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Real time measurement of Hydroxylradical following ischemia-reperfusion injury model using rabbit liver.

Research Project

Project/Area Number 21591760
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Digestive surgery
Research InstitutionOsaka Medical College (2011)
Wakayama Medical University (2009-2010)

Principal Investigator

UCHIYAMA Kazuhisa  大阪医科大学, 医学部, 教授 (80232867)

Co-Investigator(Kenkyū-buntansha) YAMAUE Hiroki  和歌山県立医科大学, 医学部, 教授 (20191190)
UENO Masaki  和歌山県立医科大学, 医学部, 助教 (90405465)
小澤 悟  和歌山県立医科大学, 医学部, 助教 (40433354)
Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
KeywordsMicrodialysis / フリーラジカル / 虚血再灌流 / 肝切除術 / NO
Research Abstract

The aim of this study was to investigate the generation of the hydroxyl radical(・OH) in hepatic tissue in vivo after ischemia/reperfusion(I/R) injury by using a microdialysis technique. Hepatic ischemia was induced in the male rabbit liver by clamping the hepatic artery and portal vein for 20 minutes. Microdialysis probes were placed into the liver perfused with salicylate prior to, during, and after the period of clamping. In anesthetized male rabbits(n=45), ischemia was produced by the selective vascular(hepatic artery and portal vein without caudal lobe branch) occlusion for 20 min, the・OH-specific products of salycylate hydroxylation(2, 3-and 2, 5-dihydroxybenzoic acid : DHBA) in the perfusion fluid from microdalysis into the liver were measured by HPLC equipped with electrochemical detector 9 hours afterwards. There was a significant increase(p<0.05) in the intrahepatic・OH concentrations, compared to the non-ischemic control group, immediately after and seven hours after reperfusion.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (31 results)

All 2012 2011 2010 2009 Other

All Journal Article (12 results) (of which Peer Reviewed: 10 results) Presentation (16 results) Book (2 results) Remarks (1 results)

  • [Journal Article] Combined Intraoperative Use of Contrast-Enhanced Ultrasonography Imaging using a Sonazoid and Fluorescence Navigation System with Indocyanine Green during an Anatomical Hepatectomy2011

    • Author(s)
      Uchiyama K, Ueno M, Ozawa S, Kiriyama S, Shigekawa Y, Hirono, S, Kawai M, Tani M, Yamaue H.
    • Journal Title

      Langenbecks Arch Surg

      Volume: 396(7) Pages: 1101-1107

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Adjuvant Chemolipiodolization Reduces Early Recurrence Derived from Intrahepatic Metastasis of Hepatocellular Carcinoma After Hepatectomy2011

    • Author(s)
      Ueno M, Uchiyama K, Ozawa S, Hayami S, Shigekawa Y, Tani M, Yamaue H.
    • Journal Title

      Ann Surg Oncol

      Volume: 18(13) Pages: 3624-3631

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Risk factors for postoperative infectious complications after hepatectomy2011

    • Author(s)
      Uchiyama K, Ueno M, Ozawa S, Kiriyama S, Kawai M, Hirono S, Tani M, Yamaue H.
    • Journal Title

      J Hepatobiliary Pancreat Sci

      Volume: 18(1) Pages: 67-73

    • NAID

      10028173084

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Risk factors for postoperative infectious complications after hepatectomy2011

    • Author(s)
      Uchiyama K
    • Journal Title

      J Hepatobiliary Pancreat Sci

      Volume: 18 Pages: 67-73

    • NAID

      10028173084

    • Related Report
      2011 Annual Research Report 2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Impact of nodal involvement on surgical outcomes of intrahepatic cholangiocarcinoma2011

    • Author(s)
      Uchiyama K
    • Journal Title

      J Hepatobiliary Pancreat Sci

      Volume: 18 Pages: 443-452

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Combined intraoperative use of contrast-enhanced ultrasonography imaging using a sonazoid and fluorescence navigation system with indocyanine green during an anatomical hepatectomy2011

    • Author(s)
      Uchiyama K
    • Journal Title

      Langenbecks Arch Surg

      Volume: 396 Pages: 1101-1107

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 肝切除の術前における肝予備能判定式の確立とその評価2010

    • Author(s)
      内山和久, 他
    • Journal Title

      外科治療

      Volume: 102 Pages: 85-91

    • Related Report
      2010 Annual Research Report
  • [Journal Article] 胆管結石に対する胆管十二指腸(端側)吻合術の適応とその長期成績2010

    • Author(s)
      内山和久, 他
    • Journal Title

      胆と膵

      Volume: 31 Pages: 309-313

    • Related Report
      2010 Annual Research Report
  • [Journal Article] Combined use of contrast-enhanced intraoperative ultrasonography and a fluorescence navigation system for identifying hepatic metastases2010

    • Author(s)
      Uchiyama K, et al.
    • Journal Title

      World J Surg

      Volume: 34 Pages: 2953-2959

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 肝切除の術前における肝予備能判定式の確立とその評価2010

    • Author(s)
      内山和久, 他
    • Journal Title

      外科治療 102

      Pages: 85-91

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Xanthogranulomatous cholecystitis : the use of preoperative CT findings to differentiate it from gallbladder carcinoma2009

    • Author(s)
      内山和久, et al.
    • Journal Title

      J Hepatobiliary Pancreat Surg 16

      Pages: 333-338

    • NAID

      10025139179

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 当院における Stenotrophomonas maltophilia の検出状況の特徴について2009

