Development of new therapeutic strategy for skin cancers targeting CD147/Basigin

• Project/Area Number: 21591438
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Dermatology
• Research Institution: Kagoshima University
• Principal Investigator: KANEKURA Takuro 鹿児島大学, 医歯学総合研究科, 教授 (70177509)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: CD147/Basigin / 皮膚癌 / 治療 / 皮膚腫瘍学 / CD147/basigin / CD147 / Rasigin

Research Abstract

We revealed that CD147/basigin promotes proliferation, invasion, and metastasis of malignant melanoma through regulating tumor cell glycolysis and regulates tumor cell adhesion to matrix through phosphorylating the focal adhesion kinase(FAK), a downstream kinase of integrin that co-localizes with CD147 on the cell surface. These findings demonstrate that CD147/basigin could be a target molecule for new therapeutic strategy for skin cancers.

Research Products

• [Journal Article] DNA demethylation of CD40L in CD4^+ T-cells from women with systemic sclerosis-A possible explanation for female susceptibility (2012)
  • Author(s): Lian X
  • Journal Title: Arthritis Rheum Volume: (in press) Issue: 7 Pages: 2338-2345
  • DOI: 10.1002/art.34376
  • Year and Date: 2012-06-26
  • Peer Reviewed
• [Journal Article] Demethylation of TNFSF7 contributes to CD70 overexpression in CD4^+T cells from patients with systemic sclerosis (2012)
  • Author(s): Jiang H, Xiao R, Lian X, Kanekura T, Luo Y, Yin Y, Zhang G, Ynag Y, Wang Y, Zhao M, Lu Q.
  • Journal Title: Clin Immunol Volume: 143 Pages: 39-44
  • Peer Reviewed
• [Journal Article] CD147-targeting siRNA inhibits cell-matrix adhesion of human malignant melanoma cells by phosphrylating focal adhesion kinase (2012)
  • Author(s): Nishibaba R, Higashi Y, Su J, Furukawa T, Kawai K, Kanekura T.
  • Journal Title: J Dermatol Volume: 39 Pages: 63-7
  • Peer Reviewed
• [Journal Article] Evaluation of a newly-developed immunochromatography strip test for diagnosing dermatophytosis (2012)
  • Author(s): Higashi Y
  • Journal Title: Int J Dermatol Volume: 51 Issue: 4 Pages: 406-409
  • DOI: 10.1111/j.1365-4632.2011.05046.x
  • Peer Reviewed
• [Journal Article] CD147-targeting siRNA inhibits cell-matrix adhesion of human malignant melanoma cells by phosphrylating focal adhesion kinase (2012)
  • Author(s): Nishibaba R
  • Journal Title: J Dermatol Volume: 39 Issue: 1 Pages: 63-67
  • DOI: 10.1111/j.1346-8138.2011.01304.x
  • Peer Reviewed
• [Journal Article] Molecular cloning and characterization of a novel human papillomavirus, HPV 126, isolated from a flat wart-like lesion with intracytoplasmic inclusion bodies and a peculiar distribution of Ki-67 and p53 (2012)
  • Author(s): Egawa N
  • Journal Title: Virology Volume: 422 Issue: 1 Pages: 99-104
  • DOI: 10.1016/j.virol.2011.10.011
  • Peer Reviewed
• [Journal Article] Fabry disease : Biochemical, Pathological and Structural Studies of the α-Galactosidase A with E66Q amino acid substitution (2012)
  • Author(s): Togawa T
  • Journal Title: Mol Genet Metab Volume: 105 Issue: 4 Pages: 615-620
  • DOI: 10.1016/j.ymgme.2012.01.010
  • Peer Reviewed
• [Journal Article] Demethylation of TNFSF7 contributes to CD70 overexpression in CD4+ T cells from patients with systemic sclerosis (2012)
  • Author(s): Jiang H
  • Journal Title: Clin Immunol Volume: 143 Issue: 1 Pages: 39-44
  • DOI: 10.1016/j.clim.2012.01.005
  • Peer Reviewed
• [Journal Article] MK615, a Prunus mume Steb.Et Zucc (Ume) extract, attenuates the growth of A375 melanoma cells by inhibiting the ERK1/2-Id-1 pathway (2012)
  • Author(s): Tada K
  • Journal Title: Phytother Res Volume: (in press) Issue: 6 Pages: 833-838
  • DOI: 10.1002/ptr.3645
  • Peer Reviewed
• [Journal Article] Role for E-cadherin as an inhibitory receptor on epidermalγδT cells (2011)
  • Author(s): Uchida Y, Kawai K, Ibusuki A, Kanekura T.
