Analysis of mechanisms of chronic GVHD post allo-SCT by marking with multicolor florescent proteins

• Project/Area Number: 21591379
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Pediatrics
• Research Institution: National Research Institute for Child Health and Development
• Principal Investigator: ONODERA Masafumi 独立行政法人国立成育医療研究センター, 成育遺伝研究部, 部長 (10334062)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 小児血液学 / 造血幹細胞移植 / 移植片対宿主病 / 蛍光色素 / レトロウイルスベクター

Research Abstract

The purpose of this study is to elucidate the mutual relations between immunocompetent cells such as T cells and antigen presenting cells until the GvHD onset after transplant using mice that GvHD develop due to difference of miHA. To address this issue, I have made virus vectors used in the study and performed transplants between mice with only different miHA(B6 and C3H) and analyzed mechanisms of acute or chronic GvHD.

Research Products

• [Journal Article] Time-lapse imaging reveals symmetric division of GFAP-expressing progenitors for expansion of postnatal dentate granule neurons (2011)
  • Author(s): Namba T, Mochizuki H, Suzuki R, Onodera M, Yamaguchi M, Namiki H, Shioda S, Seki T
  • Journal Title: PLoS ONE Volume: 6 Pages: 25303-25303
  • Peer Reviewed
• [Journal Article] Growth promotion of genetically modified hematopoietic progenitors using antibody/c-Mpl chimera (2011)
  • Author(s): Kawahara M, Chen J, Sogo T, Teng J, Otsu M, Onodera M, Nakauchi H, Ueda H, Nagamune T
  • Journal Title: Cytokine Volume: 55 Pages: 402-408
  • Peer Reviewed
• [Journal Article] Intracellular estrogen receptor-binding fragment associated antigen 9 exerts in vivo tumor promoting effects via its coiled-coil region (2011)
  • Author(s): Maeyama Y, Otsu M, Kubo S, Yamano T, Iimura Y, Onodera M, Kondo S, Sakiyama Y, Ariga T
  • Journal Title: Int J Oncology Volume: 39 Pages: 41-49
  • Peer Reviewed
• [Journal Article] Enforced ROR(gamma) t expression in haematopoietic stem cells increases regulatory T cell number, which reduces immunoreactivity and attenuates hypersensitivity in vivo (2011)
  • Author(s): Fujisawa Y, Nabekura T, Kawachi Y, Otsuka F, Onodera M
  • Journal Title: Asian Pac J Allergy Immunol Volume: 29 Pages: 86-93
  • Peer Reviewed
• [Journal Article] Heterozygous ITGA2B R995W mutation inducing a constitutive activation of theαIIbβ3 receptor affects proplatelet formation and causes congenital macrothrombocytopenia (2011)
  • Author(s): Kunishima S, Kashiwagi H, Otsu M, Takayam N, Eto K, Onodera M, Miyajima Y, Takamatsu Y, Suzumiya J, Matsubara K, Tomiyama Y, Saito H
  • Journal Title: Blood Volume: 117 Pages: 5479-5484
  • Peer Reviewed
• [Journal Article] Potent In Vitro and In Vivo Antitumor Activity of Sorafenib Against Human Intrahepatic Cholangiocarcinoma Cells (2011)
  • Author(s): Sugiyama H, Onuki K, Ishige K, Baba N, Ueda T, Matsuda S, Takeuchi K, Onodera M, Nakanuma Y, Yamato M, Yamamoto M, Hyodo I, Shoda J
  • Journal Title: J Gastroenterol Volume: 46 Pages: 779789-779789
  • NAID: 10029029679
  • Peer Reviewed
• [Journal Article] Osteomyelitis due to triethoprim/sulfamethoxazole-re sistant Edwardsiella tarda infection in a patient with X-linked chronic granulomatous disease (2011)
