Identification of melanoma-specific serum biomarker and its clinical application

• Project/Area Number: 21591451
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Dermatology
• Research Institution: Shizuoka Cancer Center Research Institute
• Principal Investigator: AKIYAMA Yasuto 静岡県立静岡がんセンター(研究所), 免疫治療研究部, 部長 (70222552)
• Co-Investigator(Renkei-kenkyūsha): KIYOHARA Yoshio 静岡がんセンター病院, 皮膚科, 部長 (70205037)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: メラノーマ / バイオマーカー / プロテオーム / 免疫学

Research Abstract

We identified pro-platlet basic protein precursor(PPBP) as novel melanoma-biomarker previously. In the present study, we are aiming at developing specific anti-PPBP antibody and establish PPBP-specific ELISA system contributing to a clinical application. The conventional method to make hybridoma using spleen cells was not successful ; however novel approach using regional lymph node enabled us to obtain many functional hybridoma clones. We plan to construct a high sensitive ELISA specific for PPBP through a screening of newly obtained hybridomas.

Research Products

• [Journal Article] Identification of cytomegalovirus(CMV) pp65 antigenspecific human monoclonal antibodies using single B cell-based antibody gene cloning from melanoma patients (2011)
  • Author(s): Iizuka A, Komiyama M, Tai S, Oshita C, Kurusu A, Kume A, Ozawa K, Nakamura Y, Ashizawa T, Yamamoto A, Yamazaki N, Yoshikawa S, Kiyohara Y, Yamaguchi K, Akiyama Y
  • Journal Title: Immunol Lett Volume: 135 Pages: 64-73
  • Peer Reviewed
• [Journal Article] Antitumor activity of a novel small molecule STAT3 inhibitor (2011)
  • Author(s): Ashizawa T, Miyata H, Ishii H, Oshita C, Matsuno K, Masuda Y, Furuya T, Okawara T, Otsuka M, Ogo N, Asai A, and Akiyama Y
  • Journal Title: Int J Oncol Volume: 38(5) Pages: 1245-52
  • Peer Reviewed
• [Journal Article] Analysis of HLA-A24-restricted peptides of Carcinogenic embryonic antigen(CEA) using a novel structure-based peptide-HLA docking algorithm (2011)
  • Author(s): Nakamura Y, Oshita C, Iizuka A, Ashizawa T, Saito S, Yamaguchi S, Kondo H, Yamaguchi K, Akiyama Y
  • Journal Title: Cancer Sci Volume: 102(4) Pages: 690-6
  • Peer Reviewed
• [Journal Article] Analysis of HLA-A24-restricted peptides of carcinoembryonic antigen using a novel structure-based peptide-HLA docking algorithm (2011)
  • Author(s): Yoji Nakamura
  • Journal Title: Cancer Sci Volume: 102(4) Issue: 4 Pages: 690-696
  • DOI: 10.1111/j.1349-7006.2011.01866.x
  • Peer Reviewed
• [Journal Article] Identification of melanoma antigens using SERPA(serological proteome approach) (2010)
  • Author(s): Suzuki A, Iizuka A, Komiyama M, Takikawa M, Kume A, Tai S, Ohshita C, Kurusu A, Nakamura Y, Yamamoto A, Yamazaki N, Yoshikawa S, Kiyohara Y, Akiyama Y
  • Journal Title: Cancer Genomics and Proteomics Volume: 7(1) Pages: 17-23
  • Peer Reviewed
• [Journal Article] TCR-βRepertoire Analysis of Antigen-specific Single T Cells Using a High-density Microcavity Array (2010)
  • Author(s): Arakaki A, Ooya K, Akiyama Y, Hosokawa M, Komiyama M, Tai S, Iizuka A, Yamaguchi K, Matsunaga T
  • Journal Title: Biotechnol Bioeng Volume: 106(2) Pages: 311-318
  • Peer Reviewed
• [Journal Article] CIG-DB : the database for human or mouse immunoglobulin and T cell receptor genes available for cancer studies (2010)
  • Author(s): Nakamura Y, Komiyama T, Furue M, Gojobori T, Akiyama Y
  • Journal Title: BMC Bioinformatics Volume: 11 Pages: 398-398
  • Peer Reviewed
