Analysis of a novel BRCA1-associated protein for new cancer therapy

• Project/Area Number: 21591656
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General surgery
• Research Institution: Tohoku University
• Principal Investigator: CHIBA Natsuko 東北大学, 加齢医学研究所, 准教授 (50361192)
• Co-Investigator(Renkei-kenkyūsha): KANNO Shin-ichiro 東北大学, 加齢医学研究所, 講師 (10400417)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 乳癌 / 化学療法 / 標的分子 / バイオマーカー / がん / BRCA1 / 細胞分裂制御 / DNA修復

Research Abstract

Germline mutations in the breast cancer susceptibility gene BRCA1 predispose women to breast and ovarian cancers. BRCA1 plays important roles in DNA repair and centrosome regulation, along with its heterodimer partner BARD1. We identified a new protein that interacts with BARD1 (here we call it BARD1-interacting protein (BIP)). We found that BIP plays an important role in the regulation of centrosome amplification and cytokinesis together with BRCA1and BARD1.

Research Products

• [Journal Article] Functional differences among BRCA1 missense mutations in the control of centrosome duplication. (2012)
  • Author(s): Kais Z, Chiba N, Ishioka C, Parvin J D
  • Journal Title: Oncogene Volume: 31(6) Issue: 6 Pages: 799-804
  • DOI: 10.1038/onc.2011.271
  • Peer Reviewed
• [Journal Article] Analysis of Tumor Suppressor BRCA1 using Molecular Imaging (2012)
  • Author(s): Natsuko Chiba
  • Journal Title: Tohoku University Global COE Program, Global Nano-Biomedical Engineering Education and Research Network Centre, Annals of nanoBME Volume: Vol.5 Pages: 211-220
• [Journal Article] 個別化医療のための分子イメージング技術を用いた癌関連分子の機能解析 (2012)
  • Author(s): 千葉奈津子
  • Journal Title: 東北大学グローバルCOEプログラム新世紀世界の成長焦点に築くナノ医工学拠点ナノ医工学年報 Volume: Vol.5 Pages: 231-240
  • NAID: 40019250290
• [Journal Article] BRCA1 contributes to transcription-coupled repair of DNA damage through polyubiquitination and degradation of Cockayne syndrome B protein. (2011)
  • Author(s): Wei L, Lan L, Yasui A, Tanaka K, Saijo M, Matsuzawa A, Kashiwagi R, Maseki E, Hu Y, Parvin J D, Ishioka C and Chiba N
  • Journal Title: Cancer Science Volume: 102(10) Issue: 10 Pages: 1840-7
  • DOI: 10.1111/j.1349-7006.2011.02037.x
  • NAID: 10030243954
  • Peer Reviewed
• [Journal Article] Identifying the effect of BRCA1 mutations on homologous recombination using cells that express endogenous wild-type (2011)
  • Author(s): Parvin J D, Chiba N, and Ransburgh D
  • Journal Title: BRCA1 Volume: 48 Issue: 48
  • DOI: 10.3791/2468
  • URL: http://www.jove.com/index/Details.stp?ID=2468J.Vis.Exp
  • Peer Reviewed
• [Journal Article] Identification of breast tumor mutations in BRCA1 that abolish its function in homologous DNA recombination. (2010)
  • Author(s): Ransburgh D, Chiba N, Ishioka C, Toland A, and Parvin J D
  • Journal Title: Cancer Research Volume: 70(3) Pages: 988-95
  • URL: http://www.ncbi.nlm.nih.gov/pubmed/20103620
  • Peer Reviewed
• [Journal Article] KSRP/FUBP2 is a binding protein of GO-Y086, a cytotoxic curcumin analogue (2010)
  • Author(s): Hiroyuki Yamakoshi
  • Journal Title: ACS Medicinal Chemistry Letters Volume: 1(6) Pages: 273-276
  • Peer Reviewed
• [Journal Article] Identifying the effect of BRCA1 mutations on homologous recombination using cells that express endogenous wild-type BRCA1 (2010)
  • Author(s): Jeffrey D.Parvin
  • Journal Title: Journal of Visualized Experiments Volume: 48
  • Peer Reviewed
• [Journal Article] 個別化医療のための分子イメージング技術を用いた癌抑制遺伝子の機能解析 (2010)
  • Author(s): 千葉奈津子
