Therapeutic angiogenesis using sustained release of platelet-rich plasma from biodegradable gelatin hydrogel

• Project/Area Number: 21591796
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Thoracic surgery
• Research Institution: Kyoto University
• Principal Investigator: MARUI Akira 京都大学, 医学研究科, 准教授 (60402856)
• Co-Investigator(Kenkyū-buntansha): MIWA Senri 京都大学, 医学研究科, 講師 (10359812)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 血管新生治療 / 多血小板血漿 / 増殖因子 / 血管新生療法

Research Abstract

We used platelet-rich-plasma(PRP) as a novel method for therapeutic angiogenesis of limb ischemia. We showed that PRP contains several potent angiogenic growth factors such as SDF-1, PDGF-BB, VEGF, and bFGF. In addition, sustained release of PRP from biodegradable gelatin hydrogel facilitated angiogenesis both in normal and diabetic murine ischemic limb. This technique may be a promising option for therapeutic angiogenesis in the clinical settings.

Research Products

• [Journal Article] Therapeutic Treatment with Sustained-Release Platelet-Rich Plasma Restores Blood Perfusion by Augmenting Ischemia-Induced Angiogenesis and Arteriogenesis in Diabetic Mice (2011)
  • Author(s): Bir SC, et al
  • Journal Title: Journal of Vascular Research Volume: 48 Issue: 3 Pages: 195-205
  • DOI: 10.1159/000318779
  • Peer Reviewed
• [Journal Article] 下肢血管新生療法の臨床応用 (2011)
  • Author(s): 丸井晃, 他
  • Journal Title: 日本医事新報 Volume: 4,553
• [Journal Article] Therapeutic Treatment with Sustained-Release Platelet-Rich Plasma Restores Blood Perfusion by Augmenting Ischemia-Induced Angiogenesis and Arteriogenesis in Diabetic Mice. (2011)
  • Author(s): Bir SC, et al.
  • Journal Title: Journal of Vascular Research Volume: 48 Pages: 195-195
  • Peer Reviewed
• [Journal Article] Remote Post conditioning may Attenuate Ischaemia-Reperfusion Injury in the Murine Hind limb Through Adenosine Receptor Activation (2010)
  • Author(s): Tsubota H, Marui A, Esaki J, Bir SC, Ikeda T, Sakata R
  • Journal Title: Eur J Vasc Endovasc Surg Volume: 40 Pages: 804-9
• [Journal Article] Therapeutic Treatment with Sustained-Release Platelet-Rich Plasma Restores Blood Perfusion by Augmenting Ischemia-Induced Angiogenesis and Arteriogenesis in Diabetic Mice (2010)
  • Author(s): Bir SC, Esaki J, Marui A, Sakaguchi H, Kevil CG, Ikeda T, Komeda M, Tabata Y, Sakata R
  • Journal Title: J Vasc Res Volume: 48 Pages: 195-205
• [Journal Article] Remote Postconditioning may Attenuate IschaemiaeReperfusion Injury in the Murine Hindlimb Through Adenosine Receptor Activation (2010)
  • Author(s): H.Tsubota, et al.
  • Journal Title: European Journal of Vascular and Endovascular Surgery Volume: 40 Pages: 804-804
  • Peer Reviewed
• [Journal Article] Angiogenic properties of sustained release platelet-rich plasma : characterization in-vitro and in the ischemic hind limb of the mouse (2009)
  • Author(s): Bir SC, Esaki J, Marui A, Yamahara K, Tsubota H, Ikeda T, Sakata R
  • Journal Title: J Vasc Surg Volume: 50 Pages: 570-879
• [Journal Article] Local sustained release of prostaglandin E1 induces neovascularization in murine hindlimbischemia (2009)
  • Author(s): Esaki J, Sakaguchi H, Marui A, Bir SC, Arai Y, Huang Y, Tsubota H, Kanaji T, Ikeda T, Sakata R