    • Author(s)
      内山和久, 他
    • Journal Title

      日本外科感染症学会雑誌 6

      Pages: 295-600

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] 肝癌に対する肝切除術の工夫2011

    • Author(s)
      内山和久
    • Organizer
      第190回近畿外科学会
    • Place of Presentation
      大阪
    • Year and Date
      2011-11-26
    • Related Report
      2011 Final Research Report
  • [Presentation] 肝胆膵外科学会プロジェクト研究「肝内胆管癌のリンパ節転移症例の検討」2011

    • Author(s)
      内山和久
    • Organizer
      第23回日本肝胆膵外科学会
    • Place of Presentation
      東京
    • Year and Date
      2011-06-09
    • Related Report
      2011 Final Research Report
  • [Presentation] 肝内胆管癌のリンパ節転移症例の検討2011

    • Author(s)
      内山和久
    • Organizer
      第24回日本肝胆膵外科学会
    • Place of Presentation
      京王プラザホテル(東京)
    • Year and Date
      2011-06-09
    • Related Report
      2011 Annual Research Report
  • [Presentation] ドレーンを留置しない肝切除術2010

    • Author(s)
      内山和久, 他
    • Organizer
      第72回日本臨床外科学会総会
    • Place of Presentation
      横浜(主題関連演題)
    • Year and Date
      2010-11-22
    • Related Report
      2010 Annual Research Report
  • [Presentation] インフルエンザに対する今後の対策2010

    • Author(s)
      内山和久
    • Organizer
      和歌山市医師会主催市民公開講座
    • Place of Presentation
      和歌山(教育講演)
    • Year and Date
      2010-11-20
    • Related Report
      2010 Annual Research Report
  • [Presentation] Stenotrophomonas maltophiliaの検出状況の特徴について2010

    • Author(s)
      内山和久, 他
    • Organizer
      第23回日本外科感染症学会総会
    • Place of Presentation
      東京(要望演題)
    • Year and Date
      2010-11-19
    • Related Report
      2010 Annual Research Report
  • [Presentation] チームで取り組む感染症対策2010

    • Author(s)
      内山和久
    • Organizer
      第15回和歌山県病院協会学術大会
    • Place of Presentation
      和歌山(ランチョンセミナー)
    • Year and Date
      2010-11-14
    • Related Report
      2010 Annual Research Report
  • [Presentation] 今後のインフルエンザ対策2010

    • Author(s)
      内山和久
    • Organizer
      和歌山市医師会かかりつけ医研修シリーズ学術講演会
    • Place of Presentation
      和歌山(特別講演)
    • Year and Date
      2010-11-06
    • Related Report
      2010 Annual Research Report
  • [Presentation] 肝切除術後のSSIの発生防止に対するドレーン留置の意義について2010

    • Author(s)
      内山和久, 他
    • Organizer
      第8回日本消化器外科学会大会
    • Place of Presentation
      横浜(パネルディスカッション)
    • Year and Date
      2010-10-15
    • Related Report
      2010 Annual Research Report
  • [Presentation] 肝内結石症に対する外科治療2010

    • Author(s)
      内山和久, 他
    • Organizer
      難治性の肝・胆道疾患に対する調査研究
    • Place of Presentation
      東京(宿題報告)
    • Year and Date
      2010-06-05
    • Related Report
      2010 Annual Research Report
  • [Presentation] 地方都市におけるインフルエンザ対策2010

    • Author(s)
      内山和久, 他
    • Organizer
      和歌山医師会
    • Place of Presentation
      和歌山(座談会)
    • Year and Date
      2010-06-01
    • Related Report
      2010 Annual Research Report
  • [Presentation] 拡大肝切除における術前肝予備能評価式2010

    • Author(s)
      内山和久, 他
    • Organizer
      第22回日本肝胆膵外科学会
    • Place of Presentation
      仙台(ミニシンポジウム)
    • Year and Date
      2010-05-27
    • Related Report
      2010 Annual Research Report
  • [Presentation] 血管支配域に基づいた系統的肝切除を正確に施行するために2010

    • Author(s)
      内山和久, 他
    • Organizer
      第110回日本外科学会総会
    • Place of Presentation
      名古屋(ビデオワークショップ)
    • Year and Date
      2010-04-08
    • Related Report
      2010 Annual Research Report
  • [Presentation] (要望演題)肝切除術後のSSIの発生頻度について2009

    • Author(s)
      内山和久
    • Organizer
      第64回日本消化器外科学会総会
    • Place of Presentation
      大阪
    • Year and Date
      2009-07-18
    • Related Report
      2009 Annual Research Report
  • [Presentation] (ワークショップ)胆管結石の内視鏡治療後合併症および結石の再発要因の検討2009

    • Author(s)
      内山和久
    • Organizer
      第21回日本肝胆膵外科学会
    • Place of Presentation
      名古屋
    • Year and Date
      2009-06-12
    • Related Report
      2009 Annual Research Report
  • [Presentation] (ビデオ)肝門部グリソン処理による肝S8亜区域切除2009

    • Author(s)
      内山和久
    • Organizer
      第21回日本肝胆膵外科学会
    • Place of Presentation
      名古屋
    • Year and Date
      2009-06-10
    • Related Report
      2009 Annual Research Report
  • [Book] 消化器外科NURSING 2012春季増刊号2012

    • Author(s)
      林道廣, 内山和久
    • Publisher
      Medica出版
    • Related Report
      2011 Final Research Report
  • [Book] 肝内結石症の診療ガイド2011

    • Author(s)
      内山和久
    • Publisher
      文光堂
    • Related Report
      2011 Final Research Report
  • [Remarks] ホームページ(大阪医科大学一般・消化器外科)

    • URL

      http://www.Osaka-med.ac.jp/deps/sur/

    • Related Report
      2011 Final Research Report

URL: 

Published: 2009-04-01   Modified: 2016-04-21  

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