Predisposition of cancer development by global analysis of DNA methylation alterations

• Project/Area Number: 21591710
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: Jichi Medical University
• Principal Investigator: SUZUKI Koichi 自治医科大学, 医学部, 助教 (70332369)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 遺伝子修飾異常 / 脱メチル化異常 / メチル化異常 / 潰瘍性大腸炎 / ヘリコバクターピロリ胃炎 / 大腸癌 / 胃癌 / 癌易罹患性 / 脱メチル化 / メチル化

Research Abstract

We evaluated the levels of demethylation alterations in normal tissues from patients who had undergone gastrectomy or colectomy due to cancer development. Our data showed that high levels of demethylation alterations were frequently seen in patients who developed synchronous or metachronous double cancers as compare with those did not. Further, we developed a novel microarray platform based on methylation-sensitive amplified fragment length polymorphism DNA fingerprinting to perform the genome-wide severance of methylation alterations, which would enable us to make biological profiles according to the several diseases including cancer.

Research Products

• [Journal Article] Array-based identification of common DNA methylation alterations in ulcerative colitis (2012)
  • Author(s): Koizumi K, Alonso S, Miyaki Y, Okada S, Ogura H, Shiiya N, Konishi F, Taya T, Perucho M, Suzuki K
  • Journal Title: Int J Oncol Volume: 40 Pages: 983-994
• [Journal Article] Aberrant methylation of the Pleckstrin and Sec7 domain-containing gene is implicated in ulcerative colitis-associated carcinogenesis through its inhibitory effect on apoptosis (2012)
  • Author(s): Okada S, Suzuki K, Takaharu K, Noda H, Kamiyama H, Maeda T, Saito M, Koizumi K, Miyaki Y, Konishi F
  • Journal Title: Int J Oncol Volume: 40 Pages: 686-694
• [Journal Article] Aberrant methylation of PSD disturbs Rac1-mediated immune responses governing neutrophil chemotaxis and apoptosis in ulcerative colitis-associated carcinogenesis (2012)
  • Author(s): Kato T, Suzuki K, Okada S, Kamiyama H, Maeda T, Saito M, Koizumi K, Miyaki Y, Konishi F
  • Journal Title: Int J Oncol Volume: 40 Pages: 942-950
• [Journal Article] The accumulation of DNA demethylation in Sat a in normal gastric tissues with Helicobacter pylori infection renders the susceptibility to gastric cancer in some fractions of individuals (2012)
  • Author(s): Masaaki Saito, Koichi Suzuki, Takaharu Kato, Shinichiro Okada, Hidenori Kamiyama, Takafumi Maeda, Kei Koizumi, Yuichiro Miyaki, Kiyozaki Hirokazu and Fumio Konishi
  • Journal Title: Oncology Reports Volume: 27 Pages: 1717-1725
• [Journal Article] DNA demethylation in normal colon tissue predicts predisposition to multiple cancers (2012)
  • Author(s): Hidenori Kamiyama, Koichi Suzuki, Takafumi Maeda, Kei Koizumi, Yuichiro Miyaki, Shinichiro Okada, Yutaka Kawamura, Johanna Samuelsson, Sergio Alonso, Fumio Konishi and Manuel Perucho
