| Project/Area Number |
21591572
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Radiation science
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| Research Institution | Yokohama City University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
INOUE Tomio 横浜市立大学, 医学研究科, 教授 (80134295)
OMURA Motoko 横浜市立大学, 医学研究科, 客員准教授 (70244506)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | siRNA / PET / SPECT / 遺伝子発現イメージング / 99mTc / poly-A-polymelase / 293T細胞 |
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| Research Abstract |
In the experiment using a cultured cell, it was proved that the ^<99m>Tc-labelled siRNA has combined with the specific mRNA about the gene target. Moreover, when improvement of new probe for a PET was tried, we succeeded in optimization of the ^<68>Ga -labelled conditions of siRNA. Furthermore, we succeeded also in labelling siRNA by ^<62>Cu. Interestingly, This radiolabelled siRNA was also able to acquire stability in vivo. However, in the animal experiment, the accumulation to the tumor of the radiolabelled siRNA was low.
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