The enhancement of therapeutic efficacy of autologous cells by hypoxic preconditioning

• Project/Area Number: 21591640
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General surgery
• Research Institution: Yamaguchi University
• Principal Investigator: KATSURA Syunsaku 山口大学, 医学部附属病院, 助教 (40457304)
• Co-Investigator(Kenkyū-buntansha): HAMANO Kimikazu 山口大学, 大学院・医学系研究科, 教授 (60263787) ; KUBO Masayuki 山口大学, 大学院・医学系研究科, 助教 (60420519)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 血管新生 / 細胞移植 / 生着率 / 低酸素 / プレコンディショニング / 再生医療 / 末梢血単核球

Research Abstract

Therapeutic angiogenesis by the implantation of autologous cells is a promising treatment for ischemic diseases. However, the poor retention of the cells after implantation into ischemic tissue is a major problem that related to poor potential of cell-based therapy. This study shows that hypoxic preconditioning enhances the therapeutic potential of implanted cells by increasing their retention. This approach is a short-term and simple method of enhancing the angiogenic potency of implanted cells. Further clinical trials are required to confirm the efficiency of this method.

Research Products

• [Journal Article] Hypoxic preconditioning enhances angiogenic potential of bone marrow cells with aging-related functional impairment (2012)
  • Author(s): Kubo M, Li TS, Kurazumi H, Takemoto Y, Ohshima M, Murata T, Katsura S, Morikage N, Furutani A, Hamano K
  • Journal Title: Circ J Volume: 76(4) Pages: 986-994
  • NAID: 10030131630
  • Peer Reviewed
• [Journal Article] Hypoxic preconditioning enhances angiogenic potential of bone marrow cells with aging-related functional impairment (2012)
  • Author(s): Masayuki Kubo
  • Journal Title: Circ J Volume: (in press)
  • NAID: 10030131630
  • Peer Reviewed
• [Journal Article] Increased expression of CXCR4 and integrin M in hypoxia-preconditioned cells contributes to improved cell retention and angiogenic potency (2009)
  • Author(s): Kubo M, Li TS, Kamota T, Ohshima M, Qin SL, Hamano K
  • Journal Title: J Cell Physiol Volume: 220(2) Pages: 508-514
  • Peer Reviewed
• [Journal Article] Increased expression of CXCR4 and integrin α M in hypoxia-preconditioned cells contributes to improved cell retention and angiogenic potency (2009)
  • Author(s): Masayuki Kubo
  • Journal Title: Journal of Cellular Physiology 220 Pages: 508-514
  • Peer Reviewed
• [Presentation] Hypoxic preconditioning enhances the angiogenic and therapeutic potential of bone marrow cells with age-related functional impairment (2011)
  • Author(s): Kubo M, Li TS, Kurazumi H, Takemoto Y, Ohshima M, Hamano K
  • Organizer: American Heart Association Scientific Sessions 2011
  • Place of Presentation: Orange County Convention Centre(オーランド、アメリカ)
  • Year and Date: 2011-11-14
• [Presentation] Hypoxic preconditioning enhances the angiogenic and therapeutic potential of bone marrow cells with age-related functional impairment (2011)
  • Author(s): Masayuki Kubo
  • Organizer: American Heart Association Scientific Sessions 2011
  • Place of Presentation: Orange County convention Centre(オーランド,アメリカ)
  • Year and Date: 2011-11-14
• [Presentation] 骨髄由来細胞を用いた血管再生治療に対する低酸素プレコンディショニング (2011)
  • Author(s): 藏澄宏之, 久保正幸, 大島真子, 李桃生, 濱野公一
  • Organizer: 第11回心血管再生先端治療フォーラム
  • Place of Presentation: 品川グランドセントラルタワー(東京都)
  • Year and Date: 2011-07-02
• [Presentation] 低酸素プレコンディショニングによる移植細胞の機能増強に基づいた血管再生治療法の開発 (2011)
  • Author(s): 久保正幸李桃生, 藏澄宏之, 森景則保, 美甘章仁, 濱野公一
  • Organizer: 日本循環器学会第98回中国・四国合同地方会
  • Place of Presentation: あわぎんホール(徳島県)
  • Year and Date: 2011-05-03
• [Presentation] 加齢動物の骨髄細胞に対する低酸素プレコンディショニングの有用性:細胞移植治療の効果向上を目指して (2010)
  • Author(s): 久保正幸, 李桃生, 大島真子, 桂春作, 古谷彰, 濱野公一
  • Organizer: 第9回日本再生医療学会総会
  • Place of Presentation: 広島国際会議場(広島県)
  • Year and Date: 2010-03-18
• [Presentation] 加齢動物の骨髄細胞に対する低酸素プレコンディショニングの有用性:細胞移植治療の効果向上を目指して (2010)
  • Author(s): 久保正幸
  • Organizer: 第9回日本再生医療学会総会
  • Place of Presentation: 広島
  • Year and Date: 2010-03-18
• [Presentation] Hypoxic pretreatment improves age-related functional impairment of bone marrow cells for inducing therapeutic angiogenesis (2009)
  • Author(s): 久保正幸, 李桃生, 大島真子, 桂春作, 古谷彰, 濱野公一
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2009-12-09
• [Presentation] Hypoxic pretreatment improves age-related functional impairment of bone marrow cells for inducing therapeutic angiogenesis (2009)
  • Author(s): 久保正幸
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-09