Vulnerability of Oligodendrocytes to DNA damages-Association with AD-
| Project/Area Number |
21591521
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Psychiatric science
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| Research Institution | Kagawa University |
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Principal Investigator |
NAKAMURA Yuu 香川大学, 医学部, 教授 (70291440)
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| Co-Investigator(Kenkyū-buntansha) |
ITO Koichi 徳島文理大学, 薬学部, 教授 (30291149)
MORI Toshio 奈良県立医科大学, 医学部, 准教授 (10115280)
KUMA Hiromi 香川大学, 医学部附属病院, 助教 (90464362)
亀井 聖史 香川大学, 医学部附属病院, 助教 (60437671)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | オリゴデンドロサイト / NER / DNA損傷修復 / 分化 / 脆弱性 / アストロサイト / 神経細胞 / 無血清培地 / PDGF / NT-3 / UV / 無血清 / 修復 |
|---|
| Research Abstract |
Established primary cultured oligodendrocytes in each stage were exposed to UV(0-10 J/ m^2). Afterwards, they were stained by anti-MBP, GFAP, and 6-4PP antibodies. Fluorescence amount of 6-4PP per fluorescence amount of nuclear DNA was calculated just after UV radiation and at each repair point, and the change of repair activity along with the differentiation stages was studied. NER activity was observed to be changed by differentiation. Furthermore, we investigated the mechanism of the difference of repair activity, and found the difference of the expressions of repair-associated proteins such as PCNA, indicating the difference of the expressions of repair-associated proteins might lead to the difference of repair activity.
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Report
(4 results)
Research Products
(24 results)
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[Journal Article] A 24-week, randomized, double-blind, placebo-controlled study to evaluate the efficacy, safety, and tolerability of the rivastigmine patch in Japanese patients with Alzheimer' s disease2011
Author(s)
Nakamura Y, Fujii A, Graf A, lmai Y, Kim H, Mori J, Shigeta M, Shirahase T, Homma A
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Journal Title
Dement Geriatr Cogn Diord EXTRA
Volume: 1
Pages: 163-179
Related Report
Peer Reviewed
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[Journal Article] A 24-week, randomized, double-blind, placebo-controlled study to evaluate the efficacy, safety, and tolerability of the rivastigmine patch in Japanese patients with Alzheimer's disease2011
Author(s)
Nakamura Y, Fujii A, Graf A, Imai Y, Kim H, Mori J, Shigeta M, Shirahase T, Homma A
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Journal Title
Dement Geriatr Cogn Diord EXTRA
Volume: 1
Pages: 163-179
Related Report
Peer Reviewed
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[Journal Article] Proposed dose equivalence between clonazepam and pramipexole in patients with restless legs syndrome2010
Author(s)
Shinno H, Oka Y, Otsuki M, Tsuchiya S, Mizuno S, Kawada S, Innami T, Sasaki A, Hineno T, Sakamoto T, Inami Y, Nakamura Y, Horiguchi J
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Journal Title
Prog Neuropsychopharmacol Biol Psychiatry
Volume: 34(3)
Pages: 522-526
Related Report
Peer Reviewed
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