Development of the therapy for psoriasis by using mouse model

• Project/Area Number: 21591476
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Dermatology
• Research Institution: Kochi University
• Principal Investigator: TARUTANI Masahito 高知大学, 教育研究部・医療学系, 准教授 (30301261)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: Raf / IL-17乾癬 / モデルマウス / Stat3 / IL-17 / 乾癬

Research Abstract

Psoriasis-like skin hyperkeratosis appeared by expressing Raf protein in mice skin by using tamoxifen induced Raf transgenic mice. And the cytokines, which increase in psoriatic skin also elevated in these mice skin. As neutrophil infiltration was seen in these Raf induced skin hyperkeratosis, we treated these mice with anti-neutrophil antibody. The result is that hyperkeratosis was diminished. Next, we disrupted Stat3 in these Raf induced mouse skin using stat3 knockout mice, then Raf-induced skin hyperkeratosis was diminished. These data suggest that Stat3 plays an important role for the pathogenesis of Raf induced psoriasis-like mouse skin hyperkeratosis

Research Products

• [Journal Article] Rafの活性化により引き起こされる乾癬様皮疹はStat3シグナルを必要とする (2012)
  • Author(s): 樽谷勝仁
  • Journal Title: 第26回角化症研究会記録集 Pages: 58-61
• [Journal Article] GPHR-dependent functions of the Golgi apparatus are essential for formation of lamellar granules and the skin barrier (2012)
  • Author(s): Tarutani M
  • Journal Title: J Invest Dermatol Volume: (in press)
  • Peer Reviewed
• [Journal Article] Miyoshi K, Takaishi M, Nakajima K, Ikeda M, Kanda T, Tarutani M, Iiyama T, Asao N, DiGiovanni J, Sano S. Stat3 as a therapeutic target for the treatment of psoriasis : a clinical feasibility study with STA-21, a Stat3 inhibitor (2011)
  • Author(s): Miyoshi K, Takaishi M, Nakajima K, Ikeda M, Kanda T, Tarutani M, Iiyama T, Asao N, DiGiovanni J, Sano S.
  • Journal Title: J Invest Dermatol Volume: 131(1) Pages: 108-117
  • Peer Reviewed
• [Journal Article] Stat3 as a therapeutic target for the treatment of psoriasis : a clinical feasibility study with STA-21, a Stat3 inhibitor. (2011)
  • Author(s): Miyoshi K, Takaishi M, Nakajima K, Ikeda M, Kanda T, Tarutani M, Iiyama T, Asao N, Digiovanni J, Sano S.
  • Journal Title: J Invest Dermatol Volume: 131(1) Pages: 108-117
  • Peer Reviewed
• [Journal Article] Neutrophil-dominant psoriasis-like skin inflammation induced by epidermal-specific expression of Raf in mice (2010)
  • Author(s): Tarutani M, Imai Y, Yasuda K, Tsutsui H, Nakanishi K, Yamanishi K.
  • Journal Title: J Dermatol S Volume: 58(1) Pages: 28-35
  • NAID: 10027400286
  • Peer Reviewed
• [Journal Article] Neutrophil-dominant psoriasis-like skin inflammation induced by epidermal-specific expression of Raf in mice (2010)
  • Author(s): Masahito Tarutani, Yasutomo Imai, Koubun Yasuda, Hiroko Tsutsui, Kenji Nakanishi, Kiyofumi Yamanishi
  • Journal Title: Journal of Dermatological Science Volume: 58(1) Pages: 28-35
  • NAID: 10027400286
  • Peer Reviewed
• [Journal Article] High prevalence of coinfection with mucosal high-risk type HPV(HR-HPV)and cutaneous HR-HPV in Bowen's disease in the fingers. (2010)
  • Author(s): Nakajima H, Teraishi M, Tarutani M, Sano S.
  • Journal Title: Journal of Dermatological Science Volume: 60(1) Pages: 50-52
  • NAID: 10027473700
  • Peer Reviewed