• [Journal Article] S-trityl-L-cysteine derivative indices caspase-independent cell death in K562 human chronic myeloid leukemia cell line (2010)
  • Author(s): Shimizu M, Ishii H, Ogo N, Unno Y, Matsuno K, Sawada J, Akiyama Y, Asai A
  • Journal Title: Cancer Lett Volume: 298(1) Pages: 99-106
  • Peer Reviewed
• [Journal Article] Identification of a new series of STAT3 inhibitors by virtual screening (2010)
  • Author(s): Matsuno K, Masuda Y, Uehara Y, Sato H, Muroya A, Takahashi O, Yokotagawa T, Furuya T, Okawara T, Otsuka M, Ogo N, Ashizawa T, Oshita C, Tai S, Ishii H, Akiyama Y, Asai A
  • Journal Title: ACS Med Chem Lett Volume: 1(8) Pages: 371-375
  • Peer Reviewed
• [Journal Article] Identification of melanoma-specific serological markers using proteomic analyses (2009)
  • Author(s): Takikawa M, Akiyama Y, Ashizawa T, Yamamoto A, Yamazaki N, Kiyohara Y, Oku N, Yamaguchi K
  • Journal Title: Proteomics-CA Pages: 552-562
  • Peer Reviewed
• [Journal Article] Characterization of cytomegalovirus(CMV) pp65-HLA-A24 peptide-specific CTL lines from metastatic melanoma patients (2009)
  • Author(s): Akiyama Y, Tai S, Komiyama M, Takikawa M, Ohshita C, Yamamoto A, Yamazaki N, Kiyohara Y
  • Journal Title: Oncol Rep Volume: 22(1) Pages: 185-191
  • Peer Reviewed
• [Journal Article] 一細胞分離技術を用いたがん特異的免疫細胞の遺伝子スクリーニング法の開発 (2009)
  • Author(s): 秋山靖人
  • Journal Title: 応用物理 Volume: 78(12) Pages: 1123-1127
  • NAID: 10026198589
  • Peer Reviewed
• [Journal Article] Disrupting actin filaments promotes efficient transfection of a leukemic cell line using cell adhesive protein-embedded carbonate apatite particles (2009)
  • Author(s): Kutsuzawa K, Tada S, Hossain S, Fukuda K, Maruyama K, Akiyama Y, Akaike T, Chowdhury EH
  • Journal Title: Anal Biochem Volume: 388 Pages: 164-166
  • Peer Reviewed
• [Presentation] HLA-ペプチドドッキング解析に基づいた免疫創薬的アプローチ (2011)
  • Author(s): 秋山靖人
  • Organizer: 第20回日本組織適合性学会大会
  • Place of Presentation: ツインメッセ静岡静岡市
  • Year and Date: 2011-08-31
• [Presentation] HLA-ペプチドドッキング解析に基づいた免疫創薬的アポローチ (2011)
  • Author(s): 秋山靖人
  • Organizer: 第20回日本組織適合性学会
  • Place of Presentation: 静岡市
  • Year and Date: 2011-08-31
• [Presentation] Novel approach to the characterization of melanoma-peptide-specific CTL lines from metastatic melanoma patients (2011)
  • Author(s): Yasuto Akiyama
  • Organizer: China-Japan symposium on Cancer Research
  • Place of Presentation: Shenzhen, China
  • Year and Date: 2011-05-19
• [Presentation] Novel approach to the characterization of melanoma associatedpeptide-specific CTL lines from metastatic melanoma patients (2011)
  • Author(s): Akiyama Y
  • Organizer: China-Japan Symposium on Cancer Reasearch
  • Place of Presentation: Pattaya Hotel, Shenzhen, China
• [Book] Novel approach to the characterization of melanoma-peptide-specific CTL lines from metastatic melanoma patients (2011)
  • Author(s): Akiyama Y, et al
  • Total Pages: 495
  • Publisher: Intech
• [Book] Chaper23 : Novel approach to the characterization of melanoma-peptide-specific CTL lines from metastatic melanoma patients. Breakthroughs in melanoma research, ISBN 978-953-307-291-3 (2011)
  • Author(s): Yasuto Akiyama
  • Publisher: INTECH
• [Book] シングルセル解析の最前線第4章mRNAをターゲットとしたデジタル精密計測技術の開発10.1細胞分離技術を用いたがん特異的免疫細胞の遺伝子スクリーニング法の開発 (2010)
  • Author(s): 秋山靖人、新垣篤史
  • Total Pages: 274
  • Publisher: シーエムシー出版
• [Patent(Industrial Property Rights)] プロテオミクス解析を用いたメラノーママーカーの同定 (2008)
  • Inventor(s): 秋山靖人、瀧川雅子
  • Industrial Property Rights Holder: 秋山靖人、瀧川雅子
  • Industrial Property Number: 2008-149728
  • Filing Date: 2008-06-06