  • Journal Title: J Immunol Volume: 186 Pages: 6945-54
  • Peer Reviewed
• [Journal Article] Retinoic acid exhibits anti-fibrotic activity via the inhibition of 5-lipoxygenase expression in scleroderma fibroblasts (2011)
  • Author(s): Xiao R, Kanekura T, Yoshida N, Higashi Y, Lu QJ, Fukushige T, Kanzaki T.
  • Journal Title: J Dermatol Volume: 38 Pages: 345-53
  • Peer Reviewed
• [Journal Article] Role For E-cadherin as an inhibitory receptor on epidermal γδ T cells (2011)
  • Author(s): Uchida Y
  • Journal Title: J Immunol Volume: 186 Issue: 12 Pages: 6945-6954
  • DOI: 10.4049/jimmunol.1003853
  • Peer Reviewed
• [Journal Article] Efficient uptake of recombinant α-galactosidase A produced with a gene-manipulated yeast by Fabry mice kidneys (2011)
  • Author(s): Tsukimura, et al
  • Journal Title: Mol.Med. Volume: 18 Issue: 1 Pages: 76-82
  • DOI: 10.2119/molmed.2011.00248
  • Peer Reviewed
• [Journal Article] Increased glucose uptake by seborrheic kemtosis detected by positron emission tomography. (2011)
  • Author(s): Ibusuki A
  • Journal Title: J Dermatol Volume: 38 Pages: 104-105
  • Peer Reviewed
• [Journal Article] ER signaling is activated to protect human HaCaT keratinocytes from ER stress induced by environmental doses of UVB (2010)
  • Author(s): Mera K, Kawahara K, Tada K, Kawai K, Hashiguchi T, Maruyama I, Kanekura T.
  • Journal Title: Biochem Biophys Res Commun Volume: 397 Pages: 350-4
  • Peer Reviewed
• [Journal Article] Depletion of CD147 sensitizes human malignant melanoma cells to hydrogen peroxide-induced oxidative stress (2010)
  • Author(s): Li J, Peng L, Wu L, Kuang Y, Su J, Yi M, Hu X, Li D, Xie H, Kanekura T, Chen X.
  • Journal Title: J Dermatol Sci Volume: 58 Pages: 204-10
  • NAID: 10027401177
  • Peer Reviewed
• [Journal Article] CD147 is highly expressed on peripheral blood neutrophils from patients with psoriasis and induces neutrophil chemotaxis (2010)
  • Author(s): Lu H, Kuang Y, Su J, Chang J, Wu L, Kanekura T, Li D, Chen M, Chen X.
  • Journal Title: J Dermatol Volume: 37 Pages: 1053-6
  • NAID: 10028276024
  • Peer Reviewed
• [Journal Article] CD147/basigin promotes progression of malignantmelanoma and other cancers (2010)
  • Author(s): Kanekura T, Xiang Chen
  • Journal Title: J Dermatol Sci Volume: 57 Pages: 149-154
• [Journal Article] Advanced malignant melanoma responding to Prunus mume Sieb.Et Zucc (Ume)extract : Case report and in vitro study. (2010)
  • Author(s): Matsushita S
  • Journal Title: Exp Ther Med Volume: 1 Pages: 569-574
  • Peer Reviewed
• [Journal Article] CD147 is highly expressed on peripheral blood neutrophils from patients with psoriasis and induces neutrophil chemotaxis. (2010)
  • Author(s): Lu H
  • Journal Title: J Dermatol Volume: 37 Pages: 1053-1056
  • Peer Reviewed
• [Journal Article] Depletion of CD147 sensitizes human malignant melanoma cells to hydrogen peroxide-induced oxidative stress. (2010)
  • Author(s): Li J
  • Journal Title: J Dermatol Sci Volume: 58 Pages: 204-210
  • Peer Reviewed
• [Journal Article] ER signaling is activated to protect human HaCaT keratinocytes from ER stress induced by environmental doses of UVB. (2010)
  • Author(s): Mera K
  • Journal Title: Biochem Biophys Res Commun Volume: 397 Pages: 350-354
  • Peer Reviewed
• [Journal Article] Tissue and plasma globotriaosylsphingosine could be a biomarker for assessing enzyme replacement therapy for Fabry disease. (2010)
  • Author(s): Togawa T
  • Journal Title: Biochem Biophys Res Commun Volume: 399 Pages: 716-720
  • Peer Reviewed
• [Journal Article] CD147/basigin promotes progression of malignant melanoma and other cancers (2010)
  • Author(s): Kanekura T
  • Journal Title: J Dermatol Sci 57 Pages: 149-154
  • NAID: 10027399778
  • Peer Reviewed
• [Journal Article] Cutaneous-type adult T-cell leukemia/lymphoma does not primarily showdeletion of NAV3 gene (2010)
  • Author(s): Kawai K
  • Journal Title: J Invest Dermatol 130 Pages: 316-318
  • Peer Reviewed
• [Journal Article] Cutaneous type adult T-cell leukemia/lymphoma is a characteristic subtype and includes erythema/papule and nodule/tumor subgroups (2010)
  • Author(s): Miyata T
  • Journal Title: Int J Cancer 126 Pages: 1521-1528
  • Peer Reviewed
• [Journal Article] RNA interference targeting the CD147 induces apoptosis of multi-drug resistant cancer cells related to XIAP depletion (2009)
  • Author(s): Kuang Y-H, Chen X, Su J, Wu L-S, Liao L-Q, Li-D, Chen Z-S, Kanekura T.
