Attenuation of Immunological Responses Against Islet Transplants by Using Polyphenols

• Project/Area Number: 21591636
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General surgery
• Research Institution: Kyoto University
• Principal Investigator: IWANAGA Yasuhiro 京都大学, 医学研究科, 助教 (80378661)
• Co-Investigator(Kenkyū-buntansha): UEMOTO Shinji 京都大学, 医学研究科, 教授 (40252449) ; 金宗 潤 京都大学, 医学研究科, 研究員 (10511925)
• Co-Investigator(Renkei-kenkyūsha): HYON S-h 京都大学, 再生医科学研究所, 准教授 (90283655)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 膵島移植 / 免疫抑制 / 拒絶反応 / ポリフェノール / 糖尿病 / 抗原提示・認識 / 免疫カムフラージュ / アナジー / GVHD

Research Abstract

We evaluated the immunological attenuation effects of a polyphenol(epigallocatechin gallate=EGCG) by mixed lymphocyte reaction assays and murine islet transplantation models. EGCG attenuated immunological reactions in allograft transplantations for both immunological rejection and graft-versus-host disease. In the rejection model, EGCG blocked CD28 and CD3_ε, rendering incomplete stimulation and subsequent apoptosis in the responder cells. EGCG by blocking similar co-stimulatory signals also attenuated the severity of GVHD. Taken together, EGCG was found to exhibit immunological attenuating effects and be a potential model compound for developing immune-suppression drugs in transplantation medicine.

Research Products

• [Journal Article] Attenuation of murine GVHD by a tea polyphenol (2012)
  • Author(s): Kanamune, J., Iwanaga, Y., Kina, T., Noguchi, H., Matsumura, K., Uemoto, S., Hyon, S-H.
  • Journal Title: Cell Transplantation Volume: (In press) Issue: 5 Pages: 909-918
  • DOI: 10.3727/096368911x623934
  • Peer Reviewed
• [Journal Article] 膵島移植における免疫抑制法 (2011)
  • Author(s): 岩永康裕、金宗潤、高折恭一、上本伸二
  • Journal Title: 膵臓 Volume: 26 Pages: 197-203
  • NAID: 10029435767