Administration of a hypoxic cell sensitizer, 6-FP, enhance of radiation-induced apoptosis in human carcinoma
| Project/Area Number |
21591619
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Radiation science
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| Research Institution | Keio University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KAWATA Tetsuya 慶應義塾大学, 医学部, 講師 (60234077)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 放射線抵抗性 / 低酸素細胞 / ラジカル / 増感効果 / 染色体解析 / 増感遺伝子解析 / 増感剤 |
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| Research Abstract |
Hypoxia governs cellular adaption to oxygen deficiency by regulating tumor-relevant genes involved in energy metabolism, angiogenesis, cell proliferation and apoptosis. Heterogeneity of tumor was investigated with respect to radiosensitizing efficiencies of a hypoxic cell sensitizer, 6-FP(6-formylpterin), a metabolite of folic acid. In contrast to apoptosis induced by extracellular H_2O_2, only a few studies have examined apoptosis induced by intracellular H_2O_2. Oxidative stress is known to induce many forms of apoptosis, and as its common mediator, intracellular reactive oxygen species play a cardinal role in oxidative stress-induced apoptosis. Here we used an intracellular H_2O_2 inducer, 6-FP, which is a member of the pterin family. Tumor cells and normal cells were treated with 6-FP before expose of X-ray and then irradiated. We collected the cells and colony efficiency and survival fraction were analyzed. The results showed that the tumor cells were higher colony efficiency than the normal cells, and the cells with both X-ray irradiation and 6-FP treatment were significantly lower survival rate. These results suggested that 6-FP may have radioprotective effect for the tumor cells and the incidated that 6-FP enhances the apoptosis by the radiation-induced and 6-FP may be effective use of the drug.
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Report
(4 results)
Research Products
(2 results)
