Application of SELDI ProteinChip system in pathogenesis of pediatric fulminamt hepatic failure

• Project/Area Number: 21591403
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Pediatrics
• Research Institution: National Research Institute for Child Health and Development
• Principal Investigator: KASAHARA Mureo 独立行政法人国立成育医療研究センター, 臓器移植センター, センター長 (30324651)
• Co-Investigator(Kenkyū-buntansha): MATSUI Akira 独立行政法人国立成育医療研究センター, 院長 (00159146) ; NAKAZAWA Atsuko (NAKAGAWA Atsuko) 独立行政法人国立成育医療研究センター, 病理診断部, 部長 (90227736) ; YOSHIKAWA Kazuhiro 愛知医科大学, 医学部, 准教授 (60109759)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2009: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 劇症肝不全 / サイトカイン / プロテオーム

Research Abstract

We examined about cytokine expression, infiltrating lymphocytes, protein expression in explanted liver tissue of pediatric fulminant hepatic failure(FHF). Five cytokines which were expressed higher in FHF liver tissue were identified by cytokine array(RayBio). Cytokine A which showed highest expression was localized in sinus endothelial cells, and the receptor was seen in hepatocytes and ductular cells by immunohistochemistry. Infiltrating lymphocytes were predominantly cCD3+CD8+granzymeB+T-cell and their distribution in sinus was characteristic. Using SELDI ProteinChip system, three highly expressed molecules and three lowly expressed molecules were identified by CM10 Chip, and one highly and one lowly expressed molecule were identified by Q10 Chip. Further analysis of those molecules could promise to clarify the pathogenesis of pediatric FHF.

Research Products

• [Journal Article] Living-donor liver trans-plantation using hyper-reduced graft for a neonatal fulminant hepatic failure (2011)
  • Author(s): Sasaki K, Kasahara M, Fukuda A, Kimura T, Shigeta T, Tanaka H, Nakagawa S, Ito S, Nakagawa A.
  • Journal Title: Pediatr Int Volume: 53 Pages: 247-248
  • Peer Reviewed
• [Journal Article] Living-donor liver transplantation using hyper-reduced graft for a neonatal fulminant hepatic failure (2011)
  • Author(s): Sasaki K, Kasahara M, Fukuda A, Kimura T, Shigeta T, Tanaka H, Nakagawa S, Ito S, Nakagawa A
  • Journal Title: Pediatr Int Volume: 53 Pages: 247-248
  • NAID: 10029557986
  • Peer Reviewed
• [Journal Article] Living-donor liver transplantation for propionic acibemia (2011)
  • Author(s): Kasahara M, Sakamoto S, Kanazawa H, Karaki C, Kakiuchi T, Shigeta T, Fukuda A, Kosaki R, Nakazawa A, Ishige M, Nagao M, Shigematsu Y, Yorifuji T, Naiki M, Nagao M, Shigematsu Y, Yorifuji T, Naiki Y, Horikawa R
  • Journal Title: Pediatr Transplant Volume: (2011 Dec 7.(Epub ahead of print))
  • Peer Reviewed
• [Journal Article] 胆汁うっ滞症に対する肝移植の問題点 (2011)
  • Author(s): 笠原群生, 垣内俊彦, 阪本清介, 福田晃也, 重田孝信, 中澤温子, 松井陽
  • Journal Title: 小児内科 Volume: 43 Pages: 1077-1081
• [Journal Article] Epstein-barr virus infection after pediatric living-related liver transplantation-management and risk factors (2010)
  • Author(s): Shigeta T, Imadome K, Sakamoto S, Fukuda A, Kakiuchi T, Matsuno N, Tanaka H, Nakazawa A, Kasahara M
