Analysis of Epstein-Barr virus infection following pediatric liver transplantation by molecular EBV monitoring

• Project/Area Number: 21591404
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Pediatrics
• Research Institution: National Research Institute for Child Health and Development
• Principal Investigator: FUKUDA Akinari 独立行政法人国立成育医療研究センター, 移植外科, 医員 (60455417)
• Co-Investigator(Kenkyū-buntansha): IMADOME Ken-ichi 独立行政法人国立成育医療研究センター, 母児感染研究部, 室長 (70392488) ; UMEZAWA Akihiro 独立行政法人国立成育医療研究センター, 生殖・細胞医療研究部, 部長 (70213486)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2009: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
• Keywords: 小児感染症学 / 小児生体肝移植 / EBウイルス / EBV / PTLD / 免疫抑制剤

Research Abstract

Epstein-Barr virus(EBV)-associated post-transplant lymphoproliferative disorder(PTLD) is a serious complication of liver transplantation. We performed periodical molecular EBV monitoring in pediatric living donor liver transplan-tation. Transmission from donor to recipient via graft was highly suspected, because EBV infected cells were identified in 61 grafts of the 82 donors(74.4%). The increase of HLA-DR+CD8+cells were observed in parallel with the decrease in EBV DNA load fol-lowing the reduction of immunosuppressive drugs.