  • Journal Title: Cancer Lett Volume: 276 Pages: 189-95
  • Peer Reviewed
• [Journal Article] Severe loss of invariant NKT cells exhibiting anti-HTLV-1 activity in patients with HTLV-1-associated disorders (2009)
  • Author(s): Azakami K
  • Journal Title: Blood 114 Pages: 3208-3215
  • Peer Reviewed
• [Journal Article] Development of new enzyme replacement therapy for Fabry disease utilizing a modified α-N-acetylgalactosaminidase (2009)
  • Author(s): Tajima Y
  • Journal Title: Am J Hum Genet 85 Pages: 569-580
  • Peer Reviewed
• [Journal Article] DNA demethylation of CD40L in CD4^+T-cells from women with systemic sclerosis-A possible explanation for female susceptibility
  • Author(s): Lian X, Xiao R, Hu X, Kanekura T, Jiang H, Li Y, Wang Y, Yang Y, Zhao M, Lu Q.
  • Journal Title: Arthritis Rheum Volume: (in press)
  • Peer Reviewed
• [Journal Article] Retinoic acid exhibits anti-fibrotic activity via the inhibition of 5-lipoxygenase expression in scleroderma fibroblasts.
  • Author(s): Xiao R
  • Journal Title: J Dermatol Volume: (in press)
  • Peer Reviewed
• [Journal Article] The role of thymidine phosphorylase in the induction of early growth response protein-1 and thrombospondin-1 by 5-fluorouracil in human cancer carcinoma cells
  • Author(s): Matsushita S
  • Journal Title: Int J Oncol (in press)
  • Peer Reviewed
• [Presentation] 好中球性皮膚疾患と顆粒球吸着除去療法 (2012)
  • Author(s): 金蔵拓郎
  • Organizer: 皮膚疾患懇話会(廣仁会)
  • Place of Presentation: 札幌市(招待講演)
  • Year and Date: 2012-02-24
• [Presentation] 好中球性皮膚疾患と顆粒球吸着除去療法 (2012)
  • Author(s): 金蔵拓郎
  • Organizer: 多摩皮膚科専門医会
  • Place of Presentation: 武蔵野市(招待講演)
  • Year and Date: 2012-02-04
• [Presentation] 膿疱性乾癬と好中球性皮膚疾患の治療 (2012)
  • Author(s): 金蔵拓郎
  • Organizer: 第5回熊本乾癬病診連携フォーラム
  • Place of Presentation: 熊本市(招待講演)
  • Year and Date: 2012-01-19
• [Presentation] 皮膚と紫外線 (2012)
  • Author(s): 金蔵拓郎
  • Organizer: 平成24年鹿児島県薬剤師会新春学術講演会
  • Place of Presentation: 鹿児島市(招待講演)
  • Year and Date: 2012-01-15
• [Presentation] 好中球性疾患に対する顆粒球吸着療法 (2011)
  • Author(s): 金蔵拓郎
  • Organizer: 第138回東海皮膚研修セミナー
  • Place of Presentation: 伊勢原市(招待講演)
  • Year and Date: 2011-11-11
• [Presentation] Treatment of pustular psoriasis and neutrophilic dermatoses (2011)
  • Author(s): Kanekura T.
