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Functional analysis of Polycomb-group gene, Pcgf5 in cardiac morphogenesis

Research Project

Project/Area Number 21591405
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Pediatrics
Research InstitutionNational Cardiovascular Center Research Institute

Principal Investigator

SHIRAI Manabu  独立行政法人国立循環器病研究センター, 分子生物学部, 室長 (70294121)

Co-Investigator(Renkei-kenkyūsha) MORISAKI Takayuki  独立行政法人国立循環器病研究センター, 分子生物学部, 部長 (30174410)
MORISAKI Hiroko  独立行政法人国立循環器病研究センター, 分子生物学部, 室長 (40311451)
HONDA Masahiko  独立行政法人国立循環器病研究センター, 分子生物学部, 室員 (10455545)
Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywordsポリコーム遺伝子 / 形態形成 / 発生医学
Research Abstract

To define the functions of one of the Polycomb group(PcG) protein, Pcgf5 for cardiac morphogenesis and know whether mutations of PcG genes are associated with human congenital heart disease, detail analysis of Pcgf5 expression in mouse embryos was performed, and conditional Pcgf5 knockout mice were generated using Cre-loxP system. In the whole-mount in situ hybridization analysis, strong Pcgf5 expression was observed in the developing heart at early stages, meaning Pcgf5 could be involved in cardiac development. The results of western blotting analysis and immunohistochemical analysis showed that some PcG protein, Bmi1, Ring1b and Rae28/Phc1 distributed in the nuclei of embryonic cardiomyocytes and endocardial cells. Expression levels of these proteins were higher at early stages and decreased at the perinatal period. Physical interaction between Ring1b and Bmi1, Ring1b and Rae28/Phc1 in the developing hearts was confirmed with co-immunoprecipitation analysis(Co-IP) and in situ proximal ligation assay(PLA), indicating that Bmi1, Ring1b and Rae28/Phc1 make a Polycomb complex in the embryonic hearts as shown in other tissues. On the other hand, Pcgf5 interacted with Ring1b but not with Rae28/Phc1. These findings suggest that some kinds of Polyocomb complex work in the developing hearts.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (4 results)

All 2012 2011

All Presentation (4 results)

  • [Presentation] Distribution of Polycomb-group proteins in the developing heart2012

    • Author(s)
      Manabu Shirai, Yoshihiro Takihara, Takayuki Morisaki
    • Organizer
      第34回日本分子生物学会
    • Place of Presentation
      横浜
    • Year and Date
      2012-12-13
    • Related Report
      2011 Final Research Report
  • [Presentation] ポリコーム遺伝子群の心臓形態形成に対する発現・機能解析2012

    • Author(s)
      白井学、瀧原義宏、森崎隆幸
    • Organizer
      第10回心臓血管発生研究会
    • Place of Presentation
      福島
    • Year and Date
      2012-10-07
    • Related Report
      2011 Final Research Report
  • [Presentation] Distribution of Polycomb-group proteins in the developing heart2011

    • Author(s)
      白井学
    • Organizer
      第34回日本分子生物学会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2011-12-13
    • Related Report
      2011 Annual Research Report
  • [Presentation] ポリコーム遺伝子群の心臓形態形成に対する発現・機能解析2011

    • Author(s)
      白井学
    • Organizer
      第10回心臓血管発生研究会
    • Place of Presentation
      磐梯熱海温泉ホテル華の湯
    • Year and Date
      2011-10-07
    • Related Report
      2011 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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