Functional analysis of carcinoma-associated fibroblasts and response to hormonal therapy in breast cancer
| Project/Area Number |
21591687
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | Research Institute for Clinical Oncology, Saitama Cancer Center |
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Principal Investigator |
YAMAGUCHI Yuri 埼玉県立がんセンター, 臨床腫瘍研究所, 主任研究員 (80166628)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 癌 / 細胞・組織 / シグナル伝達 |
|---|
| Research Abstract |
In postmenopausal breast cancers, CAFs(carcinoma-associated fibroblasts) express aromatase, and locally produce estrogen to activate the estrogen receptor(ER). We established a reporter cell system to detect the ER-activating ability of CAFs under coculture with MCF-7-E10 cells stably transfected with the ERE-GFP gene, and ER activity in breast cancer cells in primary culture by adenovirus-mediated introduction of the ERE-GFP reporter gene. In the cases in which ER-activating abilities of CAF were low, ER-activating abilities correlated with aromatase expression. In these cases, neoadjuvant hormonal therapy with an aromatase inhibitor, exemestane, decreased ER activities in tumor cells and led to a favorable clinical response. Functional analysis of CAF for individual breast cancers might be important to predict the endocrine therapy.
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Report
(4 results)
Research Products
(61 results)
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[Journal Article] Identification of a GPER/GPR30 antagonist with improved estrogen receptor counterselectivity2011
Author(s)
Dennis MK, Field AS, Burai R, Ramesh C, Petrie WK, Bologa CG, Opera TI, Yamaguchi Y, Hayashi S. SklarLA, HathawayHJ, Arterburn JB, Prossnitz ER.
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Journal Title
J Steroid Biochem Mol Biol
Volume: 127
Pages: 358-366
Related Report
Peer Reviewed
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[Journal Article] Identification of a GPER/GPR30 antagonist with improved estrogen receptor counterselectivity2011
Author(s)
Dennis MK, Field AS, Burai R, Ramesh C, Petrie WK, Bologa CG, Opera TI, Yamaguchi Y, Hayashi S, Sklar LA, Hathaway HJ, Arterburn JB, Prossnitz ER
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Journal Title
J Steroid Biochem Mol Biol.
Volume: 127
Issue: 3-5
Pages: 358-366
DOI
Related Report
Peer Reviewed
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