  • Journal Title: 東北大学グローバルCOEプログラム 新世紀世界の成長焦点に築くナノ医工学拠点 ナノ医工学年報 Volume: Vol.4 Pages: 222-231
  • NAID: 40017238541
• [Journal Article] 乳癌の予防と治療のための大豆イソフラボンと家族性乳癌原因遺伝子BRCA1の関連の解明 (2010)
  • Author(s): 柏木梨佐
  • Journal Title: 大豆たん白質研究 Volume: Vol.13 Pages: 120-124
• [Journal Article] The effect of BRCA1 missense mutations on homology directed recombination (2010)
  • Author(s): Derek J.R.Ransburgh
  • Journal Title: Cancer Research 70(3) Pages: 988-995
  • Peer Reviewed
• [Journal Article] Phase I/II study of sequential therapy with irinotecan and S-1 for metastatic colorectal cancer (2009)
  • Author(s): Takashi Yoshioka
  • Journal Title: British Journal of Cancer. 101(12) Pages: 1972-1977
  • Peer Reviewed
• [Journal Article] Analysis of Tumor Suppressor Gene Using Molecular Imaging for Personalized Medicine (2009)
  • Author(s): 千葉奈津子
  • Journal Title: Tohoku University Global COE Program, Global Nano-Biomedical Engineering Education and Research Network Centre, Annals of nanoBME Vol.3 Pages: 209-218
• [Journal Article] 個別化医療のための分子イメージング技術を用いた癌抑制遺伝子の機能解析 (2009)
  • Author(s): 千葉奈津子
  • Journal Title: 東北大学グローバルCOEプログラム 新世紀世界の成長焦点に築くナノ医工学拠点 ナノ医工学年報 Vol.3 Pages: 211-220
  • NAID: 40017238541
• [Journal Article] (2009)
  • Author(s): Natsuko Chiba
  • Journal Title: Proceeding of the Tohoku University Global Centre of Excellence Programme, Global Nano-Biomedical Engineering Education and Research Network Centre(Imperial College Press) Pages: 339-349
• [Journal Article] (2009)
  • Author(s): Leizhen Wei
  • Journal Title: Proceeding of the Tohoku University Global Centre of Excellence Programme, Global Nano-Biomedical Engineering Education and Research Network Centre(Imperial College Press) Pages: 459-465
• [Presentation] Analysis of Tumor Suppressor BRCA1 using Molecular Imaging. (2012)
  • Author(s): Chiba N, Furukawa Y, Shibata S, Kato K, Mochiduki H, Shiono M, Maseki E, and Matsuzawa A
  • Organizer: The 18th International Symposium on Nano-Biomedical
  • Place of Presentation: Engineering in the East Asian-Pacific Rim Region (Sendai, Japan)
  • Year and Date: 2012-03-06
• [Presentation] Analysis of Tumor Suppressor BRCA1 using Molecular Imaging (2012)
  • Author(s): Natsuko Chiba
  • Organizer: The 18^<th> International Symposium on Nano-Biomedical Engineering in the East Asian-Pacific Rim Region
  • Place of Presentation: Sendai
  • Year and Date: 2012-03-06
• [Presentation] A novel BARD1-interacting protein regulates centrosome (2012)
  • Author(s): Ayako Matsuzawa
  • Organizer: The 18^<th> International Symposium on Nano-Biomedical Engineering in the East Asian-Pacific Rim Region
  • Place of Presentation: Sendai
  • Year and Date: 2012-03-06
• [Presentation] The regulatory mechanism of BRCA1 and BARD1 after DNA damage (2012)
  • Author(s): Emiko Maseki
  • Organizer: The 18^<th> International Symposium on Nano-Biomedical Engineering in the East Asian-Pacific Rim Region
  • Place of Presentation: Sendai
  • Year and Date: 2012-03-06
• [Presentation] A novel BARD1-interacting protein regulates centrosome and cytokinesis. (2011)
  • Author(s): Matsuzawa A, Shibata S, Mochiduki H, Maseki E, , Furukawa Y, Kato K, Shiono M, and Chiba N
  • Organizer: 5th East Asian Pacific Student Workshop on Nano-Biomedical Engineering
  • Place of Presentation: National University of Singapore, Singapore, Singapore
  • Year and Date: 2011-12-12
• [Presentation] Regulation of the level of BRCA1/BARD1 expression. (2011)
  • Author(s): Maseki E, Shiono M, Matsuzawa A, Furukawa Y, Shibata S, Mochiduki H, Kato K, and Chiba N
