Pathophysilogy of psoriasis : analysis via SOCS1 KO mice in epidermis and T cell.

• Project/Area Number: 21591433
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Dermatology
• Research Institution: Ehime University
• Principal Investigator: HANAKAWA Yasushi 愛媛大学, 大学院・医学系研究科, 講師 (90284398)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 皮膚病理学 / 乾癬 / SOCS1 / STAT / 表皮特異的 / コンディショナルノックアウトマウス / K5-Cre

Research Abstract

To investigate pathophysiology of chronic skin disease of psoriasis, the functional involvement of SOCS1 was analyzed. SOCS1 is the negative regulator of STATs, and epidermis-specific SOCS1 KO mice were investigated. KO mice showed delayed wound healing because of hyper-inflammation by cross talk between keratinocytes and immune cells.

Research Products

• [Journal Article] Interactions between myofibroblast differentiation and epidermogenesis in constructing human living skin equivalents (2012)
  • Author(s): Yang L, Hashimoto K, Tohyama M, Okazaki H, Dai X, Hanakawa Y, Sayama K, Shirakata Y
  • Journal Title: J Dermatol Sci Volume: 65 Pages: 50-7
  • Peer Reviewed
• [Journal Article] Nuclear translocation of phosphorylated STAT3 regulates VEGF-A-induced lymphatic endothelial cell migration and tube formation (2011)
  • Author(s): Okazaki H, Tokumaru S, Hanakawa Y, Shiraishi K, Shirakata Y, Dai X, Yang L, Tohyama M, Hashimoto K, Sayama K
  • Journal Title: Biochem Biophys Res Commun Volume: 412 Pages: 441-5
  • Peer Reviewed
• [Journal Article] Mite allergen is a danger signal for the skin via activation of inflammasome in keratinocytes (2011)
  • Author(s): Dai X, Sayama K, Tohyama M, Shirakata Y, Hanakawa Y, Tokumaru S, Yang L, Hirakawa S, Hashimoto K
  • Journal Title: J Allergy Clin Immunol Volume: 127 Pages: 806-14
  • Peer Reviewed
• [Journal Article] PPARγmediates innate immunity by regulating theα, 25-dihydroxyvitamin D3 induced hBD-3 and cathelicidin in human keratinocytes (2010)
  • Author(s): Dai X, Sayama K, Tohyama M, Shirakata Y, Hanakawa Y, Tokumaru S, Yang L, Hirakawa S, Hashimoto K
  • Journal Title: J Dermatol Sci Volume: 60 Pages: 179-86
  • Peer Reviewed
• [Journal Article] E2 Polyubiquitin conjugating enzyme Ubc13 in keratinocytes is essential for epidermal integrity (2010)
  • Author(s): Sayama K, Yamamoto M, Shirakata Y, Hanakawa Y, Hirakawa S, Dai X, Tohyama M, Tokumaru S, Shin MS, Sakurai H, Akira S, Hashimoto K
  • Journal Title: J Biol Chem Volume: 285 Pages: 30042-9
  • Peer Reviewed
• [Journal Article] Inflammatory mediator TAK1 regulates hair follicle morphogenesis and anagen induction shown by using keratinocyte specific TAK1-deficient mice (2010)
  • Author(s): Sayama K, Kajiya K, Sugawara K, Sato S, Hirakawa S, Shirakata Y, Hanakawa Y, Dai X, Ishimatsu-Tsuji Y, Metzger D, Chambon P, Akira S, Paus R, Kishimoto J, Hashimoto K
  • Journal Title: PLoS One Volume: 5 Pages: 11275-9
  • Peer Reviewed
• [Journal Article] PPAR γ mediates innate immunity by regulating the 1α,25-dihydroxyvitamin D3 induced hBD-3 and cathelicidin in human keratinocytes. (2010)
  • Author(s): Dai X
  • Journal Title: J Dermatol Sci Volume: 60 Pages: 179-186
