Determination of pathogenesis and therapeutic agents in gastric cancer patient with disseminated carcinomatosis of bone marrow

• Project/Area Number: 21591703
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: Kumamoto University
• Principal Investigator: HAYASHI Naoko 熊本大学, 医学部附属病院, 非常勤診療医師 (20452899)
• Co-Investigator(Kenkyū-buntansha): KAMOHARA Hidenobu 熊本大学, 大学院・生命科学研究部, 准教授 (90398222)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 癌幹細胞 / 胃癌骨髄癌腫症 / 遊離癌細胞(ITC)

Research Abstract

We isolated circulating tumor cells(CTCs) using CellSearch^<TM> in five cases of gastric cancer patients with disseminated carcinomatosis of bone marrow. Then, we could identify several candidate genes involved in pathogenesis of this disease, based on the result of gene expression profiling of collected CTCs. As CTCs in two patients remarkably increased and most of CTCs highly expressed HER2, we used' trastuzumab', the monoclonal antibody against HER2, combined with the chemotherapy, against these two patients. They had remission of DIC and long-term survival. Moreover, we could establish mouse xenograft model using CTCs obtained from two gastric cancer patients with disseminated carcinomatosis of bone marrow.

Research Products

• [Journal Article] Usefulness of Transcription-Reverse Transcription Concerted Reaction Method for Detecting Circulating Tumor Cells in Patients With Colorectal Cancer (2012)
  • Author(s): Sato N, Hayashi N, Imamura Y, Tanaka Y, Kinoshita K, Kurashige J, Saito S, Karashima R, Hirashima K, Nagai Y, Miyamoto Y, Iwatsuki M, Baba Y, Watanabe M, Baba H
  • Journal Title: Ann Surg Oncol Volume: (in press)
  • Peer Reviewed
• [Journal Article] The central vein access port and catheter in outpatient chemotherapy for colorectal cancer : a retrospective study of 101 patients (2012)
  • Author(s): Sawayama H, Hayashi N, Watanabe M, Takamori H, Beppu T, Baba H
  • Journal Title: Surg Today Volume: (in press)
  • NAID: 10030230224
  • Peer Reviewed
• [Journal Article] Clinical significance of circulating tumor cells, including cancer stem-like cells, in peripheral blood for recurrence and prognosis in patients with Dukes' stage B and C colorectal cancer (2011)
  • Author(s): Iinuma H, Watanabe T, Mimori K, Adachi M, Hayashi N, Tamura J, Matsuda K, Fukushima R, Okinaga K, Sasako M, Mori M
  • Journal Title: J Clin Oncol Volume: 29 Pages: 1547-1555
  • Peer Reviewed
• [Journal Article] Clinical significance of Wnt-induced secreted protein-1(WISP-1/CCN4) in esophageal squamous cell carcinoma (2011)
  • Author(s): Nagai Y, Watanabe M, Ishikawa S, Karashima R, Kurashige J, Iwagami S, Iwatsuki M, Baba Y, Imamura Y, Hayashi N, Baba H
  • Journal Title: Anticancer Res Volume: 31 Pages: 991-997
  • Peer Reviewed
• [Journal Article] Kyushu Study Group of Clinical Cancer(KSCC): A multicenter phase II clinical study of oxaliplatin, folinic acid, and 5-fluorouracil combination chemotherapy as first-line treatment for advanced colorectal cancer : a Japanese experience (2011)
  • Author(s): Baba H, Hayashi N, Emi Y, Kakeji Y, Egashira A, Oki E, Shirabe K, Toyama T, Ohga T, Yamamoto M, Hasegawa H, Kohakura F, Higashi H, Niwa K, Fujita F, Ogata Y, Kohnoe S, Inomata M, Samura H, Tokunaga S, Maehara Y