  • Journal Title: Circ J Volume: 73 Pages: 1330-6
  • NAID: 10025932902
• [Journal Article] Angiogenic properties of sustained release platelet-rich plasma : characterization in-vitro and in the ischemic hind limb of the mouse. (2009)
  • Author(s): Bir SC, et al.
  • Journal Title: Journal of Vascular Surgery 50 Pages: 870-9
  • Peer Reviewed
• [Journal Article] Local sustained release of prostaglandin E1 induces neovascularization in murine hindlimb ischemia. (2009)
  • Author(s): Esaki J, et al.
  • Journal Title: Circulation Journal 73 Pages: 1330-6
  • Peer Reviewed
• [Journal Article] New therapeutic approach for impaired arteriogenesis in diabetic mouse hind limb ischemia (2008)
  • Author(s): Bir SC, Fujita M, Marui A, Hirose K, Arai Y, Sakaguchi H, Huang Y, Esaki J, Ikeda T, Tabata Y, Komeda M
  • Journal Title: Circ J Volume: 72(4) Pages: 633-40
  • NAID: 110006656909
• [Journal Article] 患者まで届く再生医療生体吸収性担体を用いた重症下肢虚血に対する血管新生療法
  • Author(s): 丸井晃, 田畑泰彦, 柳茂樹, 山本雅哉, 米澤淳, 桂敏也, 手良向聡, 横出正之, 清水章, 中尾一和, 坂田隆造
  • Journal Title: 再生医療 Volume: 10巻1号 Pages: 37-44
  • NAID: 40018724611
• [Journal Article] 下肢血管新生療法の臨床応用
  • Author(s): 丸井晃, 坂田隆造
  • Journal Title: 日本医事新報 Volume: 4553号 Pages: 62-63
• [Journal Article] 【疾患治療とドラッグデリバリーシステム】細胞増殖因子徐放化による血管新生治療
  • Author(s): 山本雅哉, 丸井晃, 坂田隆造, 田畑泰彦
  • Journal Title: 細胞 Volume: 44巻2 Pages: 64-67
• [Presentation] Biomaterial-based Therapeutic Angiogenesis in Cardiovascular Field (2012)
  • Author(s): Marui A, et al
  • Organizer: Asian-Pacific Meeting of Vasculitis and ANCA Workshop 2012
  • Place of Presentation: Tokyo (tokyo conference Center shinagawa)
  • Year and Date: 2012-03-28
• [Presentation] bFGF徐放化ゼラチンハイドロゲルによる重症下肢虚血に対する血管新生療法 (2011)
  • Author(s): 熊谷基之、丸井晃、田畑泰彦、武田崇秀、山本雅哉、米澤淳、池田隆文、桂敏也、川上浩司、横出正之、清水章、坂田隆造
  • Organizer: 第1回DDS徐放化再生医療研究会
  • Place of Presentation: 東京
  • Year and Date: 2011-12-03
• [Presentation] 臨床を目指した高分子バイオマテリアルのDDS新規バイオマテリアル"LYDEX"を用いた心臓血管外科領域におけるDDS (2011)
  • Author(s): 丸井晃, 玄丞烋, 坂田隆造
  • Organizer: 第33回日本バイオマテリアル学会大会
  • Place of Presentation: 京都市
  • Year and Date: 2011-11-22
• [Presentation] ゼラチンハイドロゲルによるDDSを応用した心血管再生医療-From bench to bedside- (2011)
  • Author(s): 丸井晃
  • Organizer: 第4回北和脈管疾患連携の会
  • Place of Presentation: 奈良市
  • Year and Date: 2011-09-03
• [Presentation] 重症下肢虚血に対する血管新生療法の中間報告 (2011)
  • Author(s): 小田基之、丸井晃、武田崇秀、坂田隆造
  • Organizer: 第11回比叡山ワークショップ
  • Place of Presentation: 京都市
  • Year and Date: 2011-06-04
• [Presentation] Biomaterial-based Regenerative Therapy in Cardiovascular Field (2011)
  • Author(s): Marui A, Tabata Y, Sakata R.
  • Organizer: 1st Meeting of Asia-Pacific Federation of Inflammation and Regeneration
  • Place of Presentation: Kyoto
  • Year and Date: 2011-06-02
• [Presentation] Biomaterial-based Regenerative Therapy in Cardiovascular Field (2011)
  • Author(s): Marui A, et al
  • Organizer: 1st meeting of Asian-Pacific Federation of Inflammation and Regeneration
  • Place of Presentation: Kyoto (International Conference Center Kyoto)
  • Year and Date: 2011-06-02
• [Presentation] bFGF徐放化ゼラチンハイドロゲルによる血管新生療法の長期成績および医薬品市販化・保険診療化の試み (2011)
  • Author(s): 丸井晃, 田畑泰彦, 柳茂樹, 山本雅哉, 米澤淳, 桂敏也, 横出正之, 清水章, 坂田隆造