  • Journal Title: Transplant Proc Volume: 42(10) Pages: 4178-4180
  • Peer Reviewed
• [Journal Article] Living Donor Liver Transplantation for Carbamoyl Phosphate Synthetase 1 Deficiency. (2010)
  • Author(s): Kasahara M, Sakamoto S, Shiegta T, Fukuda A, Kozaki R, Nakazawa A, Uemoto S, Noda M, Naiki Y, Horikawa R
  • Journal Title: Pediatr Transplantation Volume: 14 Pages: 1036-1040
  • Peer Reviewed
• [Journal Article] Epstein-barr virus infection after pediatric living-related liver transplantation-management and risk factors. (2010)
  • Author(s): Shigeta T, Imadome K, Sakamoto S, Fukuda A, Kakiuchi T, Matsuno N, Tanaka H, Nakazawa A, Kasahara M
  • Journal Title: Transplant Proc. Volume: 42(10) Pages: 4178-4180
  • Peer Reviewed
• [Journal Article] 当院における小児劇症肝不全24症例に対する人工肝補助の経験 (2010)
  • Author(s): 堤晶子, 小椋雅夫, 宇田川智宏, 野田俊輔, 亀井宏一, 唐木千晶, 中川聡, 福田晃也, 笠原群生, 中川温子, 伊藤秀一
  • Journal Title: 日本小児腎不全学会雑誌 Volume: 30 Pages: 304-306
  • NAID: 10027698122
  • Peer Reviewed
• [Journal Article] 小児劇症肝不全の集学的管理と生体肝移植適応 (2009)
  • Author(s): 唐木千晶,笠原群生,清水直樹,六車崇,齊藤修,齊藤一郎,中川聡
  • Journal Title: 日本集中治療医学会雑誌 Volume: 16巻3号 Pages: 279-288
  • NAID: 10025572947
  • Peer Reviewed
• [Journal Article] 当院における小児劇症肝不全9症例に対する血液浄化療法/人工肝補助の経験 (2009)
  • Author(s): 白川利彦,亀井宏一,寺町昌史,笠原克明,中山真紀子,鈴木輝明,肥沼幸,新井勝大,清水直樹,中川聡,笠原群生,飯島一誠
  • Journal Title: 日本小児腎不全学会雑誌 Volume: 28巻 Pages: 202-204
  • NAID: 10025701802
  • Peer Reviewed
• [Journal Article] Epstein-Barr virus-associated erythema nodosum after living-donor liver transplant-a case report (2009)
  • Author(s): Yokoyama S, Kasahara M, Fukuda A, Sato S, Koda F, Nakagawa A.
  • Journal Title: Liver Transpl 15(4) Pages: 446-448
  • Peer Reviewed
• [Journal Article] Liver transplantation in a patient with propionic academia requiring extra corporeal membrane oxygenation during severe metabolic decompensation (2009)
  • Author(s): Sato S, Kasahara M, Fukuda A, Mizuguchi K, Nakagawa S, Muguruma T, Saito O, Karaki C, Nakagawa A, Yoshii K, Horikawa R.
  • Journal Title: Pediatr Transplant 13(6) Pages: 790-793
  • Peer Reviewed
• [Journal Article] Living donor liver transplantation for hepatoblastoma with Beckwith-Wiedemann syndrome (2009)
  • Author(s): Sasaki K, Kasahara M, Fukuda A, Shigeta T, Tanaka H, Nakagawa S, Mitsui K, Harada R, Nakagawa A.
  • Journal Title: Pediatr Transplant (Epub ahead of print)
  • Peer Reviewed
• [Journal Article] Liver transplantation for an infant with neonatal intrahepatic cholestasis caused by citrin deficiency using heterozygote living donor (2009)
  • Author(s): Shigeta T, Kasahara M, Kimura T, Fukuda A, Sasaki K, Arai K, Nakagawa A, Nakagawa S, Kobayashii K, Soneda S, Kitagawa H.
  • Journal Title: Pediatr Transplant (Epub ahead of print)
  • Peer Reviewed
• [Journal Article] Living Donor Liver Transplantation for a Congenital Absence of the Portal Vein with Situs Inversus (2009)
  • Author(s): Kasahara M, Nakagawa A, Sakamoto S, Tanaka H, Shigeta T, Fukuda A, Nosaka S, Matsui A.
