物学会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-09
• [Presentation] GCN5 regulates the superoxide producing system in leukocytes (2009)
  • Author(s): Naomi Kiwaki
  • Organizer: 日本分子生物学会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-09
• [Presentation] Lucigenin-enhanced chemiluminescence depends on adhered granulocytes (2009)
  • Author(s): 栗林太, 川井千景, 山内明, 山崎剛, 大海忍
  • Organizer: 日本分子生物学会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-09
• [Book] Functions of the phagocyte NADPH oxidase and Chronic Granulomatous Disease(CGD) Nova Science Publishers(In : Neutrophils : Lifespan, Functions and Roles in Disease) (2010)
  • Author(s): Akira Yamauchi and Futoshi Kuribayashi
• [Book] Neutrophils : Lifespan, Functions and Roles in Disease (2010)
  • Author(s): Akira Yamauchi, Futoshi Kuribayashi
  • Total Pages: 7
  • Publisher: Nova Science Publishers(査読有り Accepted for publication in December 19, 2009)