  • Journal Title: Surg Today Volume: 41 Pages: 1610-1616
  • Peer Reviewed
• [Journal Article] Laparoscopy assisted resection of an undiagnosed liver tumor and ascending colon cancer via mini median laparotomy : report of a case (2011)
  • Author(s): Mima K, Beppu T, Chikamoto A, Ishiko T, Horino K, Hayashi N, Watanabe M, Takamori H, Okabe K, Yamanaka T, Baba H
  • Journal Title: Surg Today Volume: 41 Pages: 1633-1638
  • NAID: 10030229836
  • Peer Reviewed
• [Journal Article] Reply to B. Faltas et al (2011)
  • Author(s): Iinuma H, Watanabe T, Mimori K, Adachi M, Hayashi N, Tamura J, Nozawa K, Ishihara S, Matsuda K, Fukushima R, Okinaga K, Sasako M, Mori M
  • Journal Title: J Clin Oncol Volume: 29 Pages: 2947-2948
  • Peer Reviewed
• [Journal Article] Reply to F. Crea et al (2011)
  • Author(s): Iinuma H, Watanabe T, Mimori K, Tamura J, Adachi M, Hayashi N, Nozawa K, Ishihara S, Matsuda K, Baba H, Fukushima R, Okinaga K, Sasako M, Mori M
  • Journal Title: J Clin Oncol Volume: 29 Pages: 3488-3490
  • Peer Reviewed
• [Journal Article] Reply to B.Faltas et al (2011)
  • Author(s): Iinuma H, Watanabe T, Mimori K, Adachi M, Hayashi N, Tamura J, Nozawa K, Ishihara S, Matsuda K, Fukushima R, Okinaga K, Sasako M, Mori M
  • Journal Title: J Clin Oncol Volume: 29 Issue: 21 Pages: 2947-2948
  • DOI: 10.1200/jco.2011.36.6872
  • Peer Reviewed
• [Journal Article] Reply to F.Crea et al (2011)
  • Author(s): Iinuma, H, Watanabe T, Mimori K, Tamura J, Adachi M, Hayashi N, Nozawa K, Ishihara S, Matsuda K, Baba H, Fukushima R, Okinaga K, Sasako M, Mori M
  • Journal Title: J Clin Oncol Volume: 29 Issue: 25 Pages: 3488-3490
  • DOI: 10.1200/jco.2011.37.2276
  • Peer Reviewed
• [Journal Article] A multicenter phase II clinical study of oxaliplatin, folinic acid, and 5-fluorouracil combination chemotherapy as first-line treatment for advanced colorectal cancer : a Japanese experience (2011)
  • Author(s): Baba H., et al
  • Journal Title: Surgery Today Volume: 41 Issue: 12 Pages: 1610-6
  • DOI: 10.1007/s00595-011-4589-9
  • Peer Reviewed
• [Journal Article] Clinical significance of Wnt-induced secreted protein-1 (WISP-1/CCN4) in esophageal squamous cell carcinoma (2011)
  • Author(s): Nagai Y, Watanabe M, Ishikawa S, Karashima R, Kurashige J, Iwagami S, Iwatsuki M, Baba Y, Imamura Y, Hayashi N, Baba H
  • Journal Title: Anticancer Res Volume: 31 Pages: 991-997
  • Peer Reviewed
• [Journal Article] Laparoscopy-assisted resection of an undiagnosed liver tumor and ascending colon cancer via mini median laparotomy : report of a case (2011)
  • Author(s): Mima K, Beppu T, Chikamoto A, Ishiko T, Horino K, Hayashi N, Watanabe M, Takamori H, Okabe K, Yamanaka T, Baba H
  • Journal Title: Surg Today Volume: 41 Pages: 1633-1638
  • NAID: 10030229836
  • Peer Reviewed
• [Journal Article] Extensive lymphatic spread of cancer cells in patients with thoracic esophageal squamous cell carcinoma : detection of CEA-mRNA in the three-field lymph nodes. (2010)
  • Author(s): Imamura Y, Hayashi N, Sato N, Kinoshita K, Kurashige J, Saito S, Hirashima K, Karashima R, Hiyoshi Y, Nagai Y, Watanabe M, Baba H
  • Journal Title: J Surg Oncol Volume: 102 Pages: 509-515
  • Peer Reviewed
