The development of conscious mice imaging technique and evaluation of hepatic kinetics of drugs.
| Project/Area Number |
21591547
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Radiation science
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| Research Institution | The University of Tokyo |
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Principal Investigator |
KIRYU Shigeru 東京大学, 医科学研究所, 准教授 (20313124)
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| Co-Investigator(Kenkyū-buntansha) |
INOUE Yusuke 北里大学, 医学部, 教授 (40232566)
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| Co-Investigator(Renkei-kenkyūsha) |
YOSHIKAWA Kohki 駒澤大学, 保健学科, 教授 (10272494)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2010: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2009: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
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| Keywords | マウス / 肝特異性造影剤 / 覚醒 / 体温 / 核磁気共鳴画像(MRI) / 麻酔 |
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| Research Abstract |
The aims of this research were to develop a method for body magnetic resonance imaging(MRI) of conscious mice and to investigate the effect of anesthesia on the hepatic kinetics of drugs. We developed a method for acquiring body MR images in conscious mice by using the plate holder made of polymethylmethacrylate and scotch tape. A conscious mouse was restrained by two operators and the restraint was completed within 3 minutes. The mouse temperature was controlled by circulating water. Serial MRI of the liver was performed after intravenous injection of Gd-EOB-DTPA with or without temperature control. The temperature decreased less in anesthetized mice and remained constant in conscious mice in the radiofrequency coil. The washout of Gd-EOB-DTPA was slower in anesthetized hypothermic mice than in conscious normothermic mice. Warmed anesthetized mice showed faster washout, and cooled conscious mice showed delayed washout. Severer hypothermia in anesthetized mice resulted in weaker initial enhancement and slower washout. By separately manipulating the presence or absence of anesthesia and hypothermia, we demonstrated that washout of Gd-EOB-DTPA was delayed under hypothermia, regardless of anesthesia. We also investigated the effect of drug on Gd-EOB-DTPA kinetics.
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Report
(4 results)
Research Products
(6 results)