  • Journal Title: Liver Transpl 15(11) Pages: 1641-1643
  • Peer Reviewed
• [Journal Article] Living Donor Liver Transplantation for Glycogen Storage Disease Type Ib (2009)
  • Author(s): Kasahara M, Horikawa R, Sakamoto S, Shigeta T, Tanaka H, Fukuda A, Abe K, Yoshii K, Naiki Y, Kosaki R, Nakagawa A.
  • Journal Title: Liver Transpl 15(12) Pages: 1867-1871
  • Peer Reviewed
• [Presentation] Pediatric fulminant hepatic failure of unknown etiology-A unique immune mediated mechanism of CD8-positive T-cell activation causing simultaneous bone marrow suppression (2012)
  • Author(s): Nakazawa A, Irie R, Matsuoka K, Sakamoto S, Kasahara M.
  • Organizer: The 101^<st> USCAP annual meeting Vancouver
  • Place of Presentation: Canada
  • Year and Date: 2012-03-21
• [Presentation] Pediatric fulminant hepatic failure of unknown etiology-A unique immune mediated mechanism of CD8-positive T-cell activation causing simultaneous bone marrow suppression (2012)
  • Author(s): Nakazawa A, Irie R, Matsuoka K, Sakamoto S, Kasahara M
  • Organizer: The 101^<st> USCAP annual meeting
  • Place of Presentation: Vancouver, Canada
  • Year and Date: 2012-03-21
• [Presentation] 小児特発性劇症肝不全の病態に関する検討 (2011)
  • Author(s): 入江理恵,中澤温子,佐藤泰樹,中野夏子,大喜多肇,松岡健太郎,垣内俊彦,福田晃也,阪本靖介,笠原群生
  • Organizer: 第39回日本肝臓学会西部会
  • Place of Presentation: 岡山
  • Year and Date: 2011-12-10
• [Presentation] 小児原因不明劇症肝不全の臨床病理学的特徴 (2011)
  • Author(s): 中澤温子,入江理恵,佐藤泰樹,中野夏子,大喜多肇,松岡健太郎,垣内俊彦,福田晃也,阪本靖介,笠原群生
  • Organizer: 第39回日本肝臓学会西部会
  • Place of Presentation: 岡山
  • Year and Date: 2011-12-10
• [Presentation] 当センターにおける小児急性肝不全症例に対する肝移植医療体制の検討 (2011)
  • Author(s): 垣内俊彦,阪本靖介,金澤寛之,唐木千晶,福田晃也,中川聡,中澤温子,笠原群生,松井陽
  • Organizer: 第47回日本移植学会総会
  • Place of Presentation: 仙台
  • Year and Date: 2011-10-06
• [Presentation] 小児劇症肝不全に対する生体肝移植後にbasiliximabを難治性拒絶反応のRescue療法として使用した4例の検討 (2011)
  • Author(s): 福田晃也, 唐木千晶, 垣内俊彦, 金澤寛之, 田中秀明, 河合利尚, 阪本靖介, 今留謙一, 中澤温子, 松野直徒, 笠原群生
  • Organizer: 第47回日本移植学会総会
  • Place of Presentation: 仙台
  • Year and Date: 2011-10-06
• [Presentation] 小児特発性劇症肝不全の病態に関する検討 (2011)
  • Author(s): 入江理恵,中澤温子,中野夏子,松岡健太郎
  • Organizer: 第100回日本病理学会総会
  • Place of Presentation: 横浜
  • Year and Date: 2011-04-29
• [Presentation] 小児劇症肝不全に対する生体肝移植血液学的推移と合併症の報告 (2010)
  • Author(s): 木澤洋恵,堀越麻玲,小川礼佳,野中裕子,安齋利一,石井幸雄,宮崎澄夫,笠原群生,森鉄也,中澤温子
  • Organizer: 第57回日本臨床検査医学会学術集会
  • Place of Presentation: 東京