• [Journal Article] Neutrophil-dominant psoriasis-like skin inflammation induced by epidermal-specific expression of Raf in mice (2010)
  • Author(s): Masahito Tarutani, Yasutomo Imai, Koubun Yasuda, Hiroko Tsutsui, Kenji Nakanishi, Kiyofumi Yamanishi
  • Journal Title: Journal of Dermatological Science 58 Pages: 28-35
  • NAID: 10027400286
  • Peer Reviewed
• [Presentation] Psoriasis-like skin lesion induced by Raf-MAPK signaling requires Stat3 activation (2011)
  • Author(s): Masahito Tarutani
  • Organizer: 36th Annual Meeting of the Japanese Society for Investigative Dermatology
  • Place of Presentation: 京都国際会館
  • Year and Date: 2011-12-10
• [Presentation] Rafの活性化により引き起こされる乾癬様皮疹はStat3シグナルを必要とする (2011)
  • Author(s): 樽谷勝仁,他
  • Organizer: 第26回角化症研究会
  • Place of Presentation: 東京
  • Year and Date: 2011-07-30
• [Presentation] Rafの活性化により引き起こされる乾癬様皮疹はStat3シグナルを必要とする (2011)
  • Author(s): 樽谷勝仁
  • Organizer: 第26回角化症研究会
  • Place of Presentation: 東京商工会議所
  • Year and Date: 2011-07-30
• [Presentation] Psoriasis-like skin lesion induced by Raf-MAPK signaling requires Stat3 activation (2011)
  • Author(s): Masahito Tarutani
  • Organizer: the 71st SID Annual Meeting
  • Place of Presentation: JW Marriott Desert Ridge Resort and Spa (USA)
  • Year and Date: 2011-05-05
• [Presentation] Psoriasis-like skin lesion induced by Raf-MAPK signaling requires Stat3 activation (2011)
  • Author(s): Tarutani M, Miyoshi K, Takaishi M and Sano S
  • Organizer: 36th Annual Meeting of the Japanese Society for Investigative Dermatology
  • Place of Presentation: Kyoto, Japan
• [Presentation] Psoriasis-like skin lesion induced by Raf-MAPK signaling requires Stat3 activation. 71st annual meeting of the society for investigative dermatology, Concurrent Minisymposium 3 (2011)
  • Author(s): Tarutani M, Miyoshi K, Takaishi M and Sano S
  • Organizer: Epidermal Structure and Function
  • Place of Presentation: Phoenix, Arizona, U. S. A.
• [Presentation] 表皮特異的GPHR欠損マウスのphenotype (2010)
  • Author(s): 樽谷勝仁, 他
  • Organizer: 第25回角化症研究会
  • Place of Presentation: 東京商工会議所(東京都)
  • Year and Date: 2010-07-31
• [Presentation] Skin barrier dysfunction with aberrant lamellar bodies in mice lacking keratinocyte-specific Golgi pH regulator (2010)
  • Author(s): Masahito Tarutani, et.al
  • Organizer: The SID 2010 meeting
  • Place of Presentation: Hilton Atlanta Hotel(U.S.A.Atlanta)
  • Year and Date: 2010-05-06
• [Presentation] 乾癬、掌蹠膿疱症の新しい病因論と治療乾癬の病因論2010 (2010)
  • Author(s): 樽谷勝仁
  • Organizer: 第109回日本皮膚科学会総会、教育講演
  • Place of Presentation: 大阪
  • Year and Date: 2010-04-20
• [Presentation] 乾癬の病因論2010 (2010)
  • Author(s): 樽谷勝仁
  • Organizer: 第109回日本皮膚科学会総会
  • Place of Presentation: 大阪国際会議場(大阪市)
  • Year and Date: 2010-04-20
• [Presentation] Neutrophil-dominant psoriasis-like skin inflammation induced by epidermis-specific Raf expression in mice, 39th Annual ESDR Meeting (2009)
  • Author(s): Tarutani M, Imai Y, Tsuda T, Nakanishi K, Yamanishi K
  • Organizer: Poster presentation
  • Place of Presentation: Budapest, Hungary
  • Year and Date: 2009-09-12
• [Presentation] Neutrophil-dominant psoriasis-like skin inflammation induced by epidermal-specific expression of Raf in mice (2009)
  • Author(s): Masahito Tarutani, et.al
  • Organizer: 39th Annual ESDR Meeting
  • Place of Presentation: Semmelweis University(ハンガリー、ブダペスト)
  • Year and Date: 2009-09-12