Prediction of radiosensitivity using phosphorylation of histone H2AX in human tumor cell lines
| Project/Area Number |
21591608
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Radiation science
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| Research Institution | Juntendo University |
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Principal Investigator |
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | リン酸化H2AX / DNA二重鎖切断 / 放射線感受性予測 / DNAプロイディ / 細胞生存率 / アポプトーシス / DNA ploidy / 放射線感受性 / 蛍光免疫染色 |
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| Research Abstract |
To develop a new predictive assay for radiosensitivity, we tested the relationship between cell survival and expression of phosphorylation of histone H2AX(γH2AX) foci using 7 different human cell lines. There was not a good correlation between the γH2AX foci frequency and surviving fraction after irradiation. When we normalized theγH2AX foci frequency by DNA content, and introduced the frequency of apoptosis to the analysis, there was a good correlation between the cell survival and the combined frequency of apoptosis and γH2AX foci in 6 cell lines except for one.
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Report
(4 results)
Research Products
(11 results)
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[Journal Article] The predominant expression of hepatocyte nuclear factor 4α (HNF4α) in thyroid transcription factor-1(TTF-1)-negative pulmonary adenocarcinoma2011
Author(s)
Kunii R, Jiang S, Hasegawa G, Yamamoto T, Umezu H, Watanabe T, Tsuchida M, Hashimoto T, Hamakubo T, Kodama T, Sasai K, Naito M.
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Journal Title
Histopathology
Volume: 58(3)
Pages: 467-476
Related Report
Peer Reviewed
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