Signaling in response to oxidative stress disorder caused by ultraviolet

• Project/Area Number: 21591446
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Dermatology
• Research Institution: Tokai University
• Principal Investigator: OZAWA Akira 東海大学, 医学部, 教授 (20096209)
• Co-Investigator(Kenkyū-buntansha): TAKEKOSHI Susumu 東海大学, 医学部, 准教授 (70216878) ; IKOMA Norihiro 東海大学, 医学部, 講師 (40407979)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 皮膚病理学 / 酸化ストレス / 乾癬 / 増殖・分化異常 / MAPキナーゼ / 幹細胞マーカー / シグナル伝達 / 慢性炎症性皮膚疾患 / ヒト皮膚組織 / 紫外線 / 抗酸化酵素 / レーザーマイクロダイセクション

Research Abstract

Intended for human skin tissue was exposed to sunlight long period of time, we analyzed that oxidative stress injury, the expression of antioxidant enzymes and oxidative stress signaling. As a result, oxidative DNA is a marker of nucleic acid damage by reactive oxygen species showed no change. On the other hand, HNE become a marker of lipid peroxidation was increased. HNE is not co-localized with collagen, but co-localized with elastin. Also, in a variety of antioxidant enzymes, induction of HO-2 expression is non-inductive type is made for the first time became clear. In addition, we were investigated by using the skin lesions of psoriasis skin disease as a model. Cells were co-localized with A and B had failed oxidative stress. The failed cell oxidative stress was correlated with BSA which is a measure of the skin lesions of psoriasis.

Research Products

• [Journal Article] Protein oxidative damage and heme oxygenase in sunlight-exposed human skin : roles of MAPK responses to oxidative stress (2010)
  • Author(s): Akasaka E, Takekoshi S, Horikoshi Y, Toriumi K, Ikoma N, Mabuchi T, Tamiya S, Matsuyama T, Ozawa A
  • Journal Title: Tokai J Exp Clin Med Volume: Vol.35、No.4 Pages: 152-64
  • Peer Reviewed
• [Journal Article] Protein oxidative damage and heme oxygenase in sunlight-exposed human skin : Roles of MAPK responses to oxidative stress (2010)
  • Author(s): Emiko Akasaka, et al
  • Journal Title: Tokai journal of experimental and clinical medicine Volume: 4 Pages: 152-164
  • Peer Reviewed
• [Presentation] Oxidative stress mediated NF-kB activation regulates epidermal cell proliferation and differentiation in psoriasis (2011)
  • Author(s): Tomoko Kojima, Emiko Akasaka, Masayuki Kato, Tomotaka Mabuchi, Norihiro Ikoma, Takashi Matsuyama, Akira Ozawa, Yosuke Horikoshi, Kentarou Toriumi, Hideyo Henzan, Susumu Takekoshi, Naoya Nakamura
  • Organizer: 36^<th> The Japanase Society for Investigative Dermatology
  • Place of Presentation: 京都国際会議場(京都市)
• [Presentation] Oxidative stress mediated NF-kB activation regulates epidermal cell proliferation and differentiation in psoriasis (2011)
  • Author(s): 小島知子, 赤坂江美子, 生駒憲広, 竹腰進, 小澤明
  • Organizer: 日本研究皮膚科学会第36回年次学術大会・総会
  • Place of Presentation: 国立京都国際会館
• [Presentation] Relationship between antioxidative enzymes and oxidative stress using immunohistochemical analysis in chronic UV-exposed human skin (2010)
  • Author(s): Emiko Akasaka, Susumu Takekoshi, Shoutarou Tsutiya, Kentarou Toriumi, Tomotaka Mabuchi, Norihiro Ikoma, Shiho Tamiya, Takashi Matsuyama, Muneo Miyasaka, Yoshiyuki Osamura and Akira Ozawa
  • Organizer: 19th Congress of the EADV
  • Place of Presentation: Swedish Exhibition Centre(ヨーテボリ)
• [Presentation] Relationship between antioxidative enzymes and oxidative stress using immunohistochemical analysis in chronic UV-exposed human skin (2010)
  • Author(s): Emiko Akasaka
  • Organizer: 19th Congress of the EADV
  • Place of Presentation: ヨーテボリ,スウェーデン
• [Presentation] Antioxidantive enzymes and increased oxidative stress in chronic UV exposed himan skin (2009)
  • Author(s): 赤坂江美子
  • Organizer: 日本研究皮膚科学会
  • Place of Presentation: JALリゾートシーホークホテル福岡
  • Year and Date: 2009-12-01
• [Presentation] 皮膚組織における紫外線・酸化ストレス障害に応答するシグナル伝達と抗酸化システムの検討 (2009)
  • Author(s): 赤坂江美子、竹腰進、鳥海健太郎、土屋翔太郎、宮坂宗男、長村義之、小澤明
  • Organizer: 第98回日本病理学会総会
  • Place of Presentation: 国立京都国際会館(京都市)
  • Year and Date: 2009-05-03
• [Presentation] 皮膚組織における紫外線酸化ストレス障害に応答するシグナル伝達と抗酸化システムの検討 (2009)
  • Author(s): 赤坂江美子
  • Organizer: 日本病理学会
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 2009-05-01
• [Presentation] The relationship between antioxidative enzymes and oxidative stress using immunohistochemical analysis in chronic UV exposed human skin clinically (2009)
  • Author(s): Emiko Akasaka, Susumu Takekoshi, Shoutarou Tsutiya, Kentarou Toriumi, Mabuchi Tomotaka, Norihiro Ikoma, Shiho Tamiya, Takashi Matusyama, Muneo Miyasaka, Yoshiyuki Osamura, Akira Ozawa
  • Organizer: 34^<th> The Japanase Society for Investigative Dermatology
  • Place of Presentation: JALリゾートシーホークホテル福岡(福岡市)