A next step study on candidate genes regulating metastasis of colorectal cancer

• Project/Area Number: 21591719
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: Tohoku University
• Principal Investigator: MIURA Koh 東北大学, 病院, 講師 (40282074)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 大腸癌 / 転移

Research Abstract

We are analyzing orthotopically-transplanted tumors of 22 types of colorectal cancer cell lines in mouse models on epithelial-to-mesenchymal transition. Firstly E-cadherin and vimentin were immunohistochemically analyzed, and contrasting results were obtained with two cell lines : HCT116 and HT29. Currently we are examining the results for 22 types of cell lines. Next, we are analyzing the expressions of RNF125, TCF25, CRIP1, LOXL3, EN2, P2RX5 and other genes, which are candidate genes to regulate lymph node metastasis, on their expressions with RT-PCR using clinical materials, whether the results are reproducible compared with those acquired with cell lines. It seems that the expressions of RNF125 and CRIP1 are higher in more advanced cases ; on the other hand, the expressions of LOXL3, EN2, and P2RX5 genes seems to be lower in more advanced cases although the results still have to be confirmed.

Research Products

• [Journal Article] S-1 as a core anticancer fluoropyrimidine agent (2012)
  • Author(s): Miura K, Shirasaka T, Yamaue H, Sasaki I
  • Journal Title: Expert Opin Drug Deliv Volume: 9(3) Pages: 273-86
  • Peer Reviewed
• [Journal Article] Effects of ileal interposition on glucose metabolism in obese rats with diabetes (2012)
  • Author(s): Ikezawa F, Shibata C, Kikuchi D, Imoto H, Miura K, Naitoh T, Ogawa H, Sasaki I, Tsuchiya T
  • Journal Title: Surgery
  • Peer Reviewed
• [Journal Article] S-1 as a core anticancer fluoropyrimidine agent (2012)
  • Author(s): Miura K, 他
  • Journal Title: Expert Opin Drug Deliv Volume: Vol.9 Issue: 3 Pages: 273-286
  • DOI: 10.1517/17425247.2012.652945
  • Peer Reviewed
• [Journal Article] Effects of ileal interposition on glucose metabolism in obese rats with diabetes (2012)
  • Author(s): Ikezawa F, Miura K, 他
  • Journal Title: Surgery Volume: (In press) Issue: 6 Pages: 822-830
  • DOI: 10.1016/j.surg.2011.12.026
  • Peer Reviewed
• [Journal Article] Young-onset perianorectal leiomyomatosis : Report of a case (2011)
  • Author(s): Toshima M, Miura K, Ishida K, Mizoi T, Naitoh T, Ogawa H, Ohnuma S, Sasaki H, Watanabe K, Haneda S, Sase T, Kajiwara T, Matsuda Y, Shibata C, Sasaki I
  • Journal Title: Surg Today Volume: (In press)
  • Peer Reviewed
• [Journal Article] Infiltration of CD14-positive macrophages at the invasive front indicates a favorable prognosis in colorectal cancer patients with lymph node metastasis (2011)
  • Author(s): Kinouchi M, Miura K, Mizoi T, Ishida K, Fujibuchi W, Ando T, Yazaki N, Saito K, Shiiba K, Sasaki I
  • Journal Title: Hepatogastroenterology Volume: 58(106) Pages: 352-8
  • Peer Reviewed
• [Journal Article] Alternative pre-mRNA splicing in digestive tract malignancy (2011)
  • Author(s): Miura K, Fujibuchi W, Sasaki I
  • Journal Title: Cancer Sci Volume: 102(2) Pages: 309-16
  • Peer Reviewed
• [Journal Article] Inhibitor of apoptosis protein family as diagnostic markers and therapeutic targets of colorectal cancer (2011)