  • Organizer: The 15th Annual Meeting of the Korean Society for psoriasis
  • Place of Presentation: Seoul
  • Year and Date: 2011-09-24
• [Presentation] Treatment of pustular psoriasis and neutrophilic dermatoses (2011)
  • Author(s): Kanekura T
  • Organizer: The 15th Annual Meeting of the Korean Society for psoriasis
  • Place of Presentation: Seoul(招待講演)
  • Year and Date: 2011-09-24
• [Presentation] 膿疱性乾癬に対する顆粒球吸着除去療法 (2011)
  • Author(s): 金蔵拓郎
  • Organizer: 稀少難治性皮膚疾患に関する調査研究班平成23年度第1回総会
  • Place of Presentation: 東京
  • Year and Date: 2011-07-08
• [Presentation] CD147/basiginと癌日本皮膚科学会東北六県合同地方会学術大会 (2011)
  • Author(s): 金蔵拓郎
  • Organizer: 第353回例会
  • Place of Presentation: 仙台市
  • Year and Date: 2011-02-05
• [Presentation] CD147/basiginと癌 (2011)
  • Author(s): 金蔵拓郎
  • Organizer: 日本皮膚科学会東北六県合同地方会学術大会第353回例会
  • Place of Presentation: 仙台市
  • Year and Date: 2011-02-05
• [Presentation] The treatment of the neutrophilic dermatoses. Symposium 44 : Neutrophilic dermatoses (2011)
  • Author(s): Kanekura T.
  • Organizer: The 22nd World Congress of Dermatology
  • Place of Presentation: Seoul
• [Presentation] The treatment of the neutrophilic dermatoses (2011)
  • Author(s): Kanekura T
  • Organizer: 22nd World Congress of Dermatology
  • Place of Presentation: Seoul(招待講演)
• [Presentation] Analysis of illioinguinal lymph node dissection in patients with melanoma (2011)
  • Author(s): Matsushita S
  • Organizer: The 41st Annual Meeting of European Society for Dermatological Research
  • Place of Presentation: Barcelona
• [Presentation] Effective sterilization of methicillin-resistant staphylococcus aureus (MRSA) with an ultrasonic levitation washer disinfector and silver electrolysis and ozone oxidation (2011)
  • Author(s): Tamai M
  • Organizer: The 41st Annual Meeting of European Society for Dermatological Research
  • Place of Presentation: Barcelona
• [Presentation] Do γδ T cells play a role in alopecia areata pathogenesis? (2011)
  • Author(s): Arakawa A
  • Organizer: The 36^<th> Annual Meeting of the Japanese Society for Investigative Dermatology
  • Place of Presentation: Kyoto
• [Presentation] The skin-specific NKG2D ligand H60c directly triggers cytotoxicity in qpidermal γδ T cells (2011)
  • Author(s): Ibusuki A
  • Organizer: The 36^<th> Annual Meeting of the Japanese Society for Investigative Dermatology
  • Place of Presentation: Kyoto
• [Presentation] Specific expression of water channels, aquaporin-1 and-3, in burned skin (2011)
  • Author(s): Kubo H
  • Organizer: The 36^<th> Annual Meeting of the Japanese Society for Investigative Dermatology
  • Place of Presentation: Kyoto
• [Presentation] 強皮症の病態と治療 (2010)
  • Author(s): 金蔵拓郎
  • Organizer: 第88回日本皮膚科学会大分地方会
  • Place of Presentation: 大分市
  • Year and Date: 2010-11-13
• [Presentation] 膿疱性乾癬に対する顆粒球吸着除去療法-臨床効果と奏効機序の検討- (2010)
  • Author(s): 金蔵拓郎
  • Organizer: 第25日本乾癬学会Keynote Lecture
  • Place of Presentation: 宇部市
  • Year and Date: 2010-09-04
• [Presentation] 強皮症の病態と治療 (2010)
  • Author(s): 金蔵拓郎
  • Organizer: 第34回鹿児島膠原病研究会
  • Place of Presentation: 鹿児島市
  • Year and Date: 2010-07-15
• [Presentation] 好中球性皮膚疾患と顆粒球吸着除去療法 (2010)
  • Author(s): 金蔵拓郎
  • Organizer: 日本皮膚科学会山形地方会総会第350回例会
  • Place of Presentation: 山形市
  • Year and Date: 2010-04-11
• [Presentation] E-cadherin on epidermalγδT cells is an inhibitory receptor (2010)
  • Author(s): Uchida Y, Kawai K, Kanekura T.
