Hair bulge specific knock-out mouse of tumor suppressor gene

• Project/Area Number: 21591435
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Dermatology
• Research Institution: Ehime University
• Principal Investigator: MURAKAMI Shinji 愛媛大学, 大学院・医学系研究科, 准教授 (50175626)
• Co-Investigator(Kenkyū-buntansha): 平川 聡史 愛媛大学, 医学部附属病院, 講師 (50419511)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 皮膚病理学 / 皮膚腫瘍学 / PTEN / コンディショナルノックアウト / ケラチン15 / Cre-loxP / 扁平上皮癌 / 癌抑制遺伝子 / 癌幹細胞 / マウス / Cre/loxP / 癌 / 血管新生 / 細胞増殖 / リンパ管 / 転移

Research Abstract

To investigate biological specificity and pathological classification of skin cancer stem cell derived tumor, hair bulge stem cell specific knockout mice were made. The transgenic mice that express Cre recombinase via keratin 15promoter were mated with loxP-flanked PTEN mice and hair bulge stem cell specific PTEN knock-out mice were made. Although we analyzed these mice up to one year, no apparent difference was observed between control and knockout mice in terms of tumor progression.

Research Products

• [Journal Article] Possible involvement of CD14+ CD16+ monocyte lineage cells in the epidermal damage of Stevens-Johnson syndrome and toxic epidermal necrolysis (2012)
  • Author(s): Tohyama M, Watanabe H, Murakami S, Shirakata Y, Sayama K, Iijima M, Hashimoto K.
  • Journal Title: Br J Dermatol Volume: 166(2) Pages: 322-330
  • Peer Reviewed
• [Journal Article] Syndrome of inappropriate secretion of antidiuretic hormone induced by tacrolimus in a patient with systemic lupus erythematosus (2011)
  • Author(s): Suemori K, Hasegawa H, Nanba C, Kohno M, Matsumoto T, Kawamoto Y, Murakami S, Sada E, Hashimoto K, Yasukawa M.
  • Journal Title: Mod Rheumatol Volume: 21(1) Pages: 97-100
  • NAID: 10029361489
  • Peer Reviewed
• [Journal Article] VII型コラーゲンが抗原と思われた粘膜類天疱瘡の1例 (2011)
  • Author(s): 難波千佳、白方裕司, 宮脇さおり, 藤山幹子, 花川靖, 白石研, 岡崎秀規, 小田富美子, 村上信司, 橋本公二, 佐山浩二, 福田俊平, 橋本隆
  • Journal Title: 西日本皮膚科 Volume: 73巻6号 Pages: 581-585
  • NAID: 130004475412
  • Peer Reviewed
• [Journal Article] Syndrome of inappropriate secretion of antidiuretic hormone induced by tacrolimus in a patient with systemic lupus erythematosus. (2011)
  • Author(s): Suemori K, Hasegawa H, Nanba C, Kohno M, Matsumoto T, Kawamoto Y, Murakami S, Sada E, Hashimoto K, Yasukawa M
  • Journal Title: Mod Rheumatol Volume: 21 Pages: 97-100
  • NAID: 10029361489
  • Peer Reviewed
• [Journal Article] Possible association of vascular endothelial growth factor with the development of edema in drug-induced hypersensitivity syndrome (2011)
  • Author(s): Hirakawa S, Okazaki H, Sayama K, Tohyama M, Hashimoto K
  • Journal Title: J Dermatol Volume: 38 Pages: 292-294
  • NAID: 10029330846
  • Peer Reviewed
• [Journal Article] Mite allergen is a danger signal for the skin via activation of inflammasome in keratinocytes (2011)
