A study of avidity of Haemophilus influenzae type b (Hib)antibody and multiple copies of capsulation loci in the strains as possible factors of Hib vaccine failure in Japan.

• Project/Area Number: 21591390
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Pediatrics
• Research Institution: Tohoku University (2010-2011); Kagoshima University (2009)
• Principal Investigator: TOKUDA Koichi 東北大学, 病院, 講師 (10518400)
• Co-Investigator(Kenkyū-buntansha): NISHI Junichiro 鹿児島大学, 医学部・歯学部付属病院, 講師 (40295241)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: ヒブワクチン / vaccine failure / Hib莢膜遺伝子capb領域の重複 / Hib抗体のavidity低下

Research Abstract

Haemophilus influenzae type b (Hib)occasionally causes invasive bacterial diseases such as meningitis especially among young children. In recent years, vaccine failure associated with multiple amplification of the capsulation(capb)locus has been increasingly reported in Europe. We examined Hib strains from Japanese children with Hib meningitis and founded that the proportion of strains with three or four copies of the capb locus already exist before the popularization of Hib vaccination.

Research Products

• [Journal Article] Quantitative analysis of biofilm formation of methicillin-resistant Staphylococcus aureus(MRSA)strains from patients with orthopaedic device-related infections (2011)
  • Author(s): Kawamura H, Nishi J, Imuta N, Tokuda K, Miyanohara H, Hashiguchi T, Zenmyo M, Yamamoto T, Ijiri K, Kawano Y, and Komiya S
  • Journal Title: FEMS Immunology & Medical Microbiology Volume: 63 Pages: 10-15
  • Peer Reviewed
• [Journal Article] Quantitative analysis of biofilm formation of methicillin-resistant Staphylococcus aureus (MESA) strains from patients with orthopaedic device-related infections (2011)
  • Author(s): Kawamura H, Nishi J, Imuta N, Tokuda K, Miyanohara H, Hashiguchi T, Zenmyo M, Yamamoto T, Ijiri K, Kawano Y, Komiya S
  • Journal Title: FEMS Immunology & Medical Microbiology Volume: 63 Issue: 1 Pages: 10-15
  • DOI: 10.1111/j.1574-695x.2011.00821.x
  • Peer Reviewed
• [Journal Article] Presence of multiple copies of capsulation loci in invasive Haemophilus influenzae type b(Hib)strains in Japan before introduction of the Hib conjugate vaccine (2010)
  • Author(s): Ueno K, Nishi J, Imuta N, Tokuda K, Kawano Y
  • Journal Title: Microbiol Immunol Volume: 54 Pages: 160-163
  • NAID: 10029544305
  • Peer Reviewed
• [Journal Article] Characterization of typical and atypical enteroaggregative escherichia coli in Kagoshima, Japan : biofilm formation and acid resistance. (2010)
  • Author(s): Koichi Tokuda, Junichiro Nishi, Naoko Imuta, Rika Fujiyama, Akira Kamenosono, Kunihiro Manago, Yoshifumi Kawano
  • Journal Title: Microbiology and Immunology Volume: 54 Pages: 160-163
  • NAID: 10029545048
  • Peer Reviewed
• [Journal Article] Presence of multiple copies of capsulation loci in invasive Haemophilus influenzae type b(Hib)strains in Japan before introduction of the Hib conjugate vaccine (2010)
  • Author(s): Kentaro Ueno, Junichiro Nishi, Naoko Imuta, Koichi Tokuda, Yoshifumi Kawano
  • Journal Title: Microbiology and Immunology 54 Pages: 160-163
  • NAID: 10029544305
  • Peer Reviewed
• [Presentation] Detection and Control of a Pertussis Outbreak in a Medical School and University Hospital (2012)
  • Author(s): Tokuda Koichi
  • Organizer: The 2012 International Conference on Emerging Infectious Diseases
  • Place of Presentation: Atlanta, USA
  • Year and Date: 2012-05-12
• [Presentation] Detection and Control of a Pertussis Outbreak in a Medical School and University Hospital (2012)
  • Author(s): Tokuda Koichi
  • Organizer: The 2012 International Conference on Emerging Infectious Diseases
  • Place of Presentation: the Hyatt Regency Atlanta(米国)
  • Year and Date: 2012-03-12
• [Presentation] 腸管出血性大腸菌サーベイランスにおける地理情報システム(GIS)を用いた効果的情報還元に関する研究 (2011)
  • Author(s): 徳田浩一
  • Organizer: 第43回日本小児感染症学会総会・学術集会
  • Place of Presentation: 岡山コンベンションセンター(岡山県)
  • Year and Date: 2011-10-29
• [Presentation] 小児下痢症患児から分離された大腸菌におけるESBL(基質拡張型βラクタマーゼ)遺伝子の保有状況 (2011)
  • Author(s): 西順一郎
  • Organizer: 第43回日本小児感染症学会総会・学術集会
  • Place of Presentation: 岡山コンベンションセンター(岡山県)
  • Year and Date: 2011-10-29
• [Presentation] Assessment of sanitary and infectious risk factors in evacuation centers after the great east Japan earthquake (2011)
  • Author(s): Tokuda Koichi
  • Organizer: The 49th Annual Meeting of the Infectious Diseases Society of America
  • Place of Presentation: Boston, USA
  • Year and Date: 2011-10-21
• [Presentation] Assessment of sanitary and infectious risk factors in evacuation centers after the great east Japan earthquake (2011)
  • Author(s): Tokuda Koichi
  • Organizer: The 49th Annual Meeting of the Infectious Diseases Society of America
  • Place of Presentation: Boston Exhibition and Convention Center(米国)
  • Year and Date: 2011-10-21
• [Presentation] 糖鎖固定化金ナノ粒子(SGNP)RT-qPCR法による患者唾液中のインフルエンザウイルス遺伝子の検出 (2011)
  • Author(s): 西順一郎
  • Organizer: 第44回日本小児呼吸器疾患学会
  • Place of Presentation: ホテル東日本宇都宮(栃木県)
  • Year and Date: 2011-10-16
• [Presentation] 腸管出血性大腸菌感染症の報告促進における課題-医師側要因に関するKAP調査- (2010)
  • Author(s): 徳田浩一
  • Organizer: 第42回日本小児感染症学会総会・学術集会
  • Place of Presentation: 仙台国際センター(宮城県)
  • Year and Date: 2010-11-27
• [Presentation] 鹿児島県における小児細菌性髄膜炎の前方視的調査とHibワクチン安全性調査 (2010)
  • Author(s): 西順一郎
  • Organizer: 第42回日本小児感染症学会総会・学術集会
  • Place of Presentation: 仙台国際センター(宮城県)
  • Year and Date: 2010-11-27
• [Presentation] collagen adhesin gene(cna)を保有するMRSAの biofilm 形成能の検討 (2010)
  • Author(s): 藺牟田直子, 川村英樹, 徳田浩一, 玉井真理子, 西順一郎
  • Organizer: 第83回日本細菌学会総会
  • Place of Presentation: 神奈川県
  • Year and Date: 2010-03-27
• [Presentation] 鹿児島県におけるヒブワクチン安全性の前方視的調査 (2009)
  • Author(s): 西順一郎, 徳田浩一, 藺牟田直子, 河野嘉文
  • Organizer: 第41回日本小児感染症学会総会・学術集会
  • Place of Presentation: 福井県
  • Year and Date: 2009-11-14
• [Presentation] 整形外科患者の鼻腔および感染創由来MRSA株の遺伝子型、バイオフィルム形成能の検討 (2009)
  • Author(s): 川村英樹, 西順一郎, 藺牟田直子, 徳田浩一, 宮之原弘晃
  • Organizer: 第83回日本感染症学会総会
  • Place of Presentation: 東京都
  • Year and Date: 2009-04-24