Research Products

• [Journal Article] Effective control of Epstein-Barr virus infection following pediatric liver transplantation by monitoring of viral DNA load and lymphocyte surface markers (2012)
  • Author(s): Imadome K, Fukuda A, Umezawa A
  • Journal Title: Pediatric Transplantation Pages: 100-125
  • Peer Reviewed
• [Journal Article] Effective control of Epstein-Barr virus infection following pediatric liver transplantation by monitoring of viral DNA load and lymphocyte surface markers (2012)
  • Author(s): Imadome K, Fukuda A
  • Journal Title: Pediatric Transplantation Volume: 16(Epub ahead of print) Issue: 7 Pages: 748-757
  • DOI: 10.1111/j.1399-3046.2012.01750.x
  • Peer Reviewed
• [Journal Article] CD4^+T cells have a critical role in the proliferation of EBV-infected T and NK cells (2011)
  • Author(s): Imadome K, Yajima M, Arai A, Nakagawa-Nakazawa A, Kawano F, Ichikawa S, Shimizu N, Yamamoto N, Morio T, Ohga S, Nakamura H, Ito M, Miura O, Komano J, Fujiwara S
  • Journal Title: PLOS Pathogen Volume: (in press) Issue: 10 Pages: 1-14
  • DOI: 10.1371/journal.ppat.1002326
  • Peer Reviewed
• [Journal Article] Successful treatment with rituximab and donor lymphocyte infusions for fulminant EBV-associated lymphoproliferative disease that developed 14 years after unrelated BMT (2011)
  • Author(s): Kawaguchi T, Imadome K
  • Journal Title: Bone Marrow Transplantation Volume: 46(12) Issue: 12 Pages: 1583-1585
  • DOI: 10.1038/bmt.2010.346
  • Peer Reviewed
• [Journal Article] Successful treatment with rituximab and donor lymphocyte infusions for fulminant EBV associated lymphoproliferative disease that developed 14 years after unrelated BMT (2011)
  • Author(s): Kawaguchi T, Imadome K
  • Journal Title: Bone Marrow Transplantation Volume: 31 Pages: 1-3
  • Peer Reviewed
• [Journal Article] Clonal origin of Epstein-Barr virus(EBV)-infected T/NK-cell subpopulations in EBV-positive T/NK-cell lymphoproliferative disorders of childhood (2011)
  • Author(s): Ohga S, Imadome K
  • Journal Title: J Clin.Virol. Volume: 51(1) Pages: 31-37
  • Peer Reviewed
• [Journal Article] Epstein-Barr virus infection after pediatric living-related liver transplantation-management and risk factors (2010)
  • Author(s): Shigeta T, Imadome K, Fukuda A, Umezawa A
  • Journal Title: Transplant Proc Volume: Vol.42, No.10 Issue: 10 Pages: 4178-4780
  • DOI: 10.1016/j.transproceed.2010.09.134
  • Peer Reviewed
• [Journal Article] Epstein-barr virus infection after pediatric living-related liver transplantation-management and risk factors. (2010)
  • Author(s): Shigeta T, Imadome K, Fukuda A
  • Journal Title: Transplant Proc. Volume: 42(10) Pages: 4178-4780
  • Peer Reviewed
• [Journal Article] Autoimmune hemolytic anemia accompanied by reactivation of an Epstein-Barr virus infection with suppressed CTL response to EBV infected cells (2010)
  • Author(s): Arai A, Imadome K
  • Journal Title: Internal Medicine Volume: 49(4) Pages: 325-329
  • Peer Reviewed
• [Presentation] 免疫抑制剤減量にともなうEBV特異的CTLの誘導 (2012)
  • Author(s): 福田晃也,今留謙一,梅澤明弘
  • Organizer: 第21回EBV感染症研究会
  • Place of Presentation: 東京
  • Year and Date: 2012-03-17
• [Presentation] Pre-vention of Post-Transplant Lymphoproliferative Disorder by Monitor-ing of Epstein-Barr Virus Load and Lym-phocyte Phenotypes (2011)
  • Author(s): Fukuda A, Imadome K, Umezawa A
  • Organizer: The 62^<nd> Annual Meeting of the American Association for the study of liver diseases
  • Place of Presentation: San Francisco, U. S. A.
  • Year and Date: 2011-11-05
• [Presentation] Prevention of Post-Transplant Lymphoproliferative Disorder by Monitoring of Epstein-Barr Virus Load and Lymphocyte Phenotypes (2011)
  • Author(s): Fukuda A, Imadome K
  • Organizer: The 62^<nd> Annual Meeting of the American Association for the study of liver diseases
  • Place of Presentation: San Francisco, U.S.A.
  • Year and Date: 2011-11-05
• [Presentation] Epstein-Barr virus infection after pediatric living-related liver transplan-tation : Management and risk factor (2010)
  • Author(s): Shigeta T, Imadome K, Fukuda A, Umezawa A
  • Organizer: XXIII Interational Congress of the Transplanta-tion Society
  • Place of Presentation: Vancouver, Can-ada
  • Year and Date: 2010-08-15
• [Presentation] Epstein-barr virus infection after pediatric living-related liver transplantation : Management and risk factor (2010)
  • Author(s): Shigeta T, Imadome K, Fukuda A
  • Organizer: XXIII Interational Congress of the Transplantation Society
  • Place of Presentation: Vancouver
  • Year and Date: 2010-08-15
• [Presentation] Epstein-Barr virus infection after pediatric living-related liver transplan-tation : Management and risk factor. The International Colleges of Surgeons (2010)
  • Author(s): Shigeta T, Imadome K, Fukuda A, Umezawa A
  • Organizer: The 56th Annual Congress of the Japan Section
  • Place of Presentation: Tokyo
  • Year and Date: 2010-06-12
• [Presentation] Epstein-barr virus infection after pediatric living-related liver transplantation : Management and risk factor (2010)
  • Author(s): Shigeta T, Imadome K, Fukuda A
  • Organizer: The International Colleges of Surgeons, The 56th Annual Congress of the Japan Section
  • Place of Presentation: Tokyo
  • Year and Date: 2010-06-12
• [Presentation] 小児生体肝移植後のEBV感染に関連した合併症例の報告 (2010)
  • Author(s): 福田晃也,今留謙一,梅澤明弘
  • Organizer: 第10回京都肝移植周術期研究会
  • Place of Presentation: 京都市
  • Year and Date: 2010-03-27
• [Presentation] 小児生体肝移植後のEBV感染に関連した合併症例の報告 (2010)
  • Author(s): 福田晃也
  • Organizer: 第10回 京都肝移植周術期研究会
  • Place of Presentation: 京都市
  • Year and Date: 2010-03-27