• [Journal Article] FOLFOX enables high resectability and excellent prognosis for initially unresectable colorectal liver metastases. (2010)
  • Author(s): Beppu T, Hayashi N, Masuda T, Komori H, Horino K, Hayashi H, Okabe H, Baba Y, Kinoshita K, Akira C, Watanebe M, Takamori H, Baba H
  • Journal Title: Anticancer Res Volume: 30 Pages: 1015-1020
  • Peer Reviewed
• [Journal Article] Treatment results of FOLFOX chemotherapy before surgery for lymph node metastasis of advanced colorectal cancer with synchronous liver metastasis : the status of LN metastasis and vessel invasions at the primary site in patients who responded to FOLFOX. (2010)
  • Author(s): Sawayama H, Hayashi N, Honda S, Baba Y, Toyama E, Watanabe M, Takamori H, Beppu T, Baba H
  • Journal Title: Int J Clin Oncol Volume: 15 Pages: 70-76
  • NAID: 10027201729
  • Peer Reviewed
• [Journal Article] Adenoviral oncolytic suicide gene therapy for a peritoneal dissemination model of gastric cancer in mice (2010)
  • Author(s): Imamura Y
  • Journal Title: Ann Surg Oncol 17 Pages: 643-652
  • Peer Reviewed
• [Journal Article] MicroRNA-21 regulates proliferation and invasion in esophageal squamous cell carcinoma (2009)
  • Author(s): Hiyoshi Y
  • Journal Title: Clin Cancer Res 15 6 Pages: 1915-1922
  • Peer Reviewed
• [Journal Article] p12cdk-2AP1 is associated with tumor progression and poor prognosis in esophageal squamous cell carsinoma (2009)
  • Author(s): Imamura Y
  • Journal Title: Oncol Rep 22 Pages: 35-39
  • Peer Reviewed
• [Presentation] 消化器癌における微量癌細胞の検出とHER2陽性微量癌細胞を標的とした個別化治療への応用 (2012)
  • Author(s): 岩槻政晃、渡邊雅之、石本崇胤、岩上志朗、馬場祥史、林尚子、豊島功正、林哲、大田義一、馬場秀夫
  • Organizer: 第112回日本外科学会総会
  • Place of Presentation: 千葉幕張メッセ
• [Presentation] 切除不能進行再発大腸癌に対する治療戦略-conversion therapyを適切に施行するための指標とは? (2011)
  • Author(s): 林尚子
  • Organizer: 第111回日本外科学会総会
  • Place of Presentation: (紙上発表)(震災のため)
  • Year and Date: 2011-05-27
• [Presentation] 大腸癌イレウスに対する治療法の工夫-経肛門的イレウス管減圧後一期的腹腔鏡下手術の有用性- (2011)
  • Author(s): 林尚子、宮本裕士、齋藤誠哉、田中洋平、蔵重淳二、木下浩一、辛島龍一、平島浩太郎、長井洋平、岩上志朗、岩槻政晃、馬場祥史、渡邊雅之、馬場秀夫
  • Organizer: 第66回日本消化器外科学会総会
  • Place of Presentation: 名古屋名古屋国際会議場
• [Presentation] 切除不能進行再発大腸癌に対する治療戦略-conversion therapyを適切に施行するための指標とは?- (2011)
  • Author(s): 林尚子、佐藤伸隆、増田捻郎、齋藤誠哉、岩上志朗、宮本裕士、岩槻政晃、馬場祥史、近本亮、渡邊雅之、石河隆敏、別府透、馬場秀夫
  • Organizer: 第111回日本外科学会総会
  • Place of Presentation: (紙上発表)
• [Presentation] オンコロジストとしての外科医に求められる基礎研究とは何か (2010)
  • Author(s): 林尚子、今村裕、佐藤伸隆、斎藤誠哉、蔵重淳二、木下浩一、辛島龍一、平島浩太郎、日吉幸晴、池田貯、渡邊雅之、馬場秀夫
  • Organizer: 第110回日本外科学会定期学術集会
  • Place of Presentation: 名古屋
• [Presentation] バイオマーカーによる癌の個別化治療の試み (2010)
  • Author(s): 林尚子、平島浩太郎、齋藤誠哉、佐藤伸隆、鶴田豊、岩上志朗、宮本裕士、池田貯、渡邊雅之、馬場秀夫
  • Organizer: 第65回日本消化器外科学会総会
  • Place of Presentation: 下関
• [Presentation] 消化器外科におけるトランスレーショナル研究:臨床応用を目指した挑戦 遊離癌細胞(ITC)の同定と機能解析 (2010)
  • Author(s): 林尚子、今村裕、佐藤伸隆、斎藤誠哉、蔵重淳二、木下浩一、辛島龍一、平島浩太郎、長井洋平、岩上史朗、宮本裕士、岩槻政晃、渡邊雅之、馬場秀夫
  • Organizer: 第18回JDDW 第8回日本消化器外科学会大会
  • Place of Presentation: 横浜
• [Presentation] 胸部食道扁平上皮癌におけるリンパ節微小転移の検索とリンパ節郭清についての検討 (2009)
  • Author(s): 今村裕
  • Organizer: 第64回日本消化器外科学会総会
  • Place of Presentation: 大阪 大阪国際会議場
  • Year and Date: 2009-07-18
• [Presentation] バイオマーカーによる大腸癌に対する個別化治療の試み (2009)
  • Author(s): 林尚子
  • Organizer: 第64回日本消化器外科学会総会
  • Place of Presentation: 大阪 大阪国際会議場
  • Year and Date: 2009-07-17