Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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Research Abstract |
We synthesize N-[^<11> C] methyl-bisindolylmaleimideIII, a selective inhibitor for protein kinase C(PKC), in order to estimate the roll of PKC in the ischemic brain. The radiochemical yield was 30-50%, radiochemical purity was> 95%. The synthetic time was 30 min. Blood clearance was fast and the concentration in blood was low after few minutes. Bio-distributions of this labeled compound showed high uptake in liver and kidney. The uptake in brain was very low. So this tracer was not suitable for estimation of PKC in the ischemic brain.
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