New melanoma model using transgenic medaka fish

• Project/Area Number: 21591444
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Dermatology
• Research Institution: Keio University
• Principal Investigator: MATSUZAKI Yuriko 慶應義塾大学, 医学部, 助教 (40255435)
• Co-Investigator(Renkei-kenkyūsha): SAYA Hideyuki 慶應義塾大学, 医学部, 教授 (80264282)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: メダカ / 遺伝子導入 / 癌モデル生物

Research Abstract

The purpose of this study is to construct the stable tumor bearing medaka line and to apply the line to drug screening. We intended to establish the melanoma model which can be detected tumors without autopsy for chemical screening in water tank. With cross transgenic(Tg)-tyr : HRAS^<G12V> medaka with Tg-hsp : cre medaka, we could obtain medaka line induced human HRAS^<G12V> by heat treatment. All of the double Tg medaka had melanophore hyperplasia or melanoma at six month post fertilization. Preliminary administration of sorafenib to this double Tg medaka having tumor showed significant inhibition of tumor growth and elongation of longevity.

Research Products

• [Journal Article] Induction of ZEB by inactivation of RB is a key determinant of the mesenchymal phenotype of breast cancer (2012)
  • Author(s): Arima Y, Hayashi H, Sasaki M, Hosonaga M, Goto TM, Chiyoda T, Kuninaka S, Shibata T, Ohata H, Nakagama H, Taya Y and Saya H
  • Journal Title: J Biol Chem Volume: 287 Pages: 7896-7906
  • Peer Reviewed
• [Journal Article] Modulation of glucose metabolism by CD44 contributes to antioxidant status and drug resistance in cancer cells (2012)
  • Author(s): Tamada M, Nagano O, Tateyama S, Ohmura M, Yae T, Ishimoto T, Sugihara E, Onishi N, Yamamoto T, Yanagawa H, Suematsu M and Saya H
  • Journal Title: Cancer Res Volume: 72 Pages: 1438-1448
  • Peer Reviewed
• [Journal Article] Loss of p16 expression is associated with the stem cell characteristics of surface markers and therapeutic resistance in estrogen receptor-negative breast cancer (2012)
  • Author(s): Arima Y, Hayashi N, Hayashi H, Sasaki M, Kai K, Sugihara E, Abe E, Yoshida A, Mikami S, Nakamura S and Saya H
  • Journal Title: Int J Cancer Volume: 130 Pages: 2568-2579
  • Peer Reviewed
• [Journal Article] Invasion precedes tumor mass formation in a malignant brain tumor model of genetically modified neural stem cells (2011)
  • Author(s): Sampetrean O, Saga I, Nakanishi M, Sugihara E, Fukaya R, Onishi N, Osuka A, Akahata M, Kai K, Sugimoto H, Hirao A, and Saya H
  • Journal Title: Neoplasia Volume: 13 Pages: 784-791
  • Peer Reviewed
• [Presentation] シーズ探索その1「大学における基礎研究を創薬に結び付けるために」 (2012)
  • Author(s): 佐谷秀行
  • Organizer: 第12回抗悪性腫瘍開発フォーラム
  • Place of Presentation: 東京都、吉田富三記念講堂、がん研究会がん研究所
  • Year and Date: 2012-02-18
• [Presentation] HRAS^<G12V> transgenic medaka fish develop melanoma with high frequency (2011)
  • Author(s): 松崎ゆり子, 佐谷秀行, 他
  • Organizer: The 1^<st> strategic meeting for medaka research
  • Place of Presentation: 愛知県: Okazaki conference center
  • Year and Date: 2011-11-23
• [Presentation] HRAS^<G12V> transgenic medaka fish develop melanoma with high frequency (2011)
  • Author(s): 松崎ゆり子
  • Organizer: The 1^<st> strategic meeting for medaka research
  • Place of Presentation: 愛知県:Okazaki conference center
  • Year and Date: 2011-11-23
• [Presentation] 癌幹細胞の治療抵抗性を制御する分子機構。パネルディスカッション3「がん幹細胞研究の進展と治療展開」 (2011)
  • Author(s): 佐谷秀行
  • Organizer: 第49回日本癌治療学会学術集会
  • Place of Presentation: 愛知県、名古屋国際会議場
  • Year and Date: 2011-10-27
• [Presentation] HRAS^<G12V> transgenic medaka fish develop melanoma with high frequency (2011)
  • Author(s): 松崎ゆり子, 佐谷秀行
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 愛知県:名古屋国際会議場
  • Year and Date: 2011-10-04
• [Presentation] HRAS^<G12V> transgenic medaka fish develop melanoma with high frequency (2011)
  • Author(s): 松崎ゆり子
  • Organizer: 日本癌学会学術総会
  • Place of Presentation: 愛知県:名古屋国際会議場
  • Year and Date: 2011-10-04
• [Presentation] がん幹細胞の治療抵抗性メカニズム (2011)
  • Author(s): 佐谷秀行
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 愛知県:名古屋国際会議場
  • Year and Date: 2011-10-03
• [Presentation] がん幹細胞の性質を制御する分子機構とその治療戦略 (2011)
  • Author(s): 佐谷秀行
  • Organizer: CBI学会第316回研究講演会
  • Place of Presentation: 東京都、総評会館
  • Year and Date: 2011-04-18
• [Presentation] New melanoma model using transgenic medaka fish (2010)
  • Author(s): 松崎ゆり子, 佐谷秀行, 他
  • Organizer: 日本分子生物学会年会
  • Place of Presentation: 兵庫県:神戸国際会議場
  • Year and Date: 2010-12-09
• [Presentation] メダカ悪性黒色腫モデル系の構築 (2010)
  • Author(s): 松崎ゆり子, 佐谷秀行, 他
  • Organizer: 第16回小型魚類研究会
  • Place of Presentation: 埼玉県:プラザイースト
  • Year and Date: 2010-09-19
• [Presentation] New melanoma model using transgenic medaka fish (2010)
  • Author(s): 松崎ゆり子
  • Organizer: 日本分子生物学会
  • Place of Presentation: 神戸
• [Presentation] 遺伝子改変メダカを用いた発癌モデルの構築 (2009)
  • Author(s): 松崎ゆり子, 佐谷秀行
  • Organizer: 日本癌学会学術総会
  • Place of Presentation: 神奈川県:横浜国際会議場
  • Year and Date: 2009-10-01
• [Presentation] 遺伝子改変メダカを用いた発癌モデルの構築 (2009)
  • Author(s): 松崎ゆり子
  • Organizer: 日本癌学会
  • Place of Presentation: 横浜
  • Year and Date: 2009-10-01
• [Presentation] メダカを用いた悪性黒色腫モデル系の構築 (2009)
  • Author(s): 松崎ゆり子, 佐谷秀行
  • Organizer: 第15回小型魚類研究会
  • Place of Presentation: 愛知県名古屋大学野依記念学術交流館
  • Year and Date: 2009-09-12
• [Remarks]
  • URL: http://www.genereg.jp/html/research/2011/08/post.html
• [Remarks]
  • URL: http://www.genereg.jp/html/research/2011/08/post.html