  • Journal Title: Oncogene Volume: 31 Issue: 48 Pages: 1-9
  • DOI: 10.1038/onc.2011.652
  • Peer Reviewed
• [Journal Article] Sessile serrated adenoma shares similar genetic and epigenetic features with microsatellite unstable colon cancer in a location-dependent manner (2012)
  • Author(s): Maeda T, Suzuki K, Togashi K, Nokubi M, Saito M, Tsujinaka S, Kamiyama H, Konishi F
  • Journal Title: Experimental and Therapeutic Medicine Volume: 2 Pages: 695-700
• [Journal Article] Identification of a potent epigenetic biomarker for resistance to camptothecin and poor outcome to irinotecan-based chemotherapy in colon cancer (2012)
  • Author(s): Miyaki Y, Suzuki K, Koizumi K, Kato T, Saito M, Kamiyama H, Maeda T, Shibata K, Shiya N, Konishi F
  • Journal Title: Int J Oncol Volume: 40 Pages: 217-226
• [Journal Article] 1, Array-based identification of common DNA methylation alterations in ulcerative colitis (2012)
  • Author(s): Koizumi K, Alonso S, Miyaki Y, Okada S, Ogura H, Shiiya N, Konishi F, Taya T, Perucho M, Suzuki K
  • Journal Title: Oncology Reports Volume: 40 Pages: 983-994
  • DOI: 10.3892/or.2012.1718
  • Peer Reviewed
• [Journal Article] 2, Aberrant methylation of the Pleckstrin and Sec7 domain-containing gene is implicated in ulcerative colitis-associated carcinogenesis through its inhibitory effect on apoptosis (2012)
  • Author(s): Okada S, Suzuki K, Takaharu K, Noda H, Kamiyama H, Maeda T, Saito M, Koizumi K, Miyaki Y, Konishi F
  • Journal Title: Int J Oncol Volume: 40 Pages: 686-694
  • DOI: 10.3892/ijo.2011.1231
  • Peer Reviewed
• [Journal Article] 3, Aberrant methylation of PSD disturbs Racl-mediated immune responses governing neutrophil chemotaxis and apoptosis in ulcerative colitis-associated carcinogenesis (2012)
  • Author(s): Kato T, Suzuki K, Okada S, Kamiyama H, Maeda T, Saito M, Koizumi K, Miyaki Y, Konishi F
  • Journal Title: Int J Oncol Volume: 40 Pages: 942-950
  • DOI: 10.3892/ijo.2011.1301
  • Peer Reviewed
• [Journal Article] 7, Identification of a potent epigenetic biomarker for resistance to caraptothecin and poor outcome to irinotecan-based chemotherapy in colon cancer (2012)
  • Author(s): Miyaki Y, Suzuki K, Koizumi K, Kato T, Saito M, Kamiyama H, Maeda T, Shibata K, Shiya N, Konishi F
  • Journal Title: Int J Oncol Volume: 40 Pages: 217-226
  • DOI: 10.3892/ijo.2011.1189
  • Peer Reviewed
• [Journal Article] 6, Sessile serrated adenoma shares similar genetic and epigenetic feat ures with microsatellite unstable colon cancer in a location-dependent manner (2011)
  • Author(s): Maeda T, Suzuki K, Togashi K, Nokubi M, Saito M, Tsujinaka S, Kamiyama H, Konishi F
  • Journal Title: Experimental and Therapeutic Medicine Volume: 2 Issue: 4 Pages: 695-700
