Identification of MicroRNA related to EMT of pancreas cancer, and elucidation of the miRNA control facility by Redox

• Project/Area Number: 21591776
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: Kyushu University
• Principal Investigator: TAKAHATA Shunichi 九州大学, 大学病院, 助教 (50437779)
• Co-Investigator(Kenkyū-buntansha): UEDA Junji 九州大学, 大学病院, 助教 (90529801) ; TSUTSUMI Kosuke 九州大学, 医学研究院, 共同研究員 (00467937) ; OHUCHIDA Kenoki 九州大学, 大学院・医学研究院, 講師 (20452708) ; 永井 英司 九州大学, 医学研究院, 准教授 (30264021)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 膵臓外科学 / 膵癌 / EMT / miRNA / Redox

Research Abstract

We analyzed over 100 surgical tissues of pancreatic cancer by using frozen sections. Several MicroRNA(miRNA) were identified as prognostic markers. Furthermore, we detected that precursor of miR-17-5p and that of miR-10b enhanced proliferation and invasion of pancreatic cancer cells. Meanwhile, we detected that precursor of miR-200c suppressed invasion of pancreatic cancer cells.

Research Products

• [Journal Article] MicroRNA expression as a predictive marker for gemcitabine response after surgical resection of pancreatic cancer (2011)
  • Author(s): Ohuchida K, Mizumoto K, Kayashima T, Fujita H, Moriyama T, Ohtsuka T, Ueda J, Nagai E, Hashizume M, Tanaka M
  • Journal Title: Ann Surg Oncol Volume: 18(8) Pages: 2381-2387
• [Journal Article] MicroRNA-10b is overexpressed in pancreatic cancer, promotes its invasiveness, and correlates with a poor prognosis (2011)
  • Author(s): Nakata K, Ohuchida K, Mizumoto K, Kayashima T, Ikenaga N, Sakai H, Lin C, Fujita H, Otsuka T, Aishima S, Nagai E, Oda Y, Tanaka M
  • Journal Title: Surgery Volume: 150 Pages: 916-922
• [Journal Article] 膵がん・膵内分泌腫瘍の最近の動向 (2011)
  • Author(s): 大内田研宙, 藤田逸人, 水元一博, 田中雅夫
  • Journal Title: 膵がんの分子生物学的解析の現状腫瘍内科 Volume: 7(2) Pages: 131-136
• [Journal Article] 分子生物学的研究に基づく膵癌の診断と治療-臨床応用を目指して (2011)
  • Author(s): 藤田逸人, 大内田研宙, 水元一博, 田中雅夫
  • Journal Title: 福岡医学雑誌 Volume: 102(6) Pages: 203-214
  • NAID: 40018954520
• [Journal Article] 【膵がん・膵内分泌腫瘍の最近の動向】膵がんの分子生物学的解析の現状 (2011)
  • Author(s): 大内田研宙, ほか
  • Journal Title: 腫瘍内科 Volume: 7(2) Pages: 131-136
• [Journal Article] 分子生物学的研究に基づく膵癌の診断と治療-臨床応用を目指して- (2011)
  • Author(s): 藤田逸人, ほか
  • Journal Title: 福岡医学雑誌 Volume: 102(6) Pages: 203-214
• [Journal Article] MicroRNA-203 expression as a new prognostic marker of pancreatic adenocarcinoma (2010)
  • Author(s): Ikenaga N, Ohuchida K, Mizumoto K, Yu J, Kayashima T, Sakai H, Fujita H, Nakata K, Tanaka M
  • Journal Title: Annals of Surgical Oncology Volume: 17 Pages: 3120-3128
• [Journal Article] MicroRNA miR-17-5p is overexpressed in pancreatic cancer (2010)
  • Author(s): Yu J, Ohuchida K, Mizumoto K, Fujita H, Nakata K, Tanaka M
  • Journal Title: associated with a poor prognosis and involved in cancer cell proliferation and invasion Cancer Biology & Therapy Volume: 10 Pages: 748-757
• [Journal Article] MicroRNA, has-miR-200c, is an independent prognostic factor in pancreatic cancer and its upregulation inhibits pancreatic cancer invasion but increases cell proliferation (2010)
  • Author(s): Yu J, Ohuchida K, Mizumoto K, Sato N, Kayashima T, Fujita H, Nakata K, Tanaka M
  • Journal Title: Molecular Cancer Volume: 9
• [Journal Article] MicroRNA miR-17-5p is overexpressed in pancreatic cancer, associated with a poor prognosis and involved in cancer cell proliferation and invasion (2010)
  • Author(s): Jun Yu, et al.
  • Journal Title: Cancer Biology & Therapy Volume: 10 Pages: 748-757
  • Peer Reviewed