  • Organizer: 5th East Asian Pacific Student Workshop on Nano-Biomedical Engineering
  • Place of Presentation: National University of Singapore, Singapore, Singapore
  • Year and Date: 2011-12-12
• [Presentation] Prediction of colorectal cancer prognosis by gene expression profile using FFPE sample (2011)
  • Author(s): Masahiro Inoue
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 名古屋
  • Year and Date: 2011-10-04
• [Presentation] SNPs of Aurora-A are involved in the centrosome regulation of BRCA1 and malignant potential of esophageal cancer. Aurora-Aの一塩基多型はBRCA1による中心体制御と食道癌の悪性度に関与する。 (2011)
  • Author(s): Matsuzawa A, Mori T, Mochiduki H, Chiba N
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 名古屋
  • Year and Date: 2011-10-03
• [Presentation] SNPs of Aurora-A are involved in the centrosome regulation of BRCA1 and malignant potential of esophageal cancer (2011)
  • Author(s): Ayako Matsuzawa
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 名古屋
  • Year and Date: 2011-10-03
• [Presentation] Function of BRCA1 in DNA damage repair and centrosome regulation. (2011)
  • Author(s): Chiba N, Matsuzawa A, Kashiwagi R, Maseki E, Kanno S, Wei L, Furukawa Y, Shibata S, Ishioka C, and Yasui A
  • Organizer: The 6th International Symposium of Institute Network
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 2011-06-09
• [Presentation] Identification of a novel BARD1-interacting protein and analyses of its function. (2010)
  • Author(s): Matsuzawa A, Kanno S, Wei L, Kashiwagi R, Maseki E, Shibata S, Furukawa Y, Ishioka C, Yasui A, and Chiba N
  • Organizer: 4th East Asian Pacific Student Workshop on Nano-Biomedical Engineering
  • Place of Presentation: National University of Singapore, Singapore, Singapore
  • Year and Date: 2010-12-16
• [Presentation] Regulatory mechanism of the level of BRCA1/BARD1 expression following DNA damage. (2010)
  • Author(s): aseki E, Kanno S, Matsuzawa A, Kashiwagi R, Furukawa Y, Shibata S, Yasui A, and Chiba N
  • Organizer: 4th East Asian Pacific Student Workshop on Nano-Biomedical Engineering
  • Place of Presentation: National University of Singapore, Singapore, Singapore
  • Year and Date: 2010-12-16
• [Presentation] Identification of a novel BARD1-interacting protein and analyses of its function. (2010)
  • Author(s): Ayako Matsuzawa
  • Organizer: 4th East Asian Pacific Student Workshop on Nano-Biomedical Engineering
  • Place of Presentation: Singapore, Singapore
  • Year and Date: 2010-12-16
• [Presentation] Regulatory mechanism of the level of BRCA1/BARD1 expression following DNA damage. (2010)
  • Author(s): Emiko Maseki
  • Organizer: 4th East Asian Pacific Student Workshop on Nano-Biomedical Engineering
  • Place of Presentation: Singapore, Singapore
  • Year and Date: 2010-12-16
• [Presentation] BRCA1はDNA単鎖切断修復に関与する (2010)
  • Author(s): 柏木梨佐、古川裕美子、宇井彩子、松澤綾子、柵木絵美子、柴田峻、石岡千加史、安井明、千葉奈津子
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-08
• [Presentation] Regulatory mechanism of the level of BRCA1/BARD1 expression following DNA damage (2010)
  • Author(s): Maseki E, Kanno S, Matsuzawa A, Kashiwagi R, Shibata S, Furukawa Y, Wei L, Ishioka C, Yasui A, and Chiba N
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-08
• [Presentation] Identification of a novel BARD1-interacting protein that regulates centrosome and cytokinesis中心体と細胞質分裂を制御する新規BARD1結合分子の同定 (2010)
  • Author(s): Matsuzawa A, Wei L, Kashiwagi R, Maseki E, Ishioka C, Yasui A, and Chiba N
