Roles of irradiated stromal cells in the survival, growth, differentiation of sqamous cell carcinoma-derived cancer stem cells and its mechanisms研究代表者蒲地紀之(KAMOCHI NORIYUKI)

• Project/Area Number: 21591613
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Radiation science
• Research Institution: Saga University
• Principal Investigator: KAMOCHI Noriyuki 佐賀大学, 医学部, 医学部 (70452836)
• Co-Investigator(Kenkyū-buntansha): TODA Shuji 佐賀大学, 医学部, 医学部 (80188755) ; KUDO Sho 佐賀大学, 医学部, 教授 (50161641)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 扁平上皮癌 / 癌幹細胞 / 線維芽細胞 / 放射線 / 増殖 / 浸潤 / 遺伝子不安定性

Research Abstract

Non-irradiated fibroblasts promote the survival, self-renewal and cancer cell production of cancer stem cells under cancer stem cell-fibroblast interaction, while irradiated fibroblasts tend to promote those of the cancer stem cells. However, both fibroblast types show no significant difference between irradiated and non-irradiated fibroblasts. Finally, the irradiated fibroblasts-cancer stem cell interaction does not induce the significant change of the genomic instability marker p53-binding protein 1(53BP1) expression

Research Products

• [Journal Article] Prolonged effect of fluid flow stress on the proliferative activity of mesothelial cells after abrupt discontinuation of fluid streaming (2011)
  • Author(s): Aoki S, Ikeda S, Takezawa T, Kishi T, Makino J, Uchihashi K, Matsunobu A, Noguchi M, Sugihara H, Toda S
  • Journal Title: Biochem Biophys. Res Commun Volume: 16
  • Peer Reviewed
• [Journal Article] A promising culture model for analyzing the interaction between adipose tissue and cardiomyocytes (2011)
  • Author(s): Anan M, U chihashi K, A oki S, M atsunobu A, Ootani A, Node K, Toda S
  • Journal Title: Endocrinology Volume: 152 Pages: 1599-15609
  • Peer Reviewed
• [Journal Article] Interaction between adipose tissue stromal cells and gastric cancer cells in vitro (2011)
  • Author(s): Nomoto-Kojima N, Aoki S, Uchihashi K, Matsunobu A, Koike E, Ootani A, Yonemitsu N, Fujimoto K, Toda S
  • Journal Title: Cell Tissue Res Volume: 344 Pages: 287-298
  • Peer Reviewed
• [Journal Article] Prolonged effect of fluid flow stress on the proliferative activity of mesothelial cells after abrupt discontinuation of fluid streaming (2011)
  • Author(s): Aoki S, Toda S, et al
  • Journal Title: Biochem Biophys.Res Commun Volume: 16 Issue: 3-4 Pages: 391-396
  • DOI: 10.1016/j.bbrc.2011.11.051
  • Peer Reviewed
• [Journal Article] A promising culture model for analyzing the interaction between adipose tissue and cardiomyocytes (2011)
  • Author(s): Anan M, Toda S, et al
  • Journal Title: Endocrinology Volume: 152 Issue: 4 Pages: 1599-1609
  • DOI: 10.1210/en.2010-1106
  • Peer Reviewed
• [Journal Article] Interaction between adipose tissue stromal cells and gastric cancer cells in vitro. (2011)
  • Author(s): Nomoto-Kojima Namie, Shuji Toda, et al.
  • Journal Title: Cell and Tissue Research Volume: (Published online)
  • Peer Reviewed
• [Journal Article] A Promising Culture Model for Analyzing the Interaction between Adipose Tissue and Cardiomyocytes (2011)
  • Author(s): Mayumi Anan, Shuji Toda, et al
  • Journal Title: Endocrinology Volume: 152 Pages: 1599-1605
  • Peer Reviewed
• [Journal Article] The air liquid-interface, a skin microenvironment, promotes growth of melanoma cells, but not their apoptosis and invasion, through activation of mitogen-activated protein kinase (2010)
  • Author(s): Yee CH, Aoki S, Uchihashi K, M atsunobu A, Yamasaki F, Misago N, Piao M, Tetsuji U, Yonemitsu N, Sugihara H, Toda S
  • Journal Title: Acta Histochem Cytochem Volume: 43 Pages: 1-7
  • NAID: 130000158695
  • Peer Reviewed
• [Journal Article] The air liquid-interface, a skin microenvironment, promotes growth of melanoma cells, but not their apoptosis and invasion, through activation of mitogen-activated proteinkinase. (2010)
  • Author(s): Chong Hong Yee, Shuji Toda, et al.
  • Journal Title: Japan Society of Histochemistry and Cytochemistry Volume: 43(1) Pages: 1-7
  • NAID: 130000158695
  • Peer Reviewed
• [Journal Article] Adipose tissue-organotypic culture system as a promising model for studying adipose tissue biology and regeneration (2009)
  • Author(s): Toda S, Uchihashi K, Aoki S, (他6名)
  • Journal Title: Organogenesis Volume: 5 Pages: 50-56
  • Peer Reviewed
• [Journal Article] Sustained in vitro intestinal epithelial culture within a Wnt-dependent stem cell niche (2009)
  • Author(s): Ootani A, Li X, Sangiorgi E, Ho QT, Ueno H, Toda S, (他5名)
  • Journal Title: Nat Med Volume: 15 Pages: 701-706
  • Peer Reviewed
• [Journal Article] Non-skin mesenchymal cell types support epidermal regeneration in a mesenchymal stem cell or myofibroblast phenotype-independent manner (2009)
  • Author(s): Aoki S, Takezawa T, Uchihashi K, Sugihara H, Toda S
  • Journal Title: Pathol Int Volume: 2009 Pages: 368-375
  • NAID: 10027380333
  • Peer Reviewed
• [Journal Article] Adipose tissue-organotypic culture as a promising model for studying adipose tissue biology and regeneration (2009)
  • Author(s): Shuji Toda, et al.
  • Journal Title: Organogenesis 5 Pages: 50-56
  • Peer Reviewed
• [Journal Article] Sustained in vitro intestinal epithelial culture within a Wnt-dependent stem cell niche (2009)
  • Author(s): Akifumi Ootani, Shuji Toda, et al.
  • Journal Title: Nature Medicine 15 Pages: 701-706
  • Peer Reviewed
• [Journal Article] Non-skin mesenchymal cell types support epidermal regeneration in a mesenchymal stem cell or myofibroblast phenotype-independent manner (2009)
  • Author(s): Shigehisa Aoki, Shuji Toda, et al.
  • Journal Title: Pathology International 59 Pages: 368-375
  • NAID: 10027380333
  • Peer Reviewed