  • DOI: 10.3892/etm.2011.249
  • Peer Reviewed
• [Presentation] Rac1-mediated immune systems, governing machinery of apoptosis and neutrophils chemotaxis, in ulcerative colitis-associated carcinogenesis (2011)
  • Author(s): 加藤高晴, 鈴木浩一, 岡田晋一郎, 神山英範, 前田孝文, 齊藤正昭, 宮木祐一郎, 小泉圭, 小西文雄
  • Organizer: 第7回免疫アジュバント研究会
  • Place of Presentation: 福島
  • Year and Date: 2011-10-07
• [Presentation] Sessile serrated adenomaの臨床病理学的および分子生物学的特徴と前癌病変としての意義 (2011)
  • Author(s): 前田孝文、前田孝文、前田孝文、鈴木浩一鈴木浩一鈴木浩一、冨樫一智野首光、冨樫一智野首光、冨樫一智野首光、冨樫一智野首光、冨樫一智野首光、冨樫一智野首光弘、小西文雄弘、小西文雄弘
  • Organizer: 第74回大腸癌研究会
  • Place of Presentation: 福岡
  • Year and Date: 2011-01-21
• [Presentation] Sessile serrated adenoma of the colon as a precursor of colon Cancer (2011)
  • Author(s): Konishi F, Maeda T, Suzuki K, Togashi K
  • Organizer: Colorectal Forum 2011
  • Place of Presentation: Taiwan
• [Presentation] 胃癌発癌過程におけるセントロメア領域の脱メチル化異常と染色体不安定性 (2011)
  • Author(s): 齊藤正昭、鈴木浩一、加藤高晴、前田孝文、清崎浩一、小西文雄
  • Organizer: 第22回日本消化器癌発生学会総会
  • Place of Presentation: 佐賀
• [Presentation] H. pylori感染胃粘膜組織における遺伝子修飾異常の網羅的検索によるゲノムワイド脱メチル化異常の検索 (2010)
  • Author(s): 斎藤正昭、清崎浩一、鈴木浩一、千葉文博、高田理、吉田卓義、小西文雄
  • Organizer: 第82回日本胃癌学会総会
  • Place of Presentation: 新潟
• [Presentation] 非癌部大腸粘膜のDNA脱メチル化異常を標的とした大腸癌発生の危険性の予測 (2010)
  • Author(s): 神山英範、鈴木浩一、前田孝文、小泉圭、宮木祐一郎、佐々木純一、河村裕、小西文雄
  • Organizer: 第110回日本外科学会定期学術集会
  • Place of Presentation: 名古屋
• [Presentation] Dexamethazon induced proteinの異常メチル化を指標とした抗癌剤感受性の予測と新たな治療戦略 (2010)
  • Author(s): 鈴木浩一、宮木祐一郎、神山英範、小泉圭、前田孝文、斎藤正昭、辻仲眞康、佐々木純一、溝上賢、河村裕、小西文雄
  • Organizer: 第110回日本外科学会定期学術集会
  • Place of Presentation: 名古屋
• [Presentation] Dexamethason induced proteinの異常メチル化を分子マーカーとした抗癌剤感耐性の予測と新たな抗癌剤治療戦略 (2010)
  • Author(s): 鈴木浩一、宮木祐一郎、神山英範、小泉圭、前田孝文、斎藤正昭、辻仲眞康、佐々木純一、溝上賢、河村裕、小西文雄
  • Organizer: 第73回大腸癌研究会
  • Place of Presentation: 奄美
• [Presentation] 大腸異時性多発癌発生に関わる非癌部大腸粘膜のDNA脱メチル化異常の意 (2010)
  • Author(s): 神山英範、鈴木浩一、前田孝文、辻仲眞康、佐々木純一、河村裕、小西文雄
  • Organizer: 第73回大腸癌研究会
  • Place of Presentation: 奄美
• [Presentation] 潰瘍性大腸炎の発癌にはPSDのDNA異常メチル化が関与する (2010)
  • Author(s): 岡田晋一郎、鈴木浩一、神山英範、前田孝文、小西文雄
  • Organizer: 第73回大腸癌研究会
  • Place of Presentation: 奄美
• [Presentation] Sessile Serrted Polypとマイクロサテライト不安定大腸癌の遺伝子異常 (2010)
  • Author(s): 前田孝文、鈴木浩一、冨樫一智、斎藤正昭、小西文雄
  • Organizer: 第73回大腸癌研究会
  • Place of Presentation: 奄美
• [Presentation] 右側結腸癌の組織発生におけるsessile serrated adenoma/polyp(SSA/SSP)の役割-遺伝子変化の病理組織所見による分析- (2010)
  • Author(s): 小西文雄、前田孝文、鈴木浩一、野田弘志
  • Organizer: 第6回免疫アジュバント研究会
  • Place of Presentation: 日光
• [Presentation] Global demethylation in non-cancerous colonic mucosa is likely involved in the development of multiple colon cancer (2010)
  • Author(s): 神山英範、鈴木浩一、前田孝文、小泉圭、宮木祐一郎、河村裕、小西文雄
  • Organizer: 69th annual meeting of the Japanese Cancer Association