• [Journal Article] MicroRNA, has-miR-200c, is an independent prognostic factor in pancreatic cancer and its upregulation inhibits pancreatic cancer invasion but increases cell proliferation (2010)
  • Author(s): Jun Yu, et al.
  • Journal Title: Molecular Cancer Volume: 9
  • Peer Reviewed
• [Journal Article] Predictors of the presence of concomitant invasive ductal carcinoma in intraductal papillary neoplasm of the pancreas (2010)
  • Author(s): Ingkakul T, Sadakari Y, Ienaga J, Satoh N, Takahata S, Tanaka M.
  • Journal Title: Ann Surg 251 Pages: 70-75
  • Peer Reviewed
• [Journal Article] Prevention of iatrogenic bile duct injuries in difficult laparoscopie cholecystectomies : is the naso-biliary drain the answer? (2009)
  • Author(s): Chandika A, Sadakari Y, Kitada H, Ienaga J, Tanabe R, Takahata S, Nabae T Tanaka M
  • Journal Title: J Hepatobiliary Pancreat Surg 16 Pages: 458-462
  • Peer Reviewed
• [Journal Article] Midkine mRNA is overexpressed in pancreatic cancer (2009)
  • Author(s): Ohhashi S, Ohuchida K, Mizumoto K, Egami T, Yu J, Cui L, Toma H, Takahata S, Nabae T, Tanaka M
  • Journal Title: Dig Dis Sci. 54 Pages: 811-815
  • Peer Reviewed
• [Presentation] MicroRNA-203 expression as a new prognostic marker of pancreatic cancer(膵癌予後因子としてのmicroRNA-203) (2010)
  • Author(s): 池永直樹,大内田研宙,水元一博,坂井寛,田中雅夫
  • Organizer: 69th Annual Meeting of the Japanese Cancer Association
• [Presentation] MicroRNA-203 expression as a new prognostic marker of pancreatic cancer Joint Meeting of the International (2010)
  • Author(s): Ikenaga N, Sakai H, Ohuchida K, Mizumoto K, Tanaka M
  • Organizer: Association of Pancreatology and the Japan Pancreas Society
  • Place of Presentation: Fukuoka, Japan
• [Presentation] MicroRNA-21(miR-21) Modulates Biologic Functions of Pancreatic Cancer Cells Including Their Proliferation, Invation and Chemoresistance (2010)
  • Author(s): Moriyama T, Ohuchida K, Mizumoto K, Tanaka M
  • Organizer: Joint Meeting of the International Association of Pancreatology and the Japan Pancreas Society
  • Place of Presentation: Fukuoka, Japan
• [Presentation] Tanaka M, MicroRNA-10b is a Useful Prognostic Marker in Pancreatic Cancer and Promotes Its Invasiveness Joint Meeting of the International (2010)
  • Author(s): Nakata K, Ohuchida K, Mizumoto K, Kozono S, Zhao M, Kayashima T, Ikenaga N, Cui L, Sakai H, Onimaru M, Moriyama T, Fujita H, Ohtsuka T, Nagai E, Tanaka M
  • Organizer: Association of Pancreatology and the Japan Pancreas Society
  • Place of Presentation: Fukuoka, Japan
• [Presentation] Micro RNA10bは膵臓癌において浸潤,悪性度に関与している (2010)
  • Author(s): 仲田興平,大内田研宙,水元一博,小薗真吾,萱島理,池永直樹,余俊,藤田逸人,大塚隆生,永井英司,田中雅夫
  • Organizer: 第110回日本外科学会定期学術集会
• [Presentation] MicroRNA-203 expression as a new prognostic marker of pancreatic cancer (2010)
  • Author(s): Ikenaga N, et al.
  • Organizer: Joint Meeting of the International Association of Pancreatology and the Japan Pancreas Society
  • Place of Presentation: Fukuoka, Japan
• [Presentation] Matrix Metalloproteinase 7(MMP7)is over expressed in invasive intraductal papillary mutinous neoplasm(IPMNs)of the pancreas (2009)
  • Author(s): Tsutsumi K, Ohtsuka T, Sadakari Y, Nakata K, Ohuchida K, Takahata S, Mizumoto K, Tanaka M
  • Organizer: 40^<th> anniversary APA/JPS joint annual meeting
  • Place of Presentation: Hawaii
  • Year and Date: 2009-11-05
• [Presentation] 膵癌におけるマイクロRNA-200cの発現とその発現増強による浸潤能抑制効果 (2009)
  • Author(s): 余俊,大内田研宙,森山大樹,崔林,趙茗,佐藤典宏,水元一博,田中雅夫
  • Organizer: 第109回日本外科学会定期学術集会
• [Presentation] 網羅的発現解析による膵癌の治療抵抗性に関わるmicro RNAの同定 (2009)
  • Author(s): 大内田研宙,水元一博,余俊,森山大樹,佐藤典宏,当間宏樹,永井英司,橋爪誠,田中雅夫
  • Organizer: 第109回日本外科学会定期学術集会