  • Author(s): Dai X, Sayama K, Tohyama M, Shirakata Y, Hanakawa Y, Tokumaru S, Yang L, Hirakawa S, Hashimoto K
  • Journal Title: J Allergy Clin Immunol Volume: 127
  • Peer Reviewed
• [Journal Article] Regulation of pathological lymphangiogenesis requires factors distinct from those governing physiological lymphangiogenesis (2011)
  • Author(s): Hirakawa S
  • Journal Title: J Dermatol Sci Volume: 61 Pages: 85-93
  • NAID: 10030889917
  • Peer Reviewed
• [Journal Article] Multiple lymphadenopathy as an initial sign of extramammary Paget disease (2011)
  • Author(s): Hirakawa S, Tanemura A, Mori H, Katayama I, Hashimoto K
  • Journal Title: Br J Dermatol Volume: 164 Pages: 200-203
  • Peer Reviewed
• [Journal Article] 尋常性乾癬に尋常性天疱瘡を合併した1例カポジ水痘様発疹症が天疱瘡診断の契機となった1例 (2010)
  • Author(s): 浦部由佳里、藤山幹子, 白方裕司, 村上信司, 橋本公二, 中村舞, 西尾有紀子
  • Journal Title: 日本皮膚科学会雑誌 Volume: 120巻14号 Pages: 3109-3114
  • NAID: 10027733598
  • Peer Reviewed
• [Journal Article] 表皮母斑症候群の1例 (2010)
  • Author(s): 佐藤直樹, 藤山幹子, 村上信司, 青木恵美, 橋本公二, 田内久道, 木村美佳, 羽藤直人, 庄野佳孝
  • Journal Title: 西日本皮膚科 Volume: 72巻4号 Pages: 353-355
  • NAID: 10026636874
  • Peer Reviewed
• [Journal Article] 妊婦に生じたZosteriform Angiolymphoid Hyperplasia with Eosinophiliaの1例 (2010)
  • Author(s): 浦部由佳里, 藤山幹子, 村上信司, 橋本公二, 森戸浩明
  • Journal Title: 西日本皮膚科 Volume: 72巻4号 Pages: 356-358
  • NAID: 10026636878
  • Peer Reviewed
• [Journal Article] PPAR_Y mediates innate immunity by regulating the 1α,25-dihydroxyvitamin D3 induced hBD-3 and cathelicidin in human keratinocytes (2010)
  • Author(s): Dai X, Sayama K, Tohyama M, Shirakata Y, Hanakawa Y, Tokumaru S, Yang L, Hirakawa S, Hashimoto K
  • Journal Title: J Dermatol Sci Volume: 60 Pages: 179-186
  • Peer Reviewed
• [Journal Article] E2 Polyubiquitin-conjugating enzyme Ubc13 in keratinocytes is essential for epidermal integrity (2010)
  • Author(s): Sayama K, Yamamoto M, Shirakata Y, Hanakawa Y, Hirakawa S, Dai X, Tohyama M, Tokumaru S, Shin MS, Sakurai H, Akira S, Hashimoto K
  • Journal Title: J Biol Chem Volume: 285 Pages: 30042-30049
  • Peer Reviewed
• [Journal Article] Inflammatory mediator TAK1 regulates hair follicle morphogenesis and anagen induction shown by using keratinocyte-specific TAK1-deficient mice (2010)
  • Author(s): Sayama K, Kajiya K, Sugawara K, Sato S, Hirakawa S, Shirakata Y, Hanakawa Y, Dai X, Ishimatsu-Tsuji Y, Metzger D, Chambon P, Akira S, Paus R, Kishimoto J, Hashimoto K
  • Journal Title: PLoS One Volume: 5
  • Peer Reviewed
• [Journal Article] Nodal lymphangiogenesis and metastasis (2009)
  • Author(s): Hirakawa S, et al.
  • Journal Title: Am J Pathol 175 Pages: 2235-2248
  • Peer Reviewed
• [Presentation] Lymph node lymphangiogenesis (2009)
  • Author(s): 平川聡史
  • Organizer: 7^<th> World Congress on Melanoma
  • Place of Presentation: オーストリア ウィーン
  • Year and Date: 2009-05-15