  • Author(s): Miura K, Fujibuchi W, Ishida K, Naitoh T, Ogawa H, Ando T, Yazaki N, Watanabe K, Haneda S, Shibata C, Sasaki I
  • Journal Title: Surg Today Volume: 41(2) Pages: 175-82
  • Peer Reviewed
• [Journal Article] Effect of viscosity of enteral nutrient on gut motility and hormone secretion in dogs (2011)
  • Author(s): Sato M, Shibata C, Kikuchi D, Ikezawa F, Imoto H, Kakyo M, Kinouchi M, Tanaka N, Miura K, Naitoh T, Ogawa H, Sasaki I
  • Journal Title: Hepatogastroenterology Volume: 58(105) Pages: 36-41
  • Peer Reviewed
• [Journal Article] Infiltration of CD14-positive macrophages at the invasive front indicates a favorable prognosis in colorectal cancer patients with lymph node metastasis (2011)
  • Author(s): Kinouchi M, Miura K, 他
  • Journal Title: Hepatogastroenterology Volume: Vol.58 Pages: 352-358
  • Peer Reviewed
• [Journal Article] Inhibitor of apoptosis protein family as diagnostic markers and therapeutic targets of colorectal cancer. (2011)
  • Author(s): Miura K, Fujibuchi W, 他
  • Journal Title: Surgery Today Volume: 41(2) Pages: 175-182
  • Peer Reviewed
• [Journal Article] Alternative pre-mRNA splicing in digestive tract malignancy. (2011)
  • Author(s): Miura K, Fujibucbi W, 他
  • Journal Title: Cancer Science Volume: 102(2) Pages: 309-316
  • Peer Reviewed
• [Journal Article] Identification of a new adhesin-like protein from Lactobacillus mucosae ME-340 with specific affinity to the human blood group A and B antigens (2010)
  • Author(s): Watanabe M, Kinoshita H, Nitta M, Yukishita R, Kawai Y, Kimura K, Taketomo N, Yamazaki Y, Tateno Y, Miura K, Horii A, Kitazawa H, Saito T
  • Journal Title: J Appl Microbiol Volume: 109(3) Pages: 927-35
  • Peer Reviewed
• [Journal Article] Intracolonic capsaicin stimulates colonic motility and defecation in conscious dogs (2010)
  • Author(s): Hayashi K, Shibata C, Nagao M, Sato M, Kakyo M, Kinouchi M, Saijo F, Miura K, Ogawa H, Sasaki I.
  • Journal Title: Surgery Volume: 147(6) Pages: 789-97
  • Peer Reviewed
• [Journal Article] 消化器疾患の病態生理cIAP2は大腸癌の治療標的となり得るか (2010)
  • Author(s): 唐澤秀明, 三浦康, 他
  • Journal Title: Progress in Medicine Volume: 30巻3号 Pages: 810-812
  • Peer Reviewed
• [Journal Article] siRNA-mediated knockdown against CDCA1 and KNTC2, both frequently overexpressed in colorectal and gastric cancers, suppresses cell proliferation and induces apoptosis (2009)
  • Author(s): Kaneko N, Miura K, Gu Z, Karasawa H, Ohnuma S, Sasaki H, Tsukamoto N, Yokoyama S, Yamamura A, Nagase H, Shibata C, Sasaki I, Horii A.
  • Journal Title: Biochem Biophys Res Commun Volume: 390 Pages: 1235-1240
  • Peer Reviewed
• [Journal Article] cIAP2 as a therapeutic target in colorectal cancer and other malignancies (2009)
  • Author(s): Miura K, Karasawa H, Sasaki I
  • Journal Title: Expert Opin Ther Targets Volume: 13(11) Pages: 1333-1345
  • Peer Reviewed
• [Journal Article] The effect of a total colectomy on the motor inhibition of the upper gut induced by intraileal stimuli in conscious dogs (2009)
  • Author(s): Nagao M, Shibata C, Funayama Y, Fukushima K, Miura K, Ogawa H, Ueno T, Kudoh K, Unno M, Sasaki I
  • Journal Title: Surg Today Volume: 39(9) Pages: 780-6
  • Peer Reviewed
