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Proteomic analysis of ductus arteriosus by two-dimensional difference gel electrophoresis

Research Project

Project/Area Number 21591399
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Pediatrics
Research InstitutionTokyo Women's Medical University

Principal Investigator

HAYAMA Emiko  東京女子医科大学, 医学部, 非常勤講師 (00349698)

Co-Investigator(Kenkyū-buntansha) NAKANISHI Toshio  東京女子医科大学, 医学部, 教授 (90120013)
Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywordsタンパク質 / シグナル伝達 / 動脈管 / 酸素感受性 / プロテオミクス / シグナル伝逹
Research Abstract

Increased PO2 contracts ductus arteriosus(DA) and relaxes pulmonary artery(PA). One of up-regulated proteins in DA under oxygenated condition was determined as peroxiredoxin 4, which regulates NF-kB activity via a modulation of I kB-a phosphorylation. Up-regulation of peroxiredoxin 4 in DA under oxygenated condition may contribute to the constriction of ductus arteriosus in response to change in the PO2 level after birth. Functional DA closure depends on gestational maturity. One of up-regulated proteins in the term DA was determined as reticulocalbin 3.Another identified up-regulated protein was tropomyosin 2 involved in muscle contraction. These proteins may accelerate migration of the smooth muscle cells from media to subendothelial space in the term DA, which contributes anatomical closure of the DA.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (26 results)

All 2012 2011 2010 2009

All Journal Article (9 results) (of which Peer Reviewed: 6 results) Presentation (17 results)

  • [Journal Article] 胎児血管の収縮制御システムの発現変動解析2012

    • Author(s)
      中西敏雄, 羽山恵美子, 勝部康弘
    • Journal Title

      (財)日本心臓血圧研究振興会(平成二十三年度研究業績集)

      Volume: 26

    • Related Report
      2011 Final Research Report
  • [Journal Article] 胎生期の動脈管を始めとした心血管におけるタンパク質発現の解析2011

    • Author(s)
      中西敏雄, 羽山恵美子, 勝部康弘
    • Journal Title

      (財)日本心臓血圧研究振興会(平成二十二年度研究業績集)

      Volume: 25 Pages: 17-21

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Journal Article] In vivo dilatation of the ductus arteriosus induced by furosemide in the rat2010

    • Author(s)
      Toyoshima K, Momma K, Nakanishi T.
    • Journal Title

      Pediatric Research

      Volume: 67 Pages: 173-176

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] The role of the large-conductance voltage-dependent and calcium-activated potassium(BK(Ca)) channels in the regulation of rat ductus arteriosus tone2010

    • Author(s)
      Sun F, Hayama E, Katsube Y, Matsuoka R, Nakanishi T.
    • Journal Title

      Heart & Vessels

      Volume: 25 Pages: 556-564

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] 肺動脈と動脈管における蛍光標識二次元電気泳動法を用いた酸素感受性蛋白質の発現変動解析2010

    • Author(s)
      中西敏雄, 羽山恵美子, 松岡瑠美子, 勝部康弘
    • Journal Title

      (財)日本心臓血圧研究振興会(平成二十一年度研究業績集)

      Volume: 24 Pages: 13-17

    • Related Report
      2011 Final Research Report
  • [Journal Article] Delayed neotnatal closure of the ductus arteriosus following early in utero exposure to indomethacin in the rat2010

    • Author(s)
      Kazuo Momma, Katsuaki Toyoshima, Kiyomi Ito, Kiyoshi Sugiyama, Shinichiro Imamura, Fang Sun, Toshio Nakanishi
    • Journal Title

      Neonatology

      Volume: 96 Pages: 69-79

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] The role of the large-conductance voltage-dependent and calcium-activated potassium(BK(Ca))channels in the regulation of rat ductus arteriosus tone.2010

    • Author(s)
      Sun F, Hayama E, Katsube Y, Matsuoka R, Nakanishi T.
    • Journal Title

      Heart & Vessels

      Volume: 25 Pages: 556-564

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] In vivo dilatation of the ductus arteriosus induced by furosemide in the rat.2010

    • Author(s)
      Toyoshima K, Momma K, Nakanishi T.
    • Journal Title

      Pediatric Research

      Volume: 67 Pages: 173-176

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Delayed neotnatal closure of the ductus arteriosus following early in utero exposure to indomethacin in the rat.2009

    • Author(s)
      Kazuo Momma, Katsuaki Toyoshima, Kiyomi Ito, Kiyoshi Sugiyama, Shinichiro Imamura, Fang Sun, Toshio Nakanishi
    • Journal Title

      Neonatology 96

      Pages: 69-79

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] 胎仔発達に伴う肺動脈・動脈管の発現タンパク質の変化2012

    • Author(s)
      羽山恵美子, 中西敏雄
    • Organizer
      第18回日本小児肺循環研究会
    • Place of Presentation
      東京
    • Year and Date
      2012-02-04
    • Related Report
      2011 Final Research Report
  • [Presentation] 胎仔発達に伴う肺動脈・動脈管の発現タンパク質の変化2012

    • Author(s)
      羽山恵美子, 中西敏雄
    • Organizer
      第18回日本小児肺循環研究会
    • Place of Presentation
      笹川記念会館(東京)
    • Year and Date
      2012-02-04
    • Related Report
      2011 Annual Research Report
  • [Presentation] Developmental Difference in Maturation of Sarcoplasmic Reticulum in the Large Blood Vessels Influences Their Contractility2011

