| Project/Area Number |
21591771
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
DOI Ryuichiro 京都大学, 医学研究科, 非常勤講師 (20301236)
KODAMA Sota 京都大学, 医学研究科, 非常勤講師 (10583875)
KAWAGUCHI Yoshiya 京都大学, iPS細胞研究所, 教授 (60359792)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2010: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2009: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Keywords | ptf1a / 急性膵炎 / 膵発生 / Notch / 膵外分泌組織維持 / 膵再生 / ptfla / 膵炎 / 膵炎モデル / プロモータ解析 |
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| Research Abstract |
Based on a hypothesis that embryonic organogenic pathway is reactivated in the regenerative process after injury in the exocrine pancreatic tissue, we aimed to reveal an involvement of ptf1a transcription factor in the tissue regeneration after Cerulein induced acute pancreatitis model in mice. First, we tested the dosage of ptf1a by injecting Cerulein to the hypomorphic ptf1a mice(ptf1a-cbll/cbll). However, we could not determine the role of ptf1a in the regenerative process since most of the mice died after Cerulein treatment. Then we inactivated ptf1a in adult pancreatic duct cells in Sox9 CreER ; ptf1a floxed ; ROSA26r mice. Surprisingly, we observed lack of labeled acinar cell even in the non-Cerulein treated mice indicating that ptf1a is indispensable in the physiological organ maintenance of the exocrine pancreas tissue.
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