• [Journal Article] Cronkhite-Canada Syndrome Complicated with Huge Intramucosal Gastric Cancer : Report of a Case (2009)
  • Author(s): Karasawa H, Miura K, Ishida K, Sase T, Kobayashi T, Kinouchi M, Okabe M, Ando T, Kaneko N, Yamamura A, Shibata C, Sasaki I
  • Journal Title: Gastric Cancer Volume: 12(2) Pages: 113-7
  • Peer Reviewed
• [Journal Article] Down-regulation of cIAP2 enhances 5-FU sensitivity through the apoptotic pathway in human colon cancer cells (2009)
  • Author(s): Karasawa H, Miura K, Fujibuchi W, Ishida K, Kaneko N, Kinouchi M, Okabe M, Ando T, Murata Y, Sasaki H, Takami K, Yamamura A, Shibata C, Sasaki I
  • Journal Title: Cancer Sci Volume: 100(5) Pages: 903-13
  • Peer Reviewed
• [Journal Article] Steroid sulfatase and estrogen sulfotransferase in colon carcinoma : regulators of intratumoral estrogen concentrations and potent prognostic factors (2009)
  • Author(s): Sato R, Suzuki T, Katayose Y, Miura K, Shiiba K, Tateno H, Miki Y, Akahira J, Kamogawa Y, Nagasaki S, Yamamoto K, Ii T, Egawa S, Evans DB, Unno M, Sasano H
  • Journal Title: Cancer Res Volume: 69(3) Pages: 914-22
  • Peer Reviewed
• [Journal Article] cIAP2 as a therapeutic target in colorectal cancer and other malignancies. (2009)
  • Author(s): Miura K, 他
  • Journal Title: Expert Opin Ther Targets. 13 Pages: 1235-1240
  • Peer Reviewed
• [Journal Article] iRNA-mediated knockdown against CDCA1 and KNTC2, both frequently overexpressed in colorectal and gastric cancers, suppresses cell proliferation and induces apoptosis. (2009)
  • Author(s): Kaneko N, Miura K, 他
  • Journal Title: Biochem Biophys Res Commun. 390 Pages: 1235-1240
  • Peer Reviewed
• [Journal Article] Differential diagnosis of rectal carcinoma using immunohistochemistry of carcinomas from other pelvic organs with NCBI literature survey and the Human Protein Atlas database
  • Author(s): Miura K, Ishida K, Fujibuchi W, Ito A, Niikura H, Ogawa H, Sasaki I
  • Journal Title: Surg Today
  • Peer Reviewed
• [Presentation] 胃癌・大腸癌における選択的pre-mRNAスプライシングの制御の異常の解明とその臨床応用 (2011)
  • Author(s): 三浦康, 大沼忍, 藤渕航, 石田和之, 佐々木巖
  • Organizer: 第49回日本癌治療学会総会学術集会
  • Place of Presentation: 名古屋
  • Year and Date: 2011-10-29
• [Presentation] 消化器癌に対する分子生物学の臨床応用(肝胆膵・上部消化管)胃癌・大腸癌における選択的pre-mRNAスプライシングの異常の解明とその臨床応用 (2011)
  • Author(s): 三浦康, 藤渕航, 大沼忍, 唐澤秀明, 木内誠, 内藤剛, 小川仁, 矢崎伸樹, 柴田近, 佐々木巖
  • Organizer: 第66回日本消化器外科学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 2011-07-15
• [Presentation] IAPファミリーおよび5-FU感受性の制御による大腸癌の治療戦略と個別化治療への応用 (2011)
  • Author(s): 三浦康, 唐澤秀明, 大沼忍, 佐々木宏之, 内藤剛, 小川仁, 木内誠, 安藤敏典, 矢崎伸樹, 渡辺和宏, 羽根田祥, 山村明寛, 佐瀬友彦, 柴田近, 佐々木
  • Organizer: 第111回日本外科学会学術集会
  • Place of Presentation: 東京
  • Year and Date: 2011-05-26
• [Presentation] IAPファミリーおよび5-FU感受性の制御による大腸癌の治療戦略と個別化治療 (2011)
  • Author(s): 三浦康, 他
  • Organizer: 第111回日本外科学会定期学術集会
  • Place of Presentation: 東京国際フォーラム(東京)
  • Year and Date: 2011-05-26
• [Presentation] A phase II multicenter trial of neoadjuvant chemotherapy FOLFOX6 in combination with bevacizumab for patients with resectable synchronous liver metastases after R0-resections of primary colorectal cancers : The interim analysis (2011)
  • Author(s): Miura K, Katayose Y, Oikawa M, Yamauchi J, Sakurai N, Musha H, Narushima Y, Nakagawa K, Egawa S, Unno M.