  • Organizer: 第69回日本癌学会学術総会
  • Place of Presentation: 大阪
  • Year and Date: 2010-09-24
• [Presentation] Identification of a novel BARD1-interacting protein that regulates centrosome and cytokinesis (2010)
  • Author(s): Ayako Matsuzawa
  • Organizer: 第69回日本癌学会学術総会
  • Place of Presentation: 大阪
  • Year and Date: 2010-09-24
• [Presentation] The effect of BRCA1 missense mutations on homology directed recombination, BRCA1の点突然変異の相同組み換えへの影響 (2010)
  • Author(s): Chiba N, Matsuzawa A, Kashiwagi R, Maseki E and Ishioka C
  • Organizer: 第69回日本癌学会学術総会
  • Place of Presentation: 大阪
  • Year and Date: 2010-09-22
• [Presentation] The effect of BRCA1 missense mutations on homology directed recombination (2010)
  • Author(s): Natsuko Chiba
  • Organizer: 第69回日本癌学会学術総会
  • Place of Presentation: 大阪
  • Year and Date: 2010-09-22
• [Presentation] A novel BARD1-interacting protein functions at cell cycle regulation. (2010)
  • Author(s): Matsuzawa A, Kanno S, Wei L, Kashiwagi R, Maseki E, Ishioka C, Yasui A, and Chiba N
  • Organizer: 6th World Congress of Biomechanics
  • Place of Presentation: Suntec City, Singapore, Singapore
  • Year and Date: 2010-08-03
• [Presentation] Regulation of BRCA1/BARD1 expression after DNA damage. (2010)
  • Author(s): Maseki E, Kanno S, Matsuzawa A, Kashiwagi R, Wei L, Ishioka C, Yasui A, and Chiba N
  • Organizer: 6th World Congress of Biomechanics
  • Place of Presentation: Suntec City, Singapore, Singapore
  • Year and Date: 2010-08-03
• [Presentation] A novel BARD1-interacting protein functions at cell cycle regulation. 6th World Congress of Biomechanics (2010)
  • Author(s): Ayako Matsuzawa
  • Organizer: 6th World Congress of Biomechanics
  • Place of Presentation: Singapore, Singapore
  • Year and Date: 2010-08-03
• [Presentation] Regulation of BRCA1/BARD1 expression after DNA damage. (2010)
  • Author(s): Emiko Maseki
  • Organizer: 6th World Congress of Biomechanics
  • Place of Presentation: Singapore, Singapore
  • Year and Date: 2010-08-03
• [Presentation] Analysis of tumor suppressor gene using Molecular imaging technique for paesonalized medicine (2010)
  • Author(s): 千葉奈津子
  • Organizer: The 12^<th> Internatopnal Symposium on Nano-Biomedical Engineering in the East Asian-Pacific Rim Region
  • Place of Presentation: Sendai, Japan
  • Year and Date: 2010-04-16
• [Presentation] Analysis of tumor suppressor gene using Molecular imaging technique for personalized medicine. (2010)
  • Author(s): Chiba N, Kashiwagi R, Maseki E, and Matsuzawa A
  • Organizer: The 12th Internatopnal Symposium on Nano-Biomedical Engineering in the East Asian-Pacific Rim Region
  • Place of Presentation: Sendai, Japan
  • Year and Date: 2010-03-26
• [Presentation] A novel BARD1-interacting protein functions at spindle poles and midbody to regulate mitosis and cytokinesis. (2009)
  • Author(s): 松澤綾子、菅野新一郎、魏雷震、柏木梨佐、柵木絵美子、石岡千加史、安井明、千葉奈津子
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-11
• [Presentation] A novel BARD1-interacting protein functions at spindle poles and midbody to regulate mitosis and cytokinesis (2009)
  • Author(s): 松澤綾子
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-11
• [Presentation] BRCA1のコケイン症候群CSB蛋白のユビキチン化と転写共役修復への影響 (2009)
  • Author(s): 千葉奈津子、魏雷震、田中亀代次、石岡千加史、安井明
  • Organizer: 第20回DNA複製・組み換え・修復ワークショップ
  • Place of Presentation: 滋賀県彦根市
  • Year and Date: 2009-11-01
• [Presentation] BRCA1のDNA単鎖切断修復能における機能 (2009)
  • Author(s): 柏木梨佐、魏雷震、松澤綾子、柵木絵美子、石岡千加史、安井明、千葉奈津子
  • Organizer: 第20回DNA複製・組み換え・修復ワークショップ
  • Place of Presentation: 滋賀県彦根市