  • Place of Presentation: 大阪
• [Presentation] Methylation status of dexamethason induced protein is a good predictive marker for CPT-11 based chemotherapy resistance (2010)
  • Author(s): 鈴木浩一、宮木祐一郎、岡田晋一郎、小泉圭、神山英範、斎藤正昭、前田孝文、河村裕、小西文雄
  • Organizer: 69th annual meeting of the Japanese Cancer Association
  • Place of Presentation: 大阪
• [Presentation] Dexamethason induced proteinの異常メチル化を分子マーカーとした抗癌剤感耐性の予測と新たな抗癌剤治療戦略 (2010)
  • Author(s): 加藤高晴、鈴木浩一、宮木祐一郎、神山英範、小泉圭、前田孝文、斎藤正昭、辻仲眞康、佐々木純一、溝上賢、河村裕、小西文雄
  • Organizer: 第21回消化器癌発生学会
  • Place of Presentation: 軽井沢
• [Presentation] H. Pylori感染とゲノムワイドな脱メチル化異常との関連の検討 (2010)
  • Author(s): 斎藤正昭、鈴木浩一、前田孝文、加藤高晴、小西文雄
  • Organizer: 第21回消化器癌発生学会
  • Place of Presentation: 軽井沢
• [Presentation] H.Pylori感染とゲノムワイドな脱メチル化異常との関連の検討 (2010)
  • Author(s): 斎藤正昭
  • Organizer: 第21回消化器癌発生学会
  • Place of Presentation: 軽井沢
• [Presentation] 大腸異時性多発癌発生に関わる非癌部大腸粘膜のDNA脱メチル化異常の意義 (2010)
  • Author(s): 神山英範
  • Organizer: 第73回大腸癌研究会
  • Place of Presentation: 奄美
• [Presentation] 潰瘍性大腸炎における粘膜組織の異常メチル化を評価し発癌の危険性を予測する (2009)
  • Author(s): 小泉圭
  • Organizer: 第51回日本消化器病学会大会
  • Place of Presentation: 京都、国立京都国際会館
  • Year and Date: 2009-10-15
• [Presentation] 潰瘍性大腸炎非癌部組織の異常メチル化と癌化 (2009)
  • Author(s): 小泉圭
  • Organizer: 第68回日本癌学会学術総会
  • Place of Presentation: 横浜、パシフィコ横浜
  • Year and Date: 2009-10-01
• [Presentation] 多発大腸癌非癌部大腸粘膜の遺伝子変化から大腸癌異時発生の危険性を予測する (2009)
  • Author(s): 神山英範, 鈴木浩一, 前田孝文, 小泉圭, 宮木祐一郎, 河村裕, 小西文雄
  • Organizer: 第64回日本消化器外科学会総会
  • Place of Presentation: 大阪
  • Year and Date: 2009-07-16
• [Presentation] 潰瘍性大腸炎に伴う発癌における易罹患性の検討 (2009)
  • Author(s): 小泉圭
  • Organizer: 第109回日本外科学会総会
  • Place of Presentation: 福岡、福岡国際会議場
  • Year and Date: 2009-04-02
• [Presentation] 大腸癌細胞株におけるDNA脱メチル化異常と染色体不安定性 (2009)
  • Author(s): 前田孝文, 鈴木浩一, 早田邦康, 河村裕, 辻仲眞康, 加納良彦, 神山英憲, 小西文雄
  • Organizer: 第109回日本外科学会定期学術集会
  • Place of Presentation: 福岡
• [Presentation] サテライトα脱メチル化異常と大腸癌易罹患性の検討 (2009)
  • Author(s): 神山英範, 鈴木浩一, 前田孝文, 野田弘志, 高田理, 河村裕, 小西文雄
  • Organizer: 第109回日本外科学会定期学術集会
  • Place of Presentation: 福岡
• [Presentation] 多発大腸癌非癌部大腸粘膜のDNA脱メチル化異常から大腸癌異時発生の危険性を予測する (2009)
  • Author(s): 神山英範, 鈴木浩一, 前田孝文, 小泉圭, 宮木祐一郎, 佐々木純一, 溝上賢, 野田弘志, 河村裕, 小西文雄
  • Organizer: 第20回日本消化器癌発生学会総会
  • Place of Presentation: 広島
• [Presentation] H. Pylori感染とゲノムワイドな脱メチル化異常の関連性
  • Author(s): 齊藤正昭、鈴木浩一、前田孝文、清崎浩一、小西文雄
  • Organizer: 第111回日本外科学会定期学術集会紙上開催
• [Book] 大腸癌の発生と遺伝子異常.大腸肛門病ハンドブック (2011)
  • Author(s): 辻仲眞康、鈴木浩一
  • Publisher: 医学書院
• [Remarks]
  • URL: http://rseeds.jichi.ac.jp/research_seeds/public/ResearcherList.php?sectionCd=70
• [Patent(Industrial Property Rights)] Dexamethasone-induced proteinの遺伝子領域、抗癌剤耐性判別キット、抗癌剤感受性回復方法、抗癌剤組成物、siRNA、アポトーシス回復組成物 (2009)
  • Inventor(s): 鈴木浩一
  • Industrial Property Rights Holder: 自治医科大学
  • Filing Date: 2009-08-27