• [Journal Article] E2 Polyubiquitin-conjugating enzyme Ubc13 in keratinocytes is essential for epidermal integrity. (2010)
  • Author(s): Sayama K
  • Journal Title: J Biol Chem Volume: 285 Pages: 30042-30049
• [Journal Article] Inflammatory mediator TAK1 regulates hair follicle morphogenesis and anagen induction shown by using keratinocyte-specific TAK1-deficient mice. (2010)
  • Author(s): Sayama K
  • Journal Title: PLoS One. Volume: 5
• [Journal Article] IL-17 and IL-22 mediate IL-20 subfamily cytokine production in cultured keratinocytes via increased IL-22 receptor expression (2009)
  • Author(s): Tohyama M, Hanakawa Y, Shirakata Y, Dai X, Yang L, Hirakawa S, Tokumaru S, Okazaki H, Sayama K, Hashimoto K
  • Journal Title: Eur J Immunol Volume: 39 Pages: 2779-88
  • Peer Reviewed
• [Journal Article] Living skin equivalents constructed using human amnions as a matrix (2009)
  • Author(s): Yang L, Shirakata Y, Tokumaru S, Xiuju D, Tohyama M, Hanakawa Y, Hirakawa S, Sayama K, Hashimoto K
  • Journal Title: J Dermatol Sci Volume: 56 Pages: 188-95
  • NAID: 10027398843
  • Peer Reviewed
• [Journal Article] Nodal lymphangiogenesis and metastasis : Role of tumor-induced lymphatic vessel activation in extramammary Paget's disease (2009)
  • Author(s): Hirakawa S, Detmar M, Kerjaschki D, Nagamatsu S, Matsuo K, Tanemura A, Kamata N, Higashikawa K, Okazaki H, Kameda K, Nishida-Fukuda H, Mori H, Hanakawa Y, Sayama K, Shirakata Y, Tohyama M, Tokumaru S, Katayama I, Hashimoto K
  • Journal Title: Am J Pathol Volume: 175 Pages: 2235-48
  • Peer Reviewed
• [Journal Article] IL-17 and IL-22 mediate IL-20 subfamily cytokine production in cultured keratinocytes via increased IL-22 receptor expression (2009)
  • Author(s): Tohyama M, et al.
  • Journal Title: Eur J Immunol 39 Pages: 779-88
  • Peer Reviewed
• [Journal Article] Living skin equivalents constructed using human amnions as a matrix (2009)
  • Author(s): Yang L, et al.
  • Journal Title: J Dermatol Sci. 56 Pages: 188-95
  • Peer Reviewed
• [Journal Article] Nodallymphangiogenesis and metastasis : Role of tumor-induced lymphatic vessel activation in extramammary Pagets disease (2009)
  • Author(s): Hirakawa S, et al.
  • Journal Title: Am J Pathol 175 Pages: 2235-48
  • Peer Reviewed
• [Presentation] dsRNA activates inflammasome in eidermal keratinocytes resulting in the release of active IL-1 beta and IL-18 (2011)
  • Author(s): Dai X
  • Organizer: 36^<th> JSID
  • Place of Presentation: Kyoto
  • Year and Date: 2011-12-09
• [Presentation] Nuclear translocation of Bcl3 and p50 triggers the expression of IL-22 inducible genes in keratinocytes of psoriasis (2011)
  • Author(s): Tohyama M
  • Organizer: 36^<th> JSID
  • Place of Presentation: Kyoto
  • Year and Date: 2011-12-09
• [Presentation] Delsyed wound healing in keratinocytes-specific conditional knockout mice of SOCS1 (2010)
  • Author(s): Hanakawa Y
  • Organizer: 35^<th> JSID
  • Place of Presentation: Wakayama
  • Year and Date: 2010-12-05
• [Presentation] Delsyed wound healing in keratinocytes-specific conditional knockout mice of SOCS1 (2010)
  • Author(s): Hanakawa Y
  • Organizer: 35^<th> JSID
  • Place of Presentation: Wakayama cultural hall, Wakayama
  • Year and Date: 2010-12-05