| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Research Abstract |
Monoclonal antibodies were produced as the influenza C virus-specific antibodies for rapid diagnosis of influenza C virus infection and the real-time PCR assay for influenza C virus was newly developed. Respiratory specimens from children were examined for the presence of influenza C virus by cell culture and real-time PCR. Taking the cell culture results as the gold standard, the sensitivity, specificity, positive predictive value and negative predictive value for the real-time PCR were found to be 100%, 98.5%, 66.7% and 100%, respectively. The mean viral load in specimens that were positive by cell culture was significantly high than those in specimens that were negative by cell culture. The real-time PCR assay established here can be used as a sensitive method for diagnosing influenza C virus infection
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