  • Organizer: 第39回日本血管外科学会学術集会
  • Place of Presentation: 那覇市
  • Year and Date: 2011-04-21
• [Presentation] bFGF徐放化ゼラチンハイドロゲルによる血管新生療法の長期成績および医薬品市販化・保険診療化の試み (2011)
  • Author(s): 丸井晃, 他
  • Organizer: 第39回日本血管外科学会学術総会
  • Place of Presentation: 那覇(沖縄コンベンションセンター・ラグナガーデンホテル)
  • Year and Date: 2011-04-21
• [Presentation] 心血管トランスレーショナルリサーチ(TR)医薬品市販化・保険診療化を目指した試み (2011)
  • Author(s): 丸井晃, 田畑泰彦, 柳茂樹, 山本雅哉, 米澤淳, 桂敏也, 清水章, 坂田隆造
  • Organizer: 第41回日本心臓血管外科学会学術集会
  • Place of Presentation: 東京都
  • Year and Date: 2011-02-23
• [Presentation] bFGF徐放化ゼラチンハイドロゲルによる下肢血管新生療法 (2010)
  • Author(s): 丸井晃、柳茂樹、坂田隆造
  • Organizer: 文部科学省橋渡し研究支援推進プログラム平成21年度成果報告会
  • Place of Presentation: 東京都
• [Presentation] Remote post conditioning may attenuate ischemia-reperfusion injury in a murine hind limb through activation of adenosine receptors (2009)
  • Author(s): Tsubota H, Marui A, Miwa S, Nakata T, Minakata K, Yamazaki K, Ikeda T, Sakata R
  • Organizer: American Heart Association, Scientific Sessions
  • Place of Presentation: Orlando, USA
• [Presentation] platelet rich plasma(PRP)の除放による血管新生治療 (2009)
  • Author(s): Tsubota H, Bir SC, Marui A, Esaki J, Tabata Y, Fujita M, Ikeda T, Sakata R
  • Organizer: 第39回日本心臓血管外科学会学術集会
  • Place of Presentation: 富山県
• [Presentation] Remote postconditioning may attenuate ischemia-reperfusion injury in a murine hind limb through activation of adenosine receptors (2009)
  • Author(s): Tsubota H, et al.
  • Organizer: American Heart Association, Scientific Session 2009
  • Place of Presentation: Orlando, Florida, USA
• [Presentation] New Therapeutic Approach for Impaired Arteriogenesis in Diabetic Mouse Hind limb Ischemia (2008)
  • Author(s): Bir SC, Fujita M, Hirose K, Marui A, Arai Y, Sakaguchi H, Esaki J, Huang Y, Ikeda T, Tabata Y, Komeda M
  • Organizer: セロトニン研究会
  • Place of Presentation: 京都
• [Presentation] Sustained Release of Platelet-Rich Plasma, Cocktail of Potent Angiogenic Cytokines, Restores Blood Perfusion by Recruitment of Bone Marrow Derived Hematopoietic stem Cells in the Mouse Hind Limb Ischemia (2008)
  • Author(s): Bir SC, Marui A, Esaki J, Yamahara K, Tsubota H, Fujita M, Tabata Y, Ikeda T
  • Organizer: American Heart Association Scientific Sessions
  • Place of Presentation: New Orleans, The US
• [Presentation] An Evolving Approach of Therapeutic Angiogenesis with Sustained Release of Platelet-Rich Plasma in Diabetic Mouse Hind Limb Ischemia (2008)
  • Author(s): Bir SC, Akira Marui, Esaki J, Tsubota H, Fujita M, Tabata Y, Ikeda T
  • Organizer: American Heart Association Scientific Sessions
  • Place of Presentation: New Orleans, The US
• [Presentation] A Novel Therapeutic Angiogenesis With Sustained Delivery Form Of Platelet-Rich Plasma In Mouse Hind limb Ischemia Model (2008)
  • Author(s): Bir SC, Akira Marui, Jiro Esaki, Hisashi Sakaguchi, Yoshio Arai, Keiichi Hirose, Tadashi Ikeda, Yasuhiko Tabata, Masatoshi Fujita, FAHA.; Masashi Komeda
  • Organizer: ACC Annual Scientific Session
  • Place of Presentation: Chicago, The US
• [Book] ものづくりからみる再生医療-細胞研究・創薬・治療- (2011)
  • Author(s): 田畑泰彦
  • Total Pages: 282
  • Publisher: シーエムシー出版
• [Remarks]
  • URL: http://www.kyoto-cvs.com/