Research and development of cure methods for the breast cancer by expression revel controlling of glycosyltransferases

• Project/Area Number: 21591667
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General surgery
• Research Institution: Kyoto Institute of Technology (2009, 2011); Osaka University (2010)
• Principal Investigator: KONDO Akihiro 京都工芸繊維大学, 遺伝資源キュレーター教育研究センター, 特任教授 (60359841)
• Co-Investigator(Renkei-kenkyūsha): NAKAGAWA Takatoshi 大阪医科大学, 医学部, 助教 (70359842)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 乳腺外科

Research Abstract

We had found out that make a fucosyltransferase VIII(FUT8) gene knock down and multiplication of TGP-49 cells or HepG2 cells were controlled by using the technique of RNAi in the experiment which used TGP-49 cells and HepG2 cells. It has checked that applied this method to a breast cancer cell(MCF-7), verified whether down regulation control of a breast cancer cell multiplication is possible by making FUT8 gene knock down, and multiplication of MCF-7 was controlled as expected by this research.

Research Products

• [Journal Article] Core fucosylation ofμheavy chains regulates assembly and intracellular signaling of precursor B cell receptors (2012)
  • Author(s): Li W, Liu Q, Pang Y, Jin J, Wang H, Cao H, Li Z, Wang X, Ma B, Chi Y, Wang R, Kondo A, Gu J, Taniguchi N
  • Journal Title: J Biol Chem Volume: 287(4) Pages: 2500-8
  • Peer Reviewed
• [Journal Article] Core fucosylation of μ heavy chains regulates assembly and intracellular signaling of precursor B cell receptors (2012)
  • Author(s): Li W, 他
  • Journal Title: J Biol Chem Volume: 287 Issue: 4 Pages: 2500-2508
  • DOI: 10.1074/jbc.m111.303123
  • Peer Reviewed
• [Journal Article] Preparation, characterization, and biological evaluation of 6(I), 6(IV)-di-O-[α-l-fucopyranosyl-(1→6)-2-acetamido-2-deoxy-β-d-glucopyranosyl]-cyclomaltoheptaose and 6-O-[α-l-fucopyranosyl-(1→6)-2-acetamido-2-deoxy-β-d-glucopyranosyl]-cyclomaltoheptaose (2011)
  • Author(s): Nakagawa T, Nishi Y, Kondo A, Shirai Y, Honda C, Asahi M, Tanimoto T
  • Journal Title: Carbohydr Res Volume: 346(13) Pages: 1792-800
  • Peer Reviewed
• [Journal Article] Effects of a calcineurin inhibitor, FK506, and a CCR5/CXCR3 antagonist, TAK-779, in a rat small intestinal transplantation model (2011)
  • Author(s): Takama Y, Miyagawa S, Yamamoto A, Firdawes S, Ueno T, Ihara Y, Kondo A, Matsunami K, Otsuka H, Fukuzawa M
  • Journal Title: Transpl Immunol Volume: 25(1) Pages: 49-55
  • Peer Reviewed
• [Journal Article] Preparation, characterization, and biological evaluation of 6(I),6(IV)-di-O-[α-1-fucopyranosyl-(1→6)-2-acetamido-2-deoxy-β-d-glucopyranosyl]-cyclom altoheptaose and 6-O-[α-1-fucopyranosyl-(1→6)-2-acetamido-2-deoxy-β-d-glucopyranosyl]-cyclomaltoheptaose (2011)
  • Author(s): Nakagawa T, 他
  • Journal Title: Carbohydr Res Volume: 346 Issue: 13 Pages: 1792-1800
  • DOI: 10.1016/j.carres.2011.06.013
  • Peer Reviewed
• [Journal Article] Effects of a calcineurin inhibitor, FK506, and a CCR5/CXCR3 antagonist, TAK-779, in a rat small intestinal transplantation model (2011)
  • Author(s): Takama Y, 他
  • Journal Title: Transpl Immunol Volume: 25 Issue: 1 Pages: 49-55
  • DOI: 10.1016/j.trim.2011.04.003
  • Peer Reviewed
• [Journal Article] Comparison of Methods for Profiling O-glycosylation : HUPO Human Disease Glycomics/Proteome Initiative Multi-Institutional Study of IgA1 (2010)
  • Author(s): Wada Y, Dell A, Haslam SM, Tissot B, Canis K, Azadi P, Backstrom M, Costello CE, Hansson GC, Hiki Y, Ishihara M, Ito H, Kakehi K, Karlsson N, Kato K, Kawasaki N, Khoo KH, Kobayashi K, Kolarich D, Kondo A, Lebrilla C, Nakano M, Narimatsu H, Novak J, Novotn
  • Journal Title: Mol. Cell. Proteomics Volume: 9(4) Pages: 719-27
  • Peer Reviewed
• [Journal Article] A newly cloned pig dolichyl-phosphate mannosyl-transferase for preventing the transmission of porcine endogenous retrovirus to human cells (2010)
  • Author(s): Yamamoto A, Nakatsu S, Kondo A, Asato T, Okabe M, Fukuzawa M, Miyagawa S
  • Journal Title: Transpl. Int. Volume: 23(4) Pages: 424-431
  • Peer Reviewed
