| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Research Abstract |
We identified transport protein x bounded to podocin, important molecule for proteinuria, with immunoprecipitation and LC/MS. The double staining with endothelial marker and anti-x antibody indicated that protein x was expressed in podocyte. When protein x was suppressed with siRNA in cells, podocin expression along cell membrane was much lower than control and cell shape was changed. Overexpression of protein x improved expression of mutated podocin along cell membrane. These data indicated protein x play role in membrane trafficking of podocin.
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