  • Year and Date: 2010-09-10
• [Presentation] 小児劇症肝不全に対する生体肝移植 血液学的推移と合併症の報告 (2010)
  • Author(s): 木澤洋恵, 堀越麻玲, 小川礼佳, 野中裕子, 安齋利一, 石井幸雄, 宮崎澄夫, 笠原群生, 森鉄也, 中澤温子
  • Organizer: 第57回日本臨床検査医学会学術集会
  • Place of Presentation: 東京
  • Year and Date: 2010-09-10
• [Presentation] 肝不全を呈し、致死的経過を辿ったMitochondria)DNA Depletion Syndromesの1例 (2010)
  • Author(s): 垣内俊彦, 肥沼幸, 柳忠宏, 清水泰岳, 新井勝大, 中川聡, 中澤温子, 笠原群生, 村山圭, 松井陽
  • Organizer: 第27回日本小児肝臓研究会
  • Place of Presentation: 千葉
  • Year and Date: 2010-07-25
• [Presentation] 重症肝不全を合併した全身型若年性黄色肉芽腫(disseminated juvenile xantho-granuloma)の1例 (2010)
  • Author(s): 垣内俊彦,柳忠宏,清水泰岳,新井勝大,中川聡,中澤温子,笠原群生,斉藤朋子,松井陽
  • Organizer: 第27回日本小児肝臓研究会
  • Place of Presentation: 千葉
  • Year and Date: 2010-07-24
• [Presentation] 重症肝不全を合併した全身型若年性黄色肉芽腫(disseminated juvenile xanthogranuloma)の1例 (2010)
  • Author(s): 垣内俊彦, 柳忠宏, 清水泰岳, 新井勝大, 中川聡, 中澤温子, 笠原群生, 斉藤朋子, 松井陽
  • Organizer: 第27回日本小児肝臓研究会
  • Place of Presentation: 千葉
  • Year and Date: 2010-07-24
• [Presentation] Long-term outcome and new indications of pediatric living donor liver transplantation (2010)
  • Author(s): 笠原群生
  • Organizer: 2010 Liver Transplantation Seminer
  • Place of Presentation: Seoul, Korea
  • Year and Date: 2010-03-12
• [Presentation] Surgical technique in pediatric liver transplantation (2010)
  • Author(s): 笠原群生
  • Organizer: Liver Transplantation Seminer
  • Place of Presentation: Seoul, Korea
  • Year and Date: 2010-03-12
• [Presentation] 当院における、肝移植後CMV・EBV感染の現況と対策 (2010)
  • Author(s): 重田孝信,阪本靖介,福田晃也,田中秀明,中川温子,笠原群生
  • Organizer: 第28回日本肝移植研究会
  • Place of Presentation: 広島
• [Presentation] 当院における、肝移植後CMV-EBV感染の現況と対策 (2010)
  • Author(s): 重田孝信, 阪本靖介, 福田晃也, 田中秀明, 中川温子, 笠原群生
  • Organizer: 第28回日本肝移植研究会
  • Place of Presentation: 広島
• [Presentation] 肝移植後の免疫抑制剤の合併症・対策について (2009)
  • Author(s): 笠原群生
  • Organizer: 27回大日本肝移植研究会
  • Place of Presentation: 静岡
  • Year and Date: 2009-07-10
• [Presentation] Living-donor liver transplantation for Methylmalonic Acidemia(MMA); Is it a curative operation (2009)
  • Author(s): Kasahara M, Sakamoto S, Fukuda A, Shigeta T, Nakazawa A.
  • Organizer: 5^<th> International Pediatric Trasnplant Association
  • Place of Presentation: Istanbul, Turkey
  • Year and Date: 2009-04-20
• [Remarks]
  • URL: http://www.ncchd.go.jp/hospital/section/special/transplant_surgery/fulminant_hepatitis.html