| Project/Area Number |
21591367
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pediatrics
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| Research Institution | Kagoshima University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
OKAMOTO Yasuhiro 鹿児島大学, 医学部歯学部附属病院, 講師 (30398002)
SHINKODA Yuichi 鹿児島大学, 医学部・歯学部附属病院, 特任助教 (50457652)
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| Co-Investigator(Renkei-kenkyūsha) |
HIRAO Atsushi 金沢大学, がん研究所, 教授 (90343350)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 造血幹細胞 / 造血幹細胞移植 / CD34陽性細胞 / 加齢 / 老化 / 転写因子 / ヒト造血幹細胞 / 造血細胞移植術 / 加齢現象 / IKAROS / CEPBPAα / リンパ球/骨髄球比 / 造血細胞移植 / リンパ球比率 |
|---|
| Research Abstract |
To investigate aging of human hematopoietic stem cells, we evaluated the expression levels The median age of the donors was 21 y(range 0.2-44y). The purity of CD34-positive cells was greater than 90%. Both target genes were quantitatively detected in all samples. There was an inverse correlation between the expression level of ikzf1 and the donor's age(r=-0.358, p=0.032). In contrast, there was no correlation between the expression level of cebpa and the donor's age. From the data, the mRNA level of ikzf1 in purified CD34-positive cells may reflect the age of HSCs in human bone marrow.
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