• [Journal Article] Comparison of Methods for Profiling O-glycosylation : HUPO Human Disease Glycomics/Proteome Initiative Multi-Institutional Study of IgA1. (2010)
  • Author(s): Wada Y., et al.
  • Journal Title: Mol.Cell.Proteomics Volume: 9 Pages: 719-727
  • Peer Reviewed
• [Journal Article] A newly cloned pig dolichyl-phosphate mannosyl-transferase for preventing the transmission of porcine endogenous retrovirus to human cells. (2010)
  • Author(s): Yamamoto A, et al.
  • Journal Title: Transpl.Int. Volume: 23 Pages: 424-431
  • Peer Reviewed
• [Journal Article] Requirement of Fut8 for the expression of vascular endothelial growth factor receptor-2 : a new mechanism for the emphysema-like changes observed in Fut8-deficient mice (2009)
  • Author(s): Wang X, Fukuda T, Li W, Gao CX, Kondo A, Matsumoto A, Miyoshi E, Taniguchi N, Gu J
  • Journal Title: J. Biochem. Volume: 145(5) Pages: 643-51
  • NAID: 10025589041
  • Peer Reviewed
• [Journal Article] CXCL12 promotes CCR7-dependent naive T-cell trafficking to lymph nodes and Peyer's patches (2009)
  • Author(s): Zhongbin Bai, Haruko Hayasaka, Masayoshi Kobayashi, Wenzhe Li, Zijin, Guo, Myoung Ho Jang, Akihiro Kondo, Byung-il Choi, Yoichiro Iwakura, Masayuki Miyasaka
  • Journal Title: J. Immunol. Volume: 182(3) Pages: 1287-95
  • Peer Reviewed
• [Journal Article] Capillary Electrophoresis-Electro spray Ionization Mass Spectrometry for Rapid and Sensitive N-glycan Analysis of Glycoproteins as 9-Fluorenylmethyl Derivatives (2009)
  • Author(s): Miyako Nakano, Daisuke Higo, Etsuo Arai, Takatoshi Nakagawa, Kazuaki Kakehi, Naoyuki Taniguchi, and Akihiro Kondo
  • Journal Title: Glycobiology Volume: 19(2) Pages: 135-43
  • Peer Reviewed
• [Journal Article] Requirement of Fut8 for the expression of vascular endothelial growth factor receptor-2 : a new mechanism for the emphysema-like changes observed in Fut8-deficient mice (2009)
  • Author(s): Wang X, Fukuda T, Li W, Gao CX, Kondo A, Matsumoto A, Miyoshi E, Taniguchi N, Gu J.
  • Journal Title: The Journal of Biochemistry 145 Pages: 643-651
  • NAID: 10025589041
  • Peer Reviewed
• [Journal Article] Capillary Electrophoresis-Electrospray Ionization Mass Spectra metry for Rapid and Sensitive N-glycan Analysis of Glycoproteins as 9-Fluorenylmethyl Derivatives (2009)
  • Author(s): Miyako Nakano, Daisuke Higo, Etsuo Arai, Takatoshi Nakagawa, Kazuaki Kakehi, Naoyuki Taniguchi, Akihiro Kondo
  • Journal Title: Glycobiology 19 Pages: 135-143
  • Peer Reviewed
• [Presentation] 糖質分析におけるMSの可能性について (2009)
  • Author(s): 近藤昭宏
  • Organizer: 第29回日本糖質学会年会
  • Place of Presentation: 高山
  • Year and Date: 2009-09-09
• [Presentation] Analysis of AFP-L3 in Hepatocellular Carcinoma Patients (2009)
  • Author(s): Akihiro Kondo
  • Organizer: Next Generation Summit 2009
  • Place of Presentation: Washington, DC, USA
  • Year and Date: 2009-08-10
• [Presentation] Analysis of AFP-L3 in Hepatocellular Carcinoma Patients, Next Generation Summit 2009 (2009)
  • Author(s): Akihiro Kondo
  • Organizer: Next Generation Summit 2009 ; Trends in Cancer Diagnostics-Impacting Patient Care
  • Place of Presentation: The Ritz-Carlton, Washington, DC, USA
• [Presentation] The EGFR-trypsin-PAR-2 Pathway in Cell Growth and theα4β1 Integrin/VCAM-1 Interactions in Pre-B Cell (2009)
  • Author(s): Akihiro Kondo
  • Organizer: Repopulation Were Controlled by Fucosyltransferase, PepCon
  • Place of Presentation: Seoul, South Korea
• [Book] Capillary Electrophoresis of Carbohydrates (2010)
  • Author(s): Miyako Nakano, Kazuaki Kakehi, Naoyuki Taniguchi, Akihiro Kondo
  • Publisher: Humana Press
• [Book] Capillary Electrophoresis of Carbohydrates : From Monosaccharides to Complex Polysaccharides (2010)
  • Author(s): Miyako Nakano, et al.
  • Total Pages: 277
  • Publisher: Humana Press
• [Patent(Industrial Property Rights)] Composition for suppressing the expression of fucosyltransferase (2011)
  • Inventor(s): Akihiro Kondo, Wenzhe Li, Takatoshi Nakagawa, Nobuto Koyama, Naoyuki Yaniguchi, Ikunoshin Kato
  • Industrial Property Rights Holder: Osaka University, Takara Bio Inc.
  • Acquisition Date: 2011-08-23
  • Overseas