  • Organizer: The 40th Annual Meeting of European Society for Dermatological Reseach
  • Place of Presentation: Helsinki, Finland
• [Presentation] ER signaling is activated to protect human keratinocytes from ER stress provoked by the environmental dose UVB (2010)
  • Author(s): Mera K, Kawahara K, Tada K, Kawai K, Hashiguchi T, Maruyama I, Kanekura T.
  • Organizer: The 40th Annual Meeting of European Society for Dermatological Reseach
  • Place of Presentation: Helsinki, Finland
• [Presentation] A CD-147-targeting siRNA enhances the cell adhesion of human malignant cells by phosphorylating focal adhesion kinase (2010)
  • Author(s): Kamiwada R, Higashi Y, Kawai K, Kanekura T.
  • Organizer: The 70th Annual Meeting of the Society for Investigative Dermatology
  • Place of Presentation: Atlanta, USA
• [Presentation] MK615, a Japanese apricot extract containing triterpenoids, induces apoptosis in malignant melanoma cells and a patient with malignant melanoma. (2010)
  • Author(s): Tada K
  • Organizer: The 70th Annual Meeting of the Society for Investigative Dermatology.
  • Place of Presentation: Atlanta, USA
• [Presentation] A CD-147-targeting siRNA enhances the cell adhesion of human malignant cells by phosphorylating focal adhesion kinase. (2010)
  • Author(s): Kamiwada R
  • Organizer: The 70th Annual Meeting of the Society for Investigative Dermatology.
  • Place of Presentation: Atlanta, USA
• [Presentation] The role of thymidine phosphorylase in the induction of early growth response protein-1 and thrombospondin-1 by 5-fluorouracil in human cancer carcinoma cells. (2010)
  • Author(s): Matsushita S
  • Organizer: The 40th Annual Meeting of European Society for Dermatological Research.
  • Place of Presentation: Helsinki, Finland
• [Presentation] ER signaling is activated to protect human keratinocytes from ER stress provoked by the environmental dose UVB. (2010)
  • Author(s): Mera K
  • Organizer: The 40th Annual Meeting of European Society for Dermatological Research.
  • Place of Presentation: Helsinki, Finland
• [Presentation] E-cadherin on epidermal γδT cells is an inhibitory receptor. (2010)
  • Author(s): Uchida Y
  • Organizer: The 40th Annual Meeting of European Society for Dermatological Research.
  • Place of Presentation: Helsinki, Finland
• [Presentation] Retinoic acids exhibit anti-fibrotic activity via the inhibition of 5-lipoxygenase expression in scleroderma fibroblasts. (2010)
  • Author(s): Xiao R
  • Organizer: The 1st Eastern Asia Dermatology Congress.
  • Place of Presentation: Fukuoka, Japan
• [Presentation] 膠原病の皮膚症状 ―臨床と病理― (2009)
  • Author(s): 金蔵拓郎
  • Organizer: 環中海皮膚井病理組織研究会
  • Place of Presentation: 米子市
  • Year and Date: 2009-11-26
• [Presentation] CD147/Basiginと皮膚癌 (2009)
  • Author(s): 金蔵拓郎
  • Organizer: 第54回日本皮膚科学会高知地方会
  • Place of Presentation: 高知市
  • Year and Date: 2009-09-19
• [Presentation] CD147/Basigin と皮膚癌 (2009)
  • Author(s): 金蔵拓郎
  • Organizer: 第54回日本皮膚科学会高知地方会
  • Place of Presentation: 高知市
  • Year and Date: 2009-09-19
• [Presentation] 好中球と皮膚疾患 ―病態と治療― (2009)
  • Author(s): 金蔵拓郎
  • Organizer: 第8回香川皮膚免疫研究会
  • Place of Presentation: 高松市
  • Year and Date: 2009-09-18
• [Presentation] 好中球と皮膚疾患 (2009)
  • Author(s): 金蔵拓郎
  • Organizer: 平成21年度日本皮膚科学会西部支部主催生涯教育セミナー
  • Place of Presentation: 福岡市
  • Year and Date: 2009-06-27
• [Presentation] 好中球と皮膚疾患 -治療への新たなアプローチ- (2009)
  • Author(s): 金蔵拓郎
  • Organizer: 東海皮膚科治療勉強会
  • Place of Presentation: 名古屋市
  • Year and Date: 2009-05-23
• [Presentation] A small interfering p53R2-targeting RNA inhibited the proliferation and enhanced the chemosensitivity of malignant melanoma (2009)
  • Author(s): Matsushita S
  • Organizer: The 7th World fo Dermato-Oncology
  • Place of Presentation: Vienna, Austria