  • Organizer: 2011 Gastrointestinal Cancers Symposium
  • Place of Presentation: ンフランシスコ、米国
  • Year and Date: 2011-01-16
• [Presentation] Interim Report (2010)
  • Author(s): Miura K, Tsuchiya T, Tanno H, Shiiba K, Shimamura H, Suzuki Y, Nakamura R, Kimura Y, Naitoh H, Shibata C, Sasaki I
  • Organizer: 9th International Conference of the Asian Clinical Oncology Society
  • Place of Presentation: 岐阜
  • Year and Date: 2010-08-26
• [Presentation] 大腸癌の5-FU感受性を規定するpredictive markerとしてのcIAP2遺伝子の臨床への応用 (2010)
  • Author(s): 三浦康, 唐澤秀明, 山村明寛, 内藤剛, 小川仁, 安藤敏典, 矢崎伸樹, 佐瀬友彦, 柴田近, 佐々木巖
  • Organizer: 第65回日本消化器外科学会総会
  • Place of Presentation: 山口
  • Year and Date: 2010-07-14
• [Presentation] 消化器疾患における分子腫瘍マーカーの新展開大腸癌の5-FU感受性規定因子としてのcIAP2遺伝子およびIAPファミリー遺伝子群の研究とその臨床応用 (2010)
  • Author(s): 三浦康, 唐澤秀明, 佐々木巖
  • Organizer: 第96回日本消化器病学会
  • Place of Presentation: 新潟
  • Year and Date: 2010-04-24
• [Presentation] 当科での癌研究への取り組みと今後の展望 (2010)
  • Author(s): 三浦康, 藤渕航, 唐澤秀明, 大沼忍, 佐々木宏之, 矢崎伸樹, 田中直樹, 金子直征, 山村明寛, 佐瀬友彦, 小川仁, 安藤敏典, 羽根田祥, 柴田近, 鹿郷昌之, 木内誠, 西條文人, 鈴木秀幸, 木村俊一, 福島浩平, 佐々木巖
  • Organizer: 第109回日本外科学会学術集会
  • Place of Presentation: 名古屋
  • Year and Date: 2010-04-09
• [Presentation] 当科での癌研究への取り組みと今後の展望 (2010)
  • Author(s): 三浦康, 藤渕航, ほか
  • Organizer: 第110回日本外科学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 2010-04-08
• [Presentation] 臨床検体を用いた大腸癌転移を制御する遺伝子群の同定と新規治療法の開発 (2009)
  • Author(s): 三浦康, 佐々木宏之, 藤渕航, 石田和之, 矢崎伸樹, 唐澤秀明, 山村明寛, 小川仁, 木内誠, 安藤敏典, 田中直樹, 羽根田祥, 佐瀬友彦, 柴田近, 佐々木巖
  • Organizer: 第47回日本癌治療学会学術集会
  • Place of Presentation: 横浜
  • Year and Date: 2009-10-24
• [Presentation] 臨床検体を用いた大腸癌転移を制御する遺伝子群の同定と新規治療法の開発 (2009)
  • Author(s): 三浦康
  • Organizer: 第47回日本癌治療学会
  • Place of Presentation: 横浜
  • Year and Date: 2009-10-24
• [Presentation] 動物モデルと外科標本を用いた大腸癌リンパ節転移を制御する遺伝子群の同定と臨床への応用 (2009)
  • Author(s): 三浦康, 佐々木宏之, 小川仁, 岡部光規, 安藤敏典, 羽根田祥, 鹿郷昌之, 木内誠, 柴田近, 佐々木巖
  • Organizer: 第64回日本消化器外科学会総会
  • Place of Presentation: 大阪
  • Year and Date: 2009-07-17
• [Presentation] 胃癌切除標本を活用した胃癌に特異的なsplicing variantsの同定と生命科学情報の利用、臨床応用 (2009)
  • Author(s): 三浦康, 大沼忍, 金子直征, 唐澤秀明, 山村明寛, 佐瀬友彦, 堀井明, 藤渕航, Lee Christopher, 木内誠, 小川仁, 安藤敏典, 佐々木巖
  • Organizer: 第68回日本癌学会総会
  • Place of Presentation: 横浜
  • Year and Date: 2009-01-01
• [Book] Cancers (2010)
  • Author(s): Miura K, Kinouchi M, Ishida K, Fujibuchi W, Naitoh T, Ogawa H, Ando T, Yazaki N, Watanabe K, Haneda S, Shibata C, Sasaki I
  • Publisher: MDPI Publishing (Basel, Switzerland)
• [Book] Cancers 2010 (doi:10.3390), edited by Prof.Dr.Afshin Samali ; Review : 5-FU Metabolism in Cancer and Orally-Administrable 5-FU Drugs. (2010)
  • Author(s): Miura K, Kinouchi M, 他
  • Publisher: MDPI Publishing (Basel, Switzerland)