DNA diangnosis of lateral lymph nodes of rectum by HOPX methylation

• Project/Area Number: 21591732
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: Kitasato University
• Principal Investigator: NAKAMURA Takatoshi 北里大学, 医学部, 講師 (10286304)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2009: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: 大腸 / 直腸 / 直腸癌 / リンパ節転移 / HOPX / メチル化 / 側方リンパ節転移 / 予後 / 微量癌細胞 / 大腸癌 / HOP / stage III / 遺伝子発現 / 転移 / 浸潤

Research Abstract

DNA diagnosis of colorectal cancer is now challenging to develop minimal residual disease of cancer. HOPX methylation could be detected in 1/500 of cancer cells, and, however, this would not be enough to detect minimal residual disease. In order to increase the sensitivity, exploration of better markers, improvement of DNA quality or other technology must be developed. We have not yet challenged the nested PCR may be promising for this purpose(DNA shortage makes us failure to continue this experminets). CDO-1 was such candidates, however even this novel candidates were only to detect at most 20% in plasma of CRC patients, and hardly found in metastatic lymph nodes. Still ongoing for further challenge to detect the minimal residual disease.

Research Products

• [Journal Article] Laparoscopic resection of a gastrointestinal stromal tumor of the rectum after treatment with imatinib mesylate : report of a case (2012)
  • Author(s): Nakamura T, Mitomi H, Onozato W, Sato T, Ikeda A, Naito M, Ogura N, Kamata H, Ooki A, Watanabe M.
  • Journal Title: Surg Today Volume: 42 Issue: 11 Pages: 1096-1099
  • DOI: 10.1007/s00595-012-0134-8
  • NAID: 10031120774
• [Journal Article] Short-and long-term outcomes of laparoscopic surgery in patients with pathological stage II and III colon cancer (2011)
  • Author(s): Nakamura T, Mitomi H, Onozato W, Sato T, Ikeda A, Naito M, Ogura N, Kamata H, Ooki A, Watanabe M.
  • Journal Title: Hepatogastroenterology Volume: 58(112) Pages: 1947-50
• [Journal Article] Prognostic significance of preoperative bowel obstruction in stage III colorectal cancer (2011)
  • Author(s): Katoh H, Yamashita K, Wang G, Sato T, Nakamura T, Watanabe M.
  • Journal Title: Ann Surg Oncol Volume: 18(9) Pages: 2432-41
• [Journal Article] Oncological outcomes of laparoscopic surgery in elderly patients with colon cancer : a comparison of patients 64 years or younger with those 75 years or older (2011)
  • Author(s): Nakamura T, Mitomi H, Onozato W, Sato T, Ikeda A, Naito M, Ogura N, Kamata H, Ooki A, Watanabe M.
  • Journal Title: Hepatogastroenterology Volume: 58(109) Pages: 1200-4
• [Journal Article] Successful laparoscopic resection of a sacrococcygeal teratoma in an adult : report of a case (2011)
  • Author(s): Tsutsui A, Nakamura T, Mitomi H, Onozato W, Sato T, Ozawa H, Naito M, Ikeda A, Ihara A, Watanabe M.
  • Journal Title: Surg Today Volume: 41(4) Pages: 572-5
  • NAID: 10029046895
• [Journal Article] A Phase II trial of neoadjuvant preoperative chemoradiotherapy with S-1 plus irinotecan and radiation in patients with locally advanced rectal cancer : clinical feasibility and response rate (2011)
