• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

The mechanism of intimal hyperplasia in vein graft

Research Project

Project/Area Number 21591625
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General surgery
Research InstitutionAsahikawa Medical College

Principal Investigator

UCHIDA Hisashi  旭川医科大学, 医学部, 講師 (60301991)

Co-Investigator(Kenkyū-buntansha) SAITO Yukihiro  旭川医科大学, 医学部, 特任助教 (80540583)
Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords血管平滑筋細胞 / MMP-2 / 内膜肥厚 / 低酸素 / インテグリン / MMP2 / HIF1α / αvβ3インテグリン
Research Abstract

Many investigators have demonstrated that MMPs are important in smooth muscle cell (SMC) migration ex vivo and in neointimal hyperplasia (NH) lesion formation in animal model of injury. This study aim to demonstrate that MMP-2 have a critical role in the early NH lesion formation after arteriovenous (AV) access creation. Methods and Results : To determine MMP-2 is critical to early NH lesion formation after AV access creation, we created an AV fistula in mice by anastomosing the common carotid artery to the external jugular vein in an end-to-side fashion. After 3 weeks, MMP-2-/-mice have significantly less anastomotic NH lesion formation compared to the wild-type MMP-2+/+ mice confirming that MMP-2 activity is indeed critical to early neointimal hyperplasia in the setting of AV fistulae. And there is significant increase in the tissue levels of pro-MMP-2 and activated MMP-2 based the gelatin-substrate zymography. Also foundMMP-2-/-micehad noMMP-2 activityfonollowing AV fistula creation. These MMP-2-/-wemice did not have compensatory increase in the expression of MMP-9. We also demonstrated that like the human counterpart, the mouse NH lesion is predominantly SMCs (SM specific α-actin immunostaining) with minimal number of macrophages (Mac-3 immunostaining). In vitro, integrin αv, β3, and MMP-2 was up-regulated in SMC, when the cells cultured under hypoxic conditions. It seemed to have some relations between these molecules at its expressions. Conclusions : Ewasarly primary AV access failure due to anastomotic neointimal hyperplasia is associated with increased MMP-2-dependent SMC migration. This association is confirmed with the AV fistula mouse model, which demonstrated a significantly less anastomotic neointimal lesion formation in MMP-2-/-mice as compared to MMP-2+/+ mice.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (13 results)

All 2012 2011 2010 2009

All Journal Article (5 results) Presentation (7 results) Book (1 results)

  • [Journal Article] 血管平滑筋細胞を主体とする動静脈シャント吻合部内膜肥厚形成とマトリクスメタロプロテアーゼ2発現の関与2012

    • Author(s)
      内田恒
    • Journal Title

      北海道医学雑誌

      Volume: vol87 Pages: 115-120

    • NAID

      10030576058

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Journal Article] Overview-わが国における末梢動脈疾患血行再建術2011

    • Author(s)
      内田恒、笹嶋唯博
    • Journal Title

      Heart View

      Volume: vol.4 Pages: 41-46

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Journal Article] ハイブリッド手術室の有用性を血管外科の視点から検証する2011

    • Author(s)
      内田恒
    • Journal Title

      月刊新医療

      Volume: vol.442 Pages: 99-101

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Journal Article] Overview-わが国における末梢動脈疾患血行再建術2011

    • Author(s)
      内田恒
    • Journal Title

      月刊Heart View 2011年4月号特集

      Volume: 4月号 Pages: 41-46

    • Related Report
      2010 Annual Research Report
  • [Journal Article] [Expertise]腹部大動脈閉塞に対する血管内治療の可否は?2010

    • Author(s)
      内田恒
    • Journal Title

      刊Heart View 9月号

      Volume: 9月号 Pages: 106-111

    • Related Report
      2010 Annual Research Report
  • [Presentation] EVAR 93例の初期-中期成績(IFU vs IFU外症例)2011

    • Author(s)
      内田恒
    • Organizer
      第51回日本脈管学会総会(ポスター)
    • Place of Presentation
      岐阜
    • Year and Date
      2011-10-20
    • Related Report
      2011 Annual Research Report
  • [Presentation] ANASTOMOTIC NEOINTIMAL HYPERPLASIA AS RELATED TO ARTERIOVENOUS ACCESS IS ASSOCIATED WITH MMP-2 DEPENDENT SMOOTH MUSCLE CELL MOTILITY2011

    • Author(s)
      内田恒
    • Organizer
      12^<th> ASVS(アジア血管外科学会)oral presentation
    • Place of Presentation
      台北(台湾)
    • Year and Date
      2011-09-30
    • Related Report
      2011 Annual Research Report
  • [Presentation] 大腿動脈以下末梢動脈病変に対する治療戦略2011

    • Author(s)
      内田恒
    • Organizer
      第38回日本血管外科学会総会(シンポジウム)
    • Place of Presentation
      沖縄
    • Year and Date
      2011-05-21
    • Related Report
      2011 Annual Research Report
  • [Presentation] ハイブリORの現状と未来(ランチョンセミナー)2011

    • Author(s)
      内田恒
    • Organizer
      第41回日本心臓血管外科学会総会
    • Place of Presentation
      浦安市
    • Year and Date
      2011-02-25
    • Related Report
      2010 Annual Research Report
  • [Presentation] Long-term results after bypass surgery in patients with intermittent claudication.(シンポジウム)2011

    • Author(s)
      内田恒
    • Organizer
      第41回日本心臓血管外科学会総会
    • Place of Presentation
      浦安市
    • Year and Date
      2011-02-24
    • Related Report
      2010 Annual Research Report
  • [Presentation] 頸動脈狭窄病変に対するCEA治療成績(パネルディスカッション1)2010

    • Author(s)
      内田恒
    • Organizer
      第38回日本血管外科学会総会
    • Place of Presentation
      名古屋市
    • Year and Date
      2010-05-21
    • Related Report
      2010 Annual Research Report
  • [Presentation] Arguments of"Open First"procedure for critical limb ischemia2010

    • Author(s)
      内田恒
    • Organizer
      日本循環器学会2010
    • Place of Presentation
      京都市
    • Year and Date
      2010-03-06
    • Related Report
      2009 Annual Research Report
  • [Book] EVTテクニック これは困ったどうしよう!大腿膝窩動脈領域に対する治療戦略、膝下膝窩動脈領域に対する治療戦略2009

    • Author(s)
      内田恒
    • Total Pages
      438
    • Publisher
      中外医学社
    • Related Report
      2009 Annual Research Report

URL: 

Published: 2009-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi