| Project/Area Number |
21591386
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pediatrics
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| Research Institution | Shiga University of Medical Science |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
FUJINO Hidetoshi 滋賀医科大学, 医学部, 助教 (40209078)
MATSUURA Hiroshi 滋賀医科大学, 医学部, 教授 (60238962)
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| Research Collaborator |
HANATO Takashi 滋賀医科大学, 医学部, 研究生
KISE Kazuyosi 医法社団昴会湖東記念病院, 小児科医長
HOSHINO Shinsuke 滋賀医科大学, 医学部, 大学院生
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 発生・分化 / 細胞・組織 / パッチクランプ法 / イオンチャネル / 静止膜電位 / 生理・機能 / 動物 / マウス / 単離心筋細胞 / Langendorff灌流 / 生理学的特性 / 成長 / パッチクランプ / ラット / 刺激伝導系細胞 / 心筋細胞 / ビスダイアミン / イオンチャンネル / ラット胚 / 心奇形モデル / 発生 / 全胚培養 |
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| Research Abstract |
Bis-diamine induces congenital heart defects in embryos when administered to pregnant rats. Histological analyses of the developing cardiac conduction system were performed in this animal model and the normal control rat embryos using whole embryo culture. Bis-diamine disturbed the conduction tissue development through overall poor myocardial growth. We investigated developmental changes of the physiological characteristics in the neonatal mouse myocardial cells isolated using chunk method or from Langendorff perfusion method. All the isolated cells exhibited elongated spindle-shaped appearance and a conduction tissue cell could not be distinguished from a myocardial cell or a fibroblast. Whole-cell patch-clamp experiments clearly showed the developmental changes in electrophysiological characteristics of the myocardial cells including the decrease in the inward rectifier K^+current and depolarization of resting membrane potential.
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