| Project/Area Number |
21591419
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Embryonic/Neonatal medicine
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| Research Institution | Nagoya City University |
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Principal Investigator |
MIZUNO Keisuke 名古屋市立大学, 大学院・医学研究科, 研究員 (00405177)
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| Co-Investigator(Kenkyū-buntansha) |
HIDA Hideki 名古屋市立大学, 大学院・医学研究科, 教授 (00305525)
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| Research Collaborator |
MASUDA Tadashi 名古屋市立大学, 大学院・医学研究科, 助教 (10423641)
MISUMI Sachiyo 名古屋市立大学, 大学院・医学研究科, 助教 (70529148)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 脳性麻痺 / 長期モデル / iPS細胞 / 神経系分化誘導 / 細胞移植 / 前駆細胞 / 脳室周囲白質軟化症 / オリゴデンドロサイト / マウスES細胞 / マウスiPS細胞 / 磁気ビーズ / APC陽性 / neurosphere / Argyolphil III染色法 / NeuN / SSEA-1 / 臨床使用薬剤 / 運動機能評価 / TTC染色 / GFAP / OX-41 / ロタロッド |
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| Research Abstract |
It was revealed by the assessment of motor functions(rotarrod, motor deficit score and cylinder test) that a rat model of periventricular leukomalasia(PVL) is appropriate to the model for a longer time. It became possible to induce oligodendrocytes and its progenitors(OPC) from mouse ES cells/iPS cells in vitro as the donor cells to PVL. In transplantation of iPS cells-derived OPC to PVL model rat, grafted cells can survive well in the white matter, revealing that some substances such as trophic factors will be needed for better survival rate.
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