Profiling and functional analyses of microRNAs in human intrahepatic cholangiocarcinoma

• Project/Area Number: 21591784
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: Nippon Medical School
• Principal Investigator: ISHIKAWA Tomoko 日本医科大学, 医学部, 助教 (70212850)
• Co-Investigator(Kenkyū-buntansha): YOSHIDA Hiroshi 日本医科大学, 医学部, 准教授 (60246999) ; KIKUCHI Kunio 日本医科大学, 医学部, 講師 (70374676)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 外科学 / 胆道 / 癌 / microRNA / 肝胆道 / 胆管癌

Research Abstract

We examined functional analysis of biliary epithelial cell-specific miRNAs in a human intrahepatic cholangiocarcinoma cell line(HuCCT1). We identified a growth factor receptor binding protein(GRB), which is implicated in tyrosine kinase signal transduction, as a biliary epithelial cell-specific miRNA target. It is likely that upregulation of the GRB protein by downregulation of the biliary epithelial cell-specific miRNAs contributes to motility in HuCCT1.

Research Products

• [Journal Article] Real-time PCR-based analysis of the human bile microRNAome identifies miR-9 as a potential diagnostic biomarker for biliary tract cancer (2011)
  • Author(s): Shigehara K, Yokomuro S, Ishibashi O, Mizuguchi Y, Arima Y, Kawahigashi Y, Kanda T, Akagi I, Tajiri T, Yoshida H, Takizawa T, Uchida E.
  • Journal Title: PLoS One Volume: 6 Issue: 8 Pages: e23584-e23584
  • DOI: 10.1371/journal.pone.0023584
  • Peer Reviewed
• [Journal Article] Sequencing and bioinformatics-based analyses of the microRNA transcriptome in hepatitis B-related hepatocellular carcinoma. (2011)
  • Author(s): Mizuguchi Y, Mishima T, Yokomuro S, Arima Y, Kawahigashi Y, Shigehara K, Kanda T, Yoshida H, Uchida E, Tajiri T, Takizawa T.
  • Journal Title: PLoS One Volume: 6 Issue: 1 Pages: e15304-e15304
  • DOI: 10.1371/journal.pone.0015304
  • Peer Reviewed
• [Journal Article] 絨毛と胎盤をめぐる新知見【絨毛研究最前線4】絨毛細胞におけるマイクロRNA (2011)
  • Author(s): 菊池邦生, 石橋宰, 竹下俊行, 瀧澤俊広
  • Journal Title: 臨床婦人科産科 Volume: 65 Pages: 254-259
• [Journal Article] Identification of obstructive jaundice-related microRNAs in mouse liver (2010)
  • Author(s): Kanda T, Ishibashi O, Kawahigashi Y, Mishima T, Kosuge T, Mizuguchi Y, Shimizu T, Arima Y, Yokomuro Y, Yoshida H, Tajiri T, Uchida E, Takizawa T.
  • Journal Title: Hepato-Gastroenterology Volume: 102-103 Pages: 1013-1023
  • Peer Reviewed
• [Journal Article] マイクロRNA 3.女性生殖器(胎盤)におけるマイクロRNA (2010)
  • Author(s): 石橋宰, 羅善順, 石川朋子, 小管拓治, 倉品隆平, 石川源, 朝倉啓文, 泉章夫, 大口昭英, 松原茂樹, 竹下俊行, 瀧澤俊広
  • Journal Title: 産婦人科の実際 Volume: 59 Pages: 2077-2083
• [Journal Article] Identification of obstructive jaundice-related microRNAs in mouse liver (2010)
  • Author(s): Tomohiro Kanda
  • Journal Title: Hepato-Gastroenterology Volume: 57 Pages: 1013-1023
  • Peer Reviewed
• [Journal Article] Identification of obstructive jaundice-related microRNAs in mouse liver (2010)
  • Author(s): Tomohiro Kanda
  • Journal Title: Hepato-Gastroenterology 57(in press)
  • Peer Reviewed
• [Presentation] 胎盤特異的microRNAの発現に関与するCpG配列のメチル化解析 (2012)
  • Author(s): 菊池邦生, 曲光瑾, 羅善順, 瀧澤俊広
  • Organizer: 第117回日本解剖学会総会・全国学術集会
  • Place of Presentation: 山梨
  • Year and Date: 2012-03-26
• [Presentation] 肝内胆管癌で発現が抑制されているmiRNAの機能解析の試み (2011)
  • Author(s): 菊池邦生, 川東豊, 石川朋子, 三嶋拓也, 水口義昭, 石橋宰, 吉田寛, 内田英二, 瀧澤俊広
  • Organizer: 第116回日本解剖学会総会・全国学術集会
  • Place of Presentation: 神奈川
  • Year and Date: 2011-03-30
• [Presentation] 肝内胆管癌で発現が抑制されているmiRNAの機能解析の試み (2011)
  • Author(s): 菊池邦生
  • Organizer: 第116回日本解剖学会
  • Place of Presentation: 東京
  • Year and Date: 2011-03-30
• [Presentation] 肝内胆管癌で発現が抑制されているmiRNAの同定と機能の解析 (2010)
  • Author(s): 菊池邦生, 川東豊, 石川朋子, 三嶋拓也, 水口義昭, 石橋宰, 吉田寛, 内田英二, 瀧澤俊広
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
  • Place of Presentation: 兵庫
  • Year and Date: 2010-12-09
• [Presentation] 肝内胆管癌で発現が抑制されているmiRNAの同定と機能の解析 (2010)
  • Author(s): 菊池邦生
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-09
• [Presentation] MicroRNA profiling of human intrahepatic cholangiocarcinoma cell lines reveals biliary epithelial cell specific microRNAs (2010)
  • Author(s): 川東豊
  • Organizer: 第78回日本医科大学医学会 平成21年度優秀論文賞記念講演
  • Place of Presentation: 東京
  • Year and Date: 2010-09-04
• [Presentation] 胆管上皮細胞で発現するmiRNAのプロファイリングと機能解析の試み (2010)
  • Author(s): 菊池邦生, 川東豊, 石川朋子, 三嶋拓也, 水口義昭, 石橋宰, 吉田寛, 内田英二, 田尻孝, 瀧澤俊広
  • Organizer: 第12回日本RNA学会年会
  • Place of Presentation: 東京
  • Year and Date: 2010-07-28
• [Presentation] 胆管上皮細胞に発現しているmiRNAの機能解析 (2010)
  • Author(s): 菊池邦生, 川東豊, 石川朋子, 三嶋拓也, 水口義昭, 石橋宰, 吉田寛, 内田英二, 田尻孝, 瀧澤俊広
  • Organizer: 第115回日本解剖学会総会・全国学術集会
  • Place of Presentation: 岩手
  • Year and Date: 2010-03-28
• [Presentation] 胆管上皮細胞に発現するmiRNAの機能解析 (2010)
  • Author(s): 菊池邦生
  • Organizer: 第115回日本解剖学会総会・全国学術集会
  • Place of Presentation: 盛岡
  • Year and Date: 2010-03-28
• [Remarks] 川東豊、三嶋拓也、水口義昭、有馬保生、横室茂樹、神田知洋、石橋宰、吉田寛、田尻孝、瀧澤俊広. MicroRNA profiling of human intrahepatic cholangiocarcinoma cell lines reveals biliary epithelial cell-specific microRNAs. J Nippon Med Sch. 77 : 188-197, 2009.査読有優秀論文賞日本医科大学医学会(第78回日本医科大学医学会総会) 2010年9月4日東京学術