| Project/Area Number |
21591618
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Radiation science
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| Research Institution | Kitasato University |
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Principal Investigator |
KAGIYA Go 北里大学, 医療衛生学部, 講師 (30524243)
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| Co-Investigator(Kenkyū-buntansha) |
OGAWA Ryohei 富山大学, 大学院・医学薬学研究部, 講師 (60334736)
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| Co-Investigator(Renkei-kenkyūsha) |
MATSUMOTO Hideki 福井大学, 高エネルギー医学研究センター, 准教授 (40142377)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
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| Keywords | 低酸素細胞領域 / ニトロキシド化合物 / 殺細胞効果 / HIF-1α / テンポール / 遺伝子発現 / 5-FU / ニトロオキシド化合物 / 遺伝子治療 / 低酸素細胞 |
|---|
| Research Abstract |
We have found that tempol, one of nitoxides, strongly induced the expression of hypoxia inducible-factor(HIF) 1alpha with a combination of hypoxic conditions. The aim of this study is to construct the plasmids that regulate the expression of suicide gene with tempol and to evaluate the enhancement of the cell killing effect by tempol. The transfected cells with the plasmid encoding suicide gene indicated the highest cell killing effect after treatment of 5-FC, tempol and hypoxic mimic CoCl_2.
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