  • Author(s): Kawai T, Kusakabe H, Seki A, Kobayashi S, Onodera M
  • Journal Title: Infection Volume: 39 Pages: 171-173
  • Peer Reviewed
• [Journal Article] Material obesity impairs hippocampal BDNF production and spatial learning performance in young mouse offspring (2011)
  • Author(s): Tozuka Y, Kumon M, Wada E, Onodera M, Mochizuki H, Wada K
  • Journal Title: Neurochem Int Volume: 57 Pages: 253-257
  • Peer Reviewed
• [Journal Article] Enforced ROR(gamma)t expression in haematopoietic stem cells increases regulatory T cell number, which reduces immuno reactivity and attenuates hypersensitivity in vivo (2011)
  • Author(s): Fujisawa Y, Nabekura T, Kawachi Y, Otsuka F, Onodera M
  • Journal Title: Asian Pac J Allergy Immunol Volume: 29 Pages: 86-93
  • Peer Reviewed
• [Journal Article] Heterozygous ITGA2B R995W mutation inducing a constitutive activation of the αIIbss3 receptor affects proplatelet formation and causes congenital macrothrombocytopenia (2011)
  • Author(s): Kunishima S, Kashiwagi H, Otsu M, Takayam N, Eto K, Onodera M, Miyajima Y, Takamatsu Y, Suzumiya J, Matsubara K, Tomiyama Y, S Hidehiko
  • Journal Title: Blood Volume: 117 Pages: 5479-5484
  • Peer Reviewed
• [Journal Article] Osteomyelitis due to triethoprim/sulfamethoxazole-resistant Edwardsiella tarda infection in a patient with X-linked chronic granulomatous disease (2011)
  • Author(s): Kawai T, Kusakabe H, Seki A, Kobayashi S, Onodera M
  • Journal Title: Infection Volume: 39 Pages: 171-173
  • Peer Reviewed
• [Journal Article] Transactivation of the dopamine receptor 3 gene by a single provirus integration results in development of B cell lymphoma in transgenic mice generated from retrovirally transduced embryonic stem cells (2010)
  • Author(s): Hirata Y Hamanaka S, Onodera M
  • Journal Title: Blood Volume: 115 Pages: 3930-3938
  • Peer Reviewed
• [Journal Article] Phosphodiesterase III inhibition promotes differentiation and survival of oligodendrocyte progenitors and enhances regeneration of ischemic white matter lesions in the adult mammalian brain (2010)
  • Author(s): Miyamoto N, Tanaka R, Shimura H, Watanabe T, Mori H, Onodera M, Mochizuki H, Hattori N, Urabe T
  • Journal Title: J Cereb Blood FlowMetab Volume: 30 Pages: 299-310
  • Peer Reviewed
• [Journal Article] Material obesity impairs hippocampal BDNF production and spatial learning performance in young mouse offspring. (2010)
  • Author(s): Tozuka Y, Kumon M, Wada E, Onodera M, Mochizuki H, Wada K.
  • Journal Title: Neurochem Int Volume: 57 Pages: 253-257
  • Peer Reviewed
• [Journal Article] Transactivation of the dopamine receptor 3 gene by a single provirus integration results in development of B cell lymphoma in transgenic mice generated from retrovirally transduced embryonic stem cells. (2010)
  • Author(s): Hirata Y, Hamanaka S, Onodera M.
  • Journal Title: Blood Volume: 115 Pages: 3930-3938
  • Peer Reviewed
• [Journal Article] Phosphodiesterase III inhibition promotes differentiation and survival of oligodendrocyte progenitors and enhances regeneration of ischemic white matter lesions in the adult mammalian brain. (2010)
  • Author(s): Miyamoto N, Tanaka R, Shimura H, Watanabe T, Mori H, Onodera M, Mochizuki H, Hattori N, Urabe T.