  • Author(s): Sato T, Ozawa H, Hatate K, Onosato W, Naito M, Nakamura T, Ihara A, Koizumi W, Hayakawa K, Okayasu I, Yamashita K, Watanabe M.
  • Journal Title: Int J Radiat Oncol Biol Phys Volume: 79(3) Pages: 677-83
• [Journal Article] Anastomotic leakage contributes to the risk for systemic recurrence in stage II colorectal cancer (2011)
  • Author(s): Katoh H, Yamashita K, Wang G, Sato T, Nakamura T, Watanabe M.
  • Journal Title: J Gastrointest Surg Volume: 15(1) Pages: 120-9
• [Journal Article] Genetic alterations of K-ras may reflect prognosis in stage III colon cancer patients below 60 years of age (2011)
  • Author(s): Onozato W, Yamashita K, Yamashita K, Kuba T, Katoh H, Nakamura T, Sato T, Ihara A, Okayasu I, Watanabe M.
  • Journal Title: J Surg Oncol Volume: 103(1) Pages: 25-33
• [Journal Article] Analysis of the risk factors for wound infection after surgical treatment of colorectal cancer : a matched case control study (2009)
  • Author(s): Nakamura T, Onozato W, Mitomi H, Sato T, Hatate K, Naioto M, Ihara A, Watanabe M.
  • Journal Title: Hepatogastroenterology Volume: 56(94-95) Pages: 1316-20
• [Journal Article] Prognostic significance of peritoneal tumour cells identified at surgery for colorectal cancer (2009)
  • Author(s): Katoh H, Yamashita K, Sato T, Ozawa H, Nakamura T, Watanabe M.
  • Journal Title: Br J Surg Volume: 96(7) Pages: 769-77
• [Journal Article] Retrospective, matched case-control study comparing the oncologic outcomes between laparoscopic surgery and open surgery in patients with right-sided colon cancer (2009)
  • Author(s): Nakamura T, Onozato W, Mitomi H, Naito M, Sato T, Ozawa H, Hatate K, Ihara A, Watanabe M.
  • Journal Title: Surg Today Volume: 39(12) Pages: 1040-5
  • NAID: 10027393156
• [Presentation] 大腸癌における癌抑制遺伝子HOPXの同定とAP-1(c-Fos/c-Jun)経路のクロスリンクと細胞死 (2012)
  • Author(s): 山下継史, ら
  • Organizer: 第112回日本外科学会
  • Place of Presentation: 幕張
  • Year and Date: 2012-04-14
• [Presentation] 腹腔鏡下大腸癌手術における自動縫合器を用いたリンパ節郭清の妥当性の検討 (2011)
  • Author(s): 内藤正規, 佐藤武郎, 池田篤, 小倉直人, 三浦啓寿, 筒井敦子, 原田宏輝, 中村隆俊, 渡邊昌彦
  • Organizer: 第49回日本癌治療学会学術集会
  • Place of Presentation: 名古屋
  • Year and Date: 2011-10-27
• [Presentation] 右側結腸切除におけるリンパ節郭清 (2011)
  • Author(s): 三浦啓寿, 中村隆俊, 筒井敦子, 小倉直人, 内藤正規, 池田篤, 佐藤武郎, 渡邊昌彦
  • Organizer: 第17回神奈川大腸肛門手術手技研究会学術集会
  • Place of Presentation: 横浜
  • Year and Date: 2011-06-01
• [Presentation] 結腸癌における転移陽性リンパ節個数と予後の検討 (2010)
  • Author(s): 内藤正規, 佐藤武郎, 小澤平太, 中村隆俊, 池田篤, 小野里航, 井原厚, 渡邊昌彦
  • Organizer: 第72回大腸癌研究会
  • Place of Presentation: 久留米
  • Year and Date: 2010-01-15
• [Presentation] 大腸sm, mp癌のリンパ節転移の危険因子および再発、予後の検討 (2009)
  • Author(s): 中村隆俊, 小野里航, 井原厚, 佐藤武郎, 小澤平太, 旗手和彦, 内藤正規, 渡邊昌彦
  • Organizer: 第64回日本大腸肛門病学会学術集会
  • Place of Presentation: 福岡
  • Year and Date: 2009-11-06
• [Presentation] 結腸癌の至適郭清範囲 (2009)
  • Author(s): 小野里航, 中村隆俊, 内藤正規, 旗手和彦, 小澤平太, 佐藤武郎, 井原厚, 渡邊昌彦
  • Organizer: 第64回日本消化器外科学会総会
  • Place of Presentation: 大阪
  • Year and Date: 2009-07-16