  • Year and Date: 2009-11-01
• [Presentation] BRCA1 polyubiquitinates CSB protein in transcription-coupled repair of UV damage、転写と共役したDNA修復においてBRCA1がコケイン症候群CSB蛋白をユビキチン化する (2009)
  • Author(s): 千葉奈津子, 魏雷震, 田中亀代次, 安井明, 石岡千加史
  • Organizer: 第68回日本癌学会学術総会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-10-03
• [Presentation] BRCA1 polyubiquitinates CSB protein in transcription-coupled repair of UV damage (2009)
  • Author(s): 千葉奈津子
  • Organizer: 第68回日本癌学会学術総会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-10-03
• [Presentation] Identification of a new target of cancer therapy by a proteomic study. The 10th Internatopnal Symposium on Nano-Biomedical Engineering (2009)
  • Author(s): Chiba N, Matsuzawa A, Wei L, Kashiwagi R, Maseki E, Yasui A, and Kanno S
  • Organizer: in the East Asian-Pacific Rim Region
  • Place of Presentation: Christchurch, New Zealand
  • Year and Date: 2009-04-16
• [Book] BRCA1 is involved in the transcription-coulpled repair of UV lesions. Nano-Biomedical Engineering 2011 (2012)
  • Author(s): Chiba N and Wei L
  • Publisher: Imperial College Press
• [Book] Regulation of BRCA1 and BARD1 expression levels in Response to DNA damageNano-Biomedical Engineering 2011 (2012)
  • Author(s): Maseki E, Shiono M, Matsuzawa A, Wei L, Kashiwagi R, Furukawa Y, Shibata S, Mochiduki H, Kato K, and Chiba N.
  • Publisher: Imperial College Press
• [Book] Identification of a novel BARD1-interacting protein and an analysis of its function in the regulation of mitosis. Nano-Biomedical Engineering 2011 (2012)
  • Author(s): Matsuzawa A, Wei L, Kashiwagi R, Shibata S, Mochiduki H, Maseki E, Furukawa Y, Kato K, Shiono M, and Chiba N.
  • Publisher: Imperial College Press
• [Book] BRCA1 responds to DNA damage induced by laser-irradiation. Nano-Biomedical Engineering 2011 (2012)
  • Author(s): Wei L, Kashiwagi R, Furukawa Y, Kato K, and Chiba N.
  • Publisher: Imperial College Press
• [Book] Nano-Biomedical Engineering 2011, Proceeding of the Tohoku University Global Centre of Excellence Programme, Global Nano-Biomedical Engineering Education and Research Network Centre (2012)
  • Author(s): Natsuko Chiba
  • Publisher: Imperial College Press
• [Book] Nano-Biomedical Engineering 2011, Proceeding of the Tohoku University Global Centre of Excellence Programme, Global Nano-Biomedical Engineering Education and Research Network Centre (2012)
  • Author(s): Emiko Maseki
  • Publisher: Imperial College Press
• [Book] Nano-Biomedical Engineering 2011, Proceeding of the Tohoku University Global Centre of Excellence Programme, Global Nano-Biomedical Engineering Education and Research Network Centre (2012)
  • Author(s): Ayako Matsuzawa
  • Publisher: Imperial College Press
• [Book] Nano-Biomedical Engineering 2011, Proceeding of the Tohoku University Global Centre of Excellence Programme, Global Nano-Biomedical Engineering Education and Research Network Centre (2012)
  • Author(s): Leizhen Wei
  • Publisher: Imperial College Press
• [Book] Analysis of Tumor Suppressor Gene Using Molecular Imaging for Personalized Medicine, Nano-Biomedical Engineering 2009 (2009)
  • Author(s): Chiba N and Wei L
  • Publisher: Imperial College Press
• [Book] Analysis of BRCA1 Accumulation at DNA Double-Strand Breaks Using a Molecular Imaging technique, Nano-Biomedical Engineering 2009 (2009)
  • Author(s): Wei L and Chiba N
  • Publisher: Imperial College Press
• [Remarks]
  • URL: http://www.lifesci.tohoku.ac.jp/research/field_list/neurogenetics/ts_nchiba