  • Journal Title: J Cereb Blood FlowMetab Volume: 30 Pages: 299-310
  • Peer Reviewed
• [Journal Article] Phosphodiesterase III inhibition promotes differentiation and survival of oligodendrocyte progenitors and enhances regeneration of ischemic white matter lesions in the adult mammalian brain (2010)
  • Author(s): Miyamoto N
  • Journal Title: J Cereb Blood Flow Metab 30 Pages: 299-310
  • Peer Reviewed
• [Journal Article] Identification of tumor-initiating cells in a highly aggressive brain tumor using promoter activity of nucleostemin (2009)
  • Author(s): Tamase A, Muraguchi T, Naka K, Hoshii T, Ohmura M, Kinoshita M, Tanaka S, Shugo H, Ooshio T, Nakada M, Sawamoto K, Onodera M, Matsumoto K, Oshima M, Asano M, Saya H, Okano H, Suda T, Hamada J, Hirao A
  • Journal Title: Proc Natl Acad Sci USA Volume: 106 Pages: 17163-17168
  • Peer Reviewed
• [Journal Article] Gain-of-function of mutated c-Cbl tumour suppressor associated with myeloid neoplasms with 11q UPD (2009)
  • Author(s): Sanada M, Suzuki T, Shih LY, Otsu M, Kato Mo, Yamazaki S, Tamura A, Honda H, Mamiko, Yanagimoto S, Kumano K, Oda H, Yamagata T, Takita Junko, Gotoh N, Nakazaki K, Kawamata N, Onodera M, Nobuyoshi M, Hayashi Y, Harada H, Kurokawa M, Chiba S, Mori1 H, Ozawa K, Omine M, Hirai H, Nakauchi H, Koeffler HP, Ogawa S
  • Journal Title: Nature Volume: 460 Pages: 904-908
  • Peer Reviewed
• [Journal Article] Definiti ve proof for direct reprogramming of hematopoietic cells to pluripotency (2009)
  • Author(s): Okabe M, Otsu M, Ahn DH, Kobayashi T, Morita Y, Wakiyama Y, Onodera M, Eto K, Ema H, Nakauchi H
  • Journal Title: Blood Volume: 114 Pages: 1764-1767
  • Peer Reviewed
• [Journal Article] Crucial role for pCREB signaling in the differentiation and survival of neural progenitors under chronic cerebral hypoperfusion (2009)
  • Author(s): Miyamoto N, Tanaka R, Zhang N, Shimura H, Onodera M, Mochizuki H, Hattori N, Urabe T
  • Journal Title: Neuroscience Volume: 162 Pages: 525-536
  • Peer Reviewed
• [Journal Article] Kinetics and effect of integrin expression in human CD34+cells during MLV-derived retroviral transduction using a recombinant fibronectin for stem cell gene therapy (2009)
  • Author(s): Horiuchi Y, Onodera M, Miyagawa Y, Sato B, Onda K, Katagiri YU, Okita H, Okada M, Otsu M, Kume A, Okuyama T, Fujimoto J, Kuratsuji T, Kiyokawa N
  • Journal Title: Hum Gene Ther Volume: 20 Pages: 777-783
  • Peer Reviewed
• [Journal Article] T cell growth control using hapten-specific antibody/interleukin-2 receptor chimera (2009)
  • Author(s): Sogo T, Kawahara M, Ueda H, Otsu M, Onodera M, Nakauchi H, Nagamune T
  • Journal Title: Cytokine Volume: 46 Pages: 127-136
  • Peer Reviewed
• [Journal Article] The induction of tumor-specific CD4+T cells via major histocompatibility complex class II is required to gain optimal anti-tumor immunity against B16 melanoma cell line in tumor immunotherapy using dendritic cells (2009)
  • Author(s): Fujisawa Y, Nabekura T, Nakao T, Nakamura Y, Takahashi T, Kawachi Y, Otsuka F, Onodera M
  • Journal Title: Exp Dermatol Volume: 18 Pages: 396-403
  • Peer Reviewed
• [Journal Article] Identification of tumor-initiating cells in a highly aggressive brain tumor using promoter activity of nucleostemin (2009)
  • Author(s): Tamase A
  • Journal Title: PNAS 106 Pages: 17163-17168
  • Peer Reviewed
• [Journal Article] Gain-of-function of mutated c-Cbl tumour suppressor5. associated with myeloid neoplasms with 11q UPD (2009)
  • Author(s): Sanada M
  • Journal Title: Nature 460 Pages: 904-908
  • Peer Reviewed
• [Journal Article] Definitive proof for direct reprogramming of hematopoietic cells to pluripotency (2009)
  • Author(s): Okabe M
  • Journal Title: Blood 114 Pages: 1764-1767
  • Peer Reviewed
• [Journal Article] Crucial role for pCREB signaling in the differentiation and survival of neural progenitors under chronic cerebral hypoperfusion (2009)
  • Author(s): Miyamoto N
  • Journal Title: Neuroscience 162 Pages: 525-536
  • Peer Reviewed
• [Journal Article] Kinetics and effect of integrin expression in human CD34+ cells during MLV-derived retroviral transduction using a recombinant fibronectin for stem cell gene therapy (2009)
  • Author(s): Horiuchi Y
  • Journal Title: Hum Gene Ther 20 Pages: 777-783
  • Peer Reviewed
• [Journal Article] T cell growth control using hapten-specific antibody/interleukin-2 receptor chimera (2009)
  • Author(s): Sogo T
  • Journal Title: Cytokine 46 Pages: 127-136
  • Peer Reviewed
• [Journal Article] The induction of tumor-specific CD4+ T cells via major histocompatibility complex class II is required to gain optimal anti-tumor immunity against B16 melanoma cell line in tumor immunotherapy using dendritic cells (2009)
  • Author(s): Fujisawa Y
  • Journal Title: Exp Dermatol 18 Pages: 396-403
  • Peer Reviewed
• [Presentation] 遺伝子治療における我が国と欧米の違い (2011)
  • Author(s): 小野寺雅史
  • Organizer: 第1回国際協力遺伝病遺伝子治療フォーラム
  • Place of Presentation: 東京
• [Presentation] 我が国における遺伝子医薬品承認に向けての課題と取組み (2010)
  • Author(s): 小野寺雅史
  • Organizer: 第8回遺伝子治療シンポジウム
  • Place of Presentation: 大阪
  • Year and Date: 2010-02-05
• [Presentation] 我が国における遺伝子医薬品承認に向けての課題と取組み (2010)
  • Author(s): 小野寺雅史
  • Organizer: 第8回 遺伝子治療シンポジウム
  • Place of Presentation: 大阪
  • Year and Date: 2010-02-05
• [Presentation] An effect of the transcription factor ZFP809 on gene silencing of the retroviral vectors (2010)
  • Author(s): Tadokoro K, Ichida Y, Onodera M
  • Organizer: The 33nd Annual Meeting of the Molecular Biology Society of Japan
  • Place of Presentation: Kobe
• [Presentation] A reciprocal regulation between PAX2 and PAX6 (2009)
  • Author(s): Tadokoro K, Azuma N, Onodera M
  • Organizer: The 32nd Annual Meeting of the Molecular Biology Society of Japan
  • Place of Presentation: Yokohama
  • Year and Date: 2009-12-10
• [Presentation] Kinetics and defect of integrin expression on human CD34+cells during MLV-derived retroviral transduction with a recombinant fibronectin for stem cell gene therapy (2009)
  • Author(s): Horiuchi Y Onodera M, Miyagawa Y, Sato B, Onda K, Katagiri YU, Okita H, Okada M, Otsu M, Kume A, Okuyama T, Fujimoto J, Kuratsuji T, Kiyokawa N
  • Organizer: 第15回日本遺伝子治療学会
  • Place of Presentation: 吹田
  • Year and Date: 2009-07-09
• [Presentation] 我が国における遺伝子医薬品承認に向けての課題と取組み (2009)
  • Author(s): 小野寺雅史
  • Organizer: 第9回小児免疫リウマチ研究会
  • Place of Presentation: 東京
  • Year and Date: 2009-04-10
• [Presentation] 小児難治性疾患に対する遺伝子細胞治療の発展に向けて (2009)
  • Author(s): 小野寺雅史
  • Organizer: 第9回 小児免疫リウマチ研究会
  • Place of Presentation: 東京
  • Year and Date: 2009-04-10
• [Presentation] Kinetics and defect of integrin expression on human CD34+ cells during MLV-derived retroviral transduction with a recombinant fibronectin for stem cell gene therapy (2009)
  • Author(s): Horiuchi Y
  • Organizer: 第15回日本遺伝子治療学会
  • Place of Presentation: 吹田
• [Presentation] A threshold in expression levels of gp91phox transgene limits the degree of functional correction in granulocytes after gene therapy for X-CGD (2009)
  • Author(s): Takeuchi Y
  • Organizer: XVIIth Annual Congress of the European Society of Gene and Cell Therapy
  • Place of Presentation: Hannover, Germany
• [Presentation] Preclinical studies to improve the efficacy of stem cell gene therapy for X-CGD (2009)
  • Author(s): Takeuchi Y
  • Organizer: XVth Annual Meeting of the Japanese Society of Gene Therapy
  • Place of Presentation: Osaka