    • Author(s)
      羽山恵美子, 中西敏雄
    • Organizer
      American Heart Association Scientific Session
    • Place of Presentation
      フロリダ,アメリカ合衆国
    • Year and Date
      2011-11-07
    • Related Report
      2011 Final Research Report
  • [Presentation] 膜電位依存性カリウムチャネル1.5の遺伝子多型における機能変化の検討2011

    • Author(s)
      羽山恵美子, 勝部康弘, 中西敏雄
    • Organizer
      第47回日本小児循環器学会
    • Place of Presentation
      福岡
    • Year and Date
      2011-07-07
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] Developmental Difference in Maturation of Sarcoplasmic Reticulum in the Large Blood Vessels Influences Their Contractility2011

    • Author(s)
      羽山恵美子, 中西敏雄
    • Organizer
      American Heart Association Scientific Session 2011
    • Place of Presentation
      アメリカ,フロリダ
    • Year and Date
      2011-07-07
    • Related Report
      2011 Annual Research Report
  • [Presentation] 肺動脈・動脈管の発達に伴う収縮制御に関する遺伝子群の発現変動2011

    • Author(s)
      羽山恵美子, 中西敏雄
    • Organizer
      第17回日本小児肺循環研究会
    • Place of Presentation
      東京
    • Year and Date
      2011-02-05
    • Related Report
      2011 Final Research Report
  • [Presentation] 肺動脈・動脈管の発達に伴う収縮制御に関する遺伝子群の発現変動2011

    • Author(s)
      羽山恵美子, 中西敏雄
    • Organizer
      第16回日本小児肺循環研究会
    • Place of Presentation
      笹川記念会館(東京都)
    • Year and Date
      2011-02-05
    • Related Report
      2010 Annual Research Report
  • [Presentation] Naturally Occurring L138Q Mutation in the Voltage-gated Potassium Channel 1.5 Impairs the Channel Activity2011

    • Author(s)
      羽山恵美子, 勝部康弘, 中西敏雄
    • Organizer
      第75回日本循環器学会
    • Place of Presentation
      横浜
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] 蛍光標識二次元電気泳動法を用いた肺動脈・動脈管酸素感受性蛋白質の検討2010

    • Author(s)
      羽山恵美子, 中西敏雄
    • Organizer
      第46回日本小児循環器学会
    • Place of Presentation
      東京
    • Year and Date
      2010-07-08
    • Related Report
      2011 Final Research Report
  • [Presentation] 蛍光標識二次元電気泳動法を用いた肺動脈-動脈管酸素感受惇蛋白質の検討2010

    • Author(s)
      羽山恵美子, 中西敏雄
    • Organizer
      第46回日本小児循環器学会
    • Place of Presentation
      シェラトン・グランデ・トーキョーベイ・ホテル(東京都)
    • Year and Date
      2010-07-08
    • Related Report
      2010 Annual Research Report
  • [Presentation] 蛍光標識二次元電気泳動法を用いた肺動脈・動脈管酸素感受性蛋白質の検索2010

    • Author(s)
      羽山恵美子, 中西敏雄
    • Organizer
      第16回日本小児肺循環研究会
    • Place of Presentation
      東京
    • Year and Date
      2010-02-06
    • Related Report
      2011 Final Research Report
  • [Presentation] 蛍光標識二次元電気泳動法を用いた肺動脈・動脈管酸素感受性蛋白質の検索2009

    • Author(s)
      羽山恵美子、中西敏雄
    • Organizer
      第16回日本小児肺循環研究会
    • Place of Presentation
      東京
    • Year and Date
      2009-02-06
    • Related Report
      2009 Annual Research Report
  • [Presentation] 膜電位依存性カリウムチャネル1.5のglycosylationに関する検討2009

    • Author(s)
      羽山恵美子, 中西敏雄
    • Organizer
      第82回日本生化学会大会合同大会
    • Place of Presentation
      神戸
    • Related Report
      2011 Final Research Report 2009 Annual Research Report
  • [Presentation] ブタ新生仔大血管膜電位依存性カリウムチャンネル複合体の分離2009

    • Author(s)
      羽山恵美子, 中西敏雄
    • Organizer
      第45回日本小児循環器学会
    • Place of Presentation
      神戸
    • Related Report
      2011 Final Research Report 2009 Annual Research Report
  • [Presentation] 家兎血管平滑筋における酸素感受性蛋白質-iTRAQ2D-LC-MS/MS法による解析-2009

    • Author(s)
      勝部康弘、孫芳、羽山恵美子、中西敏雄、小川俊一
    • Organizer
      第45回日本小児循環器学会
    • Place of Presentation
      神戸
    • Related Report
      2011 Final Research Report
  • [Presentation] 先天性心疾患の拡張した大動脈ではTGF-betaが発現している2009

    • Author(s)
      孫芳, 羽山恵美子, 中西敏雄
    • Organizer
      第45回日本小児循環器学会
    • Place of Presentation
      神戸
    • Related Report
      2011 Final Research Report 2009 Annual Research Report
  • [Presentation] 家兎血管平滑筋における酸素感受性蛋白質 - iTRAQ2D-LC-MS/MS法による解析 -2009

    • Author(s)
      勝部康弘、孫芳、羽山恵美子、中西敏雄、小川俊一
    • Organizer
      第45回日本小児循環器学会
    • Place of Presentation
      神戸
    • Related Report
      2009 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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