Prediction of radiosensitivity using phosphorylation of histone H2AX in human tumor cell lines

• Project/Area Number: 21591608
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Radiation science
• Research Institution: Juntendo University
• Principal Investigator: SASAI Keisuke 順天堂大学, 医学部, 教授 (20225858)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: リン酸化H2AX / DNA二重鎖切断 / 放射線感受性予測 / DNAプロイディ / 細胞生存率 / アポプトーシス / DNA ploidy / 放射線感受性 / 蛍光免疫染色

Research Abstract

To develop a new predictive assay for radiosensitivity, we tested the relationship between cell survival and expression of phosphorylation of histone H2AX(γH2AX) foci using 7 different human cell lines. There was not a good correlation between the γH2AX foci frequency and surviving fraction after irradiation. When we normalized theγH2AX foci frequency by DNA content, and introduced the frequency of apoptosis to the analysis, there was a good correlation between the cell survival and the combined frequency of apoptosis and γH2AX foci in 6 cell lines except for one.

Research Products

• [Journal Article] The predominant expression of hepatocyte nuclear factor 4α (HNF4α) in thyroid transcription factor-1(TTF-1)-negative pulmonary adenocarcinoma (2011)
  • Author(s): Kunii R, Jiang S, Hasegawa G, Yamamoto T, Umezu H, Watanabe T, Tsuchida M, Hashimoto T, Hamakubo T, Kodama T, Sasai K, Naito M.
  • Journal Title: Histopathology Volume: 58(3) Pages: 467-476
  • Peer Reviewed
• [Journal Article] Effect of dose fractionation on pulmonary complications during total body irradiation (2011)
  • Author(s): Izawa H, Hirowatari H, Yahata Y, Hamano Y, Ito K, Saito AI, Yamamoto H, Miura K, Karasawa K, Sasai K.
  • Journal Title: J Radiat Resarch Volume: 52 Pages: 502-508
  • NAID: 10029122329
  • Peer Reviewed
• [Journal Article] Full-dose capecitabine with loradiotherapy : one of the treatment options for inoperable T4 breast cancer (2011)
  • Author(s): Hirowatari H, Karasawa K, Izawa H, Ito K, Sasai K, Furuya T, Ozawa S, Arakawa A, Orihata G, Saito M.
  • Journal Title: Jpn J Radiol. 2 Volume: 9(3) Pages: 222-225
  • Peer Reviewed
• [Journal Article] Elevation of serumcarcinoembryonic antigen level in a patient with hypothyroidism after radiation therapy for cervical esophageal cancer (2010)
  • Author(s): Kawaguchi G, Abe E, Sasamoto R, Sasai K.
  • Journal Title: Int J Clin Oncol Volume: 15(1) Pages: 104-108
  • NAID: 10027201836
  • Peer Reviewed
• [Journal Article] Long-term outcomes of three-dimensional conformal radiation therapy combined with neoadjuvant hormonal therapy in Japanese patients with locally advanced prostate cancer (2010)
  • Author(s): Sakamoto M, Mizowaki T, Mitsumori M, Takayama K, Sasai K, Norihisa Y, Kamoto T, Nakamura E, Ogawa O, Hiraoka M.
  • Journal Title: Int J Clin Oncol Volume: 15(1) Pages: 104-108
  • NAID: 10027665301
  • Peer Reviewed
• [Journal Article] Radio-sensitization effect poly(ADP-ribose) polymerase inhibition in cells exposed to low and high liner energy transfer radiation
  • Author(s): Hirai T, Shirai H, Fujimori H, Okayasu R, Sasai K, Masutani M.
  • Journal Title: Cancer Science Volume: (in press)
  • Peer Reviewed
• [Journal Article] Effect of dose fractionation on pulmonary complications during total body irradiation.
  • Author(s): H.Izawa, Sasai K, et al
  • Journal Title: J Radiat Research Volume: (印刷中)
  • NAID: 10029122329
  • Peer Reviewed
• [Journal Article] 臨床の立場から放射線増感剤に望むこと
  • Author(s): 笹井啓資
  • Journal Title: がんの臨床 Volume: (印刷中)
• [Presentation] Prediction of radiosensitivity using phosphorylation of histone H2AX (2012)
  • Author(s): Kunogi H, Sasai K
  • Organizer: International Conference on Translational Research in Radio-Oncology and Physics for Health in Europe
  • Place of Presentation: スイスジュネーブ国際会議場
  • Year and Date: 2012-03-01
• [Presentation] Prediction of radiosensitivity using phosphorylation of Histone H2AX (2012)
  • Author(s): Kunogi H, Sasai K.
  • Organizer: Presented at The International Conference on Translational Research in Radio-Oncology and Physics for Health in Europe
  • Place of Presentation: Geneva, Switzerland
• [Presentation] リン酸化H2AXを用いた放射線感受性予測法の基礎研究 (2011)
  • Author(s): 久能木裕明、笹井啓資
  • Organizer: 第70回